Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldtE and lpp are separated by 62 nucleotides lpp and pykF are separated by 310 nucleotides pykF and fumD are separated by 556 nucleotides
HEPCGN_05665: ldtE - L,D-transpeptidase LdtE, at 1,006,567 to 1,007,571
ldtE
HEPCGN_05670: lpp - murein lipoprotein Lpp, at 1,007,634 to 1,007,870
lpp
HEPCGN_05675: pykF - pyruvate kinase PykF, at 1,008,181 to 1,009,593
pykF
HEPCGN_05680: fumD - fumarate hydratase FumD, at 1,010,150 to 1,010,359
fumD
Position (kb)
1008
1009
1010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1007.318 kb on + strand, within ldtE at 1007.319 kb on - strand, within ldtE at 1007.319 kb on - strand, within ldtE at 1007.331 kb on - strand, within ldtE at 1007.443 kb on + strand, within ldtE at 1007.514 kb on - strand at 1007.551 kb on - strand at 1007.570 kb on - strand at 1007.580 kb on - strand at 1007.754 kb on - strand, within lpp at 1007.826 kb on - strand, within lpp at 1007.826 kb on - strand, within lpp at 1007.875 kb on - strand at 1007.875 kb on - strand at 1007.894 kb on + strand at 1007.906 kb on - strand at 1008.077 kb on - strand at 1008.077 kb on - strand at 1008.211 kb on - strand at 1008.248 kb on - strand at 1008.248 kb on - strand at 1008.319 kb on - strand at 1008.319 kb on - strand at 1008.425 kb on + strand, within pykF at 1008.679 kb on - strand, within pykF at 1009.014 kb on + strand, within pykF at 1009.535 kb on - strand at 1009.697 kb on - strand at 1009.895 kb on - strand at 1010.021 kb on - strand at 1010.025 kb on - strand at 1010.025 kb on - strand at 1010.049 kb on + strand at 1010.112 kb on + strand at 1010.115 kb on - strand at 1010.115 kb on - strand at 1010.122 kb on + strand at 1010.143 kb on - strand at 1010.251 kb on + strand, within fumD at 1010.252 kb on - strand, within fumD at 1010.271 kb on + strand, within fumD at 1010.271 kb on + strand, within fumD at 1010.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 1,007,318 + ldtE HEPCGN_05665 0.75 +1.4 1,007,319 - ldtE HEPCGN_05665 0.75 -0.8 1,007,319 - ldtE HEPCGN_05665 0.75 -0.9 1,007,331 - ldtE HEPCGN_05665 0.76 -0.1 1,007,443 + ldtE HEPCGN_05665 0.87 +1.0 1,007,514 - -3.2 1,007,551 - -0.9 1,007,570 - +0.6 1,007,580 - -0.0 1,007,754 - lpp HEPCGN_05670 0.51 -0.8 1,007,826 - lpp HEPCGN_05670 0.81 -1.6 1,007,826 - lpp HEPCGN_05670 0.81 -1.4 1,007,875 - -1.0 1,007,875 - -3.4 1,007,894 + -0.1 1,007,906 - -0.2 1,008,077 - +0.2 1,008,077 - +0.1 1,008,211 - -2.0 1,008,248 - -0.3 1,008,248 - +0.2 1,008,319 - -1.8 1,008,319 - +0.2 1,008,425 + pykF HEPCGN_05675 0.17 +0.4 1,008,679 - pykF HEPCGN_05675 0.35 +0.4 1,009,014 + pykF HEPCGN_05675 0.59 +2.6 1,009,535 - -1.6 1,009,697 - -0.1 1,009,895 - -0.4 1,010,021 - +0.1 1,010,025 - -0.2 1,010,025 - +1.2 1,010,049 + -0.5 1,010,112 + +1.1 1,010,115 - -0.5 1,010,115 - +0.2 1,010,122 + +0.7 1,010,143 - +0.2 1,010,251 + fumD HEPCGN_05680 0.48 -0.3 1,010,252 - fumD HEPCGN_05680 0.49 +0.1 1,010,271 + fumD HEPCGN_05680 0.58 +0.8 1,010,271 + fumD HEPCGN_05680 0.58 +0.1 1,010,433 - +1.0
Or see this region's nucleotide sequence