Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeS and HEPCGN_03335 are separated by 14 nucleotides HEPCGN_03335 and HEPCGN_03340 are separated by 81 nucleotides HEPCGN_03340 and HEPCGN_03345 are separated by 393 nucleotides
HEPCGN_03330: yeeS - UPF0758 protein YeeS, at 572,779 to 573,255
yeeS
HEPCGN_03335: HEPCGN_03335 - restriction endonuclease, at 573,270 to 573,749
_03335
HEPCGN_03340: HEPCGN_03340 - DUF945 domain-containing protein, at 573,831 to 574,652
_03340
HEPCGN_03345: HEPCGN_03345 - hypothetical protein, at 575,046 to 575,528
_03345
Position (kb) 573
574
575 Strain fitness (log2 ratio) -1
0
1 at 572.872 kb on + strand, within yeeS at 573.259 kb on - strand at 573.288 kb on - strand at 573.385 kb on + strand, within HEPCGN_03335 at 574.519 kb on + strand, within HEPCGN_03340 at 574.653 kb on - strand at 574.830 kb on + strand at 574.836 kb on - strand at 574.863 kb on - strand at 575.023 kb on + strand at 575.039 kb on + strand at 575.065 kb on + strand at 575.176 kb on + strand, within HEPCGN_03345 at 575.177 kb on - strand, within HEPCGN_03345 at 575.177 kb on - strand, within HEPCGN_03345 at 575.177 kb on - strand, within HEPCGN_03345 at 575.266 kb on - strand, within HEPCGN_03345 at 575.354 kb on + strand, within HEPCGN_03345 at 575.389 kb on - strand, within HEPCGN_03345 at 575.391 kb on + strand, within HEPCGN_03345 at 575.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 572,872 + yeeS HEPCGN_03330 0.19 +0.7 573,259 - +0.6 573,288 - -0.3 573,385 + HEPCGN_03335 0.24 -1.6 574,519 + HEPCGN_03340 0.84 -0.8 574,653 - +0.2 574,830 + -0.5 574,836 - -0.9 574,863 - +0.2 575,023 + +0.4 575,039 + -0.5 575,065 + -0.9 575,176 + HEPCGN_03345 0.27 +0.3 575,177 - HEPCGN_03345 0.27 -0.5 575,177 - HEPCGN_03345 0.27 +1.5 575,177 - HEPCGN_03345 0.27 -0.1 575,266 - HEPCGN_03345 0.46 -0.1 575,354 + HEPCGN_03345 0.64 +0.1 575,389 - HEPCGN_03345 0.71 -0.4 575,391 + HEPCGN_03345 0.71 +0.2 575,543 + +0.8
Or see this region's nucleotide sequence