Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaD and wcaE are separated by 10 nucleotides wcaE and wcaF are separated by 15 nucleotides wcaF and HEPCGN_03045 are separated by 26 nucleotides
HEPCGN_03030: wcaD - putative colanic acid polymerase WcaD, at 514,958 to 516,175
wcaD
HEPCGN_03035: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 516,186 to 516,932
wcaE
HEPCGN_03040: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 516,948 to 517,496
wcaF
HEPCGN_03045: HEPCGN_03045 - GDP-mannose 4,6-dehydratase, at 517,523 to 518,644
_03045
Position (kb) 516
517 Strain fitness (log2 ratio) -1
0
1
2 at 515.194 kb on + strand, within wcaD at 515.199 kb on - strand, within wcaD at 515.239 kb on + strand, within wcaD at 515.240 kb on - strand, within wcaD at 515.293 kb on + strand, within wcaD at 515.313 kb on - strand, within wcaD at 515.387 kb on - strand, within wcaD at 515.389 kb on + strand, within wcaD at 515.429 kb on + strand, within wcaD at 515.566 kb on + strand, within wcaD at 515.880 kb on - strand, within wcaD at 516.180 kb on - strand at 516.194 kb on + strand at 516.195 kb on - strand at 516.353 kb on + strand, within wcaE at 516.365 kb on - strand, within wcaE at 516.365 kb on - strand, within wcaE at 516.600 kb on - strand, within wcaE at 517.039 kb on + strand, within wcaF at 517.204 kb on + strand, within wcaF at 517.223 kb on - strand, within wcaF at 517.264 kb on + strand, within wcaF at 517.571 kb on + strand at 517.571 kb on + strand at 517.615 kb on + strand at 517.615 kb on + strand at 517.616 kb on - strand at 517.619 kb on - strand at 517.724 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.773 kb on - strand, within HEPCGN_03045 at 517.823 kb on + strand, within HEPCGN_03045 at 517.907 kb on + strand, within HEPCGN_03045
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 515,194 + wcaD HEPCGN_03030 0.19 +0.1 515,199 - wcaD HEPCGN_03030 0.20 -0.4 515,239 + wcaD HEPCGN_03030 0.23 +0.7 515,240 - wcaD HEPCGN_03030 0.23 +0.1 515,293 + wcaD HEPCGN_03030 0.28 +0.6 515,313 - wcaD HEPCGN_03030 0.29 -0.7 515,387 - wcaD HEPCGN_03030 0.35 -0.0 515,389 + wcaD HEPCGN_03030 0.35 +0.2 515,429 + wcaD HEPCGN_03030 0.39 -0.5 515,566 + wcaD HEPCGN_03030 0.50 -0.2 515,880 - wcaD HEPCGN_03030 0.76 -0.3 516,180 - +0.6 516,194 + +1.5 516,195 - +2.7 516,353 + wcaE HEPCGN_03035 0.22 -1.4 516,365 - wcaE HEPCGN_03035 0.24 +0.6 516,365 - wcaE HEPCGN_03035 0.24 +1.5 516,600 - wcaE HEPCGN_03035 0.55 -1.0 517,039 + wcaF HEPCGN_03040 0.17 +0.6 517,204 + wcaF HEPCGN_03040 0.47 +0.0 517,223 - wcaF HEPCGN_03040 0.50 -0.2 517,264 + wcaF HEPCGN_03040 0.58 +0.1 517,571 + +0.1 517,571 + -0.3 517,615 + -0.8 517,615 + -0.4 517,616 - -1.2 517,619 - -0.2 517,724 - HEPCGN_03045 0.18 -0.5 517,773 - HEPCGN_03045 0.22 +0.1 517,773 - HEPCGN_03045 0.22 -0.3 517,773 - HEPCGN_03045 0.22 +0.5 517,823 + HEPCGN_03045 0.27 +1.6 517,907 + HEPCGN_03045 0.34 +0.4
Or see this region's nucleotide sequence