Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolB and pal are separated by 34 nucleotides pal and cpoB are separated by 9 nucleotides cpoB and Lys_trna are separated by 164 nucleotides
HEPCGN_01465: tolB - Tol-Pal system protein TolB, at 222,538 to 223,830
tolB
HEPCGN_01470: pal - peptidoglycan-associated lipoprotein Pal, at 223,865 to 224,386
pal
HEPCGN_01475: cpoB - cell division protein CpoB, at 224,396 to 225,187
cpoB
HEPCGN_01480: Lys_trna - tRNA-Lys(ttt), at 225,352 to 225,427
_trna
Position (kb) 223
224
225 Strain fitness (log2 ratio) -2
-1
0
1
2 at 222.871 kb on + strand, within tolB at 222.871 kb on + strand, within tolB at 222.920 kb on + strand, within tolB at 223.096 kb on + strand, within tolB at 223.096 kb on + strand, within tolB at 223.183 kb on + strand, within tolB at 223.184 kb on - strand, within tolB at 223.402 kb on - strand, within tolB at 223.449 kb on + strand, within tolB at 223.449 kb on + strand, within tolB at 223.450 kb on - strand, within tolB at 223.482 kb on + strand, within tolB at 223.483 kb on - strand, within tolB at 223.515 kb on + strand, within tolB at 223.515 kb on + strand, within tolB at 223.516 kb on - strand, within tolB at 223.518 kb on + strand, within tolB at 223.572 kb on + strand, within tolB at 223.578 kb on - strand, within tolB at 223.640 kb on + strand, within tolB at 223.708 kb on + strand at 223.708 kb on + strand at 223.709 kb on - strand at 223.709 kb on - strand at 223.752 kb on + strand at 223.832 kb on - strand at 223.835 kb on - strand at 224.079 kb on - strand, within pal at 224.079 kb on - strand, within pal at 224.096 kb on + strand, within pal at 224.203 kb on + strand, within pal at 224.306 kb on + strand, within pal at 224.329 kb on - strand, within pal at 224.352 kb on - strand at 224.382 kb on - strand at 224.405 kb on - strand at 224.421 kb on + strand at 224.446 kb on + strand at 224.449 kb on - strand at 224.831 kb on + strand, within cpoB at 224.843 kb on - strand, within cpoB at 224.927 kb on + strand, within cpoB at 224.928 kb on - strand, within cpoB at 224.946 kb on - strand, within cpoB at 224.967 kb on - strand, within cpoB at 224.967 kb on - strand, within cpoB at 224.999 kb on + strand, within cpoB at 225.000 kb on - strand, within cpoB at 225.114 kb on - strand at 225.114 kb on - strand at 225.114 kb on - strand at 225.114 kb on - strand at 225.134 kb on + strand at 225.135 kb on - strand at 225.185 kb on + strand at 225.186 kb on - strand at 225.186 kb on - strand at 225.241 kb on + strand at 225.301 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 222,871 + tolB HEPCGN_01465 0.26 -2.7 222,871 + tolB HEPCGN_01465 0.26 -1.5 222,920 + tolB HEPCGN_01465 0.30 -2.0 223,096 + tolB HEPCGN_01465 0.43 +0.1 223,096 + tolB HEPCGN_01465 0.43 -1.4 223,183 + tolB HEPCGN_01465 0.50 -1.7 223,184 - tolB HEPCGN_01465 0.50 -1.1 223,402 - tolB HEPCGN_01465 0.67 +2.0 223,449 + tolB HEPCGN_01465 0.70 -1.3 223,449 + tolB HEPCGN_01465 0.70 -2.1 223,450 - tolB HEPCGN_01465 0.71 -2.0 223,482 + tolB HEPCGN_01465 0.73 +0.2 223,483 - tolB HEPCGN_01465 0.73 -0.8 223,515 + tolB HEPCGN_01465 0.76 -1.5 223,515 + tolB HEPCGN_01465 0.76 +1.7 223,516 - tolB HEPCGN_01465 0.76 -1.5 223,518 + tolB HEPCGN_01465 0.76 -0.7 223,572 + tolB HEPCGN_01465 0.80 -2.6 223,578 - tolB HEPCGN_01465 0.80 -2.4 223,640 + tolB HEPCGN_01465 0.85 -1.1 223,708 + -2.6 223,708 + -1.5 223,709 - -2.5 223,709 - -0.5 223,752 + -0.7 223,832 - -1.3 223,835 - -2.1 224,079 - pal HEPCGN_01470 0.41 -1.7 224,079 - pal HEPCGN_01470 0.41 -2.0 224,096 + pal HEPCGN_01470 0.44 -1.2 224,203 + pal HEPCGN_01470 0.65 +0.4 224,306 + pal HEPCGN_01470 0.84 -1.2 224,329 - pal HEPCGN_01470 0.89 +0.0 224,352 - -2.2 224,382 - +0.6 224,405 - +1.1 224,421 + -0.5 224,446 + +0.3 224,449 - -0.2 224,831 + cpoB HEPCGN_01475 0.55 -0.3 224,843 - cpoB HEPCGN_01475 0.56 -0.1 224,927 + cpoB HEPCGN_01475 0.67 -0.2 224,928 - cpoB HEPCGN_01475 0.67 -0.4 224,946 - cpoB HEPCGN_01475 0.69 +0.1 224,967 - cpoB HEPCGN_01475 0.72 -0.1 224,967 - cpoB HEPCGN_01475 0.72 -0.1 224,999 + cpoB HEPCGN_01475 0.76 +0.3 225,000 - cpoB HEPCGN_01475 0.76 -0.4 225,114 - +0.1 225,114 - +0.2 225,114 - -0.6 225,114 - -0.9 225,134 + -0.7 225,135 - -0.6 225,185 + -0.3 225,186 - +0.1 225,186 - -0.3 225,241 + +0.4 225,301 + -1.3
Or see this region's nucleotide sequence