Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaB and ubiF are separated by 145 nucleotides ubiF and Gln_trna are separated by 215 nucleotides Gln_trna and Met_trna are separated by 48 nucleotides Met_trna and Gln_trna are separated by 15 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Leu_trna are separated by 23 nucleotides Leu_trna and Met_trna are separated by 8 nucleotides
HEPCGN_01005: miaB - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 128,003 to 129,427
miaB
HEPCGN_01010: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 129,573 to 130,748
ubiF
HEPCGN_01015: Gln_trna - tRNA-Gln(ctg), at 130,964 to 131,038
_trna
HEPCGN_01020: Met_trna - tRNA-Met(cat), at 131,087 to 131,163
_trna
HEPCGN_01025: Gln_trna - tRNA-Gln(ttg), at 131,179 to 131,253
_trna
HEPCGN_01030: Gln_trna - tRNA-Gln(ttg), at 131,288 to 131,362
_trna
HEPCGN_01035: Leu_trna - tRNA-Leu(tag), at 131,386 to 131,470
_trna
HEPCGN_01040: Met_trna - tRNA-Met(cat), at 131,479 to 131,555
_trna
Position (kb) 129
130
131 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 128.584 kb on + strand, within miaB at 128.925 kb on + strand, within miaB at 128.926 kb on - strand, within miaB at 128.940 kb on + strand, within miaB at 128.941 kb on - strand, within miaB at 128.941 kb on - strand, within miaB at 128.949 kb on + strand, within miaB at 128.949 kb on + strand, within miaB at 128.949 kb on + strand, within miaB at 128.950 kb on - strand, within miaB at 128.950 kb on - strand, within miaB at 129.046 kb on + strand, within miaB at 129.047 kb on - strand, within miaB at 129.135 kb on + strand, within miaB at 129.136 kb on - strand, within miaB at 129.136 kb on - strand, within miaB at 129.222 kb on - strand, within miaB at 129.379 kb on - strand at 129.406 kb on + strand at 129.407 kb on - strand at 129.407 kb on - strand at 129.470 kb on + strand at 129.475 kb on + strand at 129.543 kb on + strand at 129.550 kb on + strand at 129.550 kb on + strand at 129.664 kb on + strand at 129.962 kb on - strand, within ubiF at 130.136 kb on + strand, within ubiF at 130.254 kb on + strand, within ubiF at 130.646 kb on + strand at 130.731 kb on + strand at 130.731 kb on + strand at 130.746 kb on + strand at 130.771 kb on - strand at 130.894 kb on - strand at 131.276 kb on - strand at 131.368 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.689 kb on + strand at 131.700 kb on + strand at 131.730 kb on + strand at 131.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 128,584 + miaB HEPCGN_01005 0.41 +0.4 128,925 + miaB HEPCGN_01005 0.65 +1.0 128,926 - miaB HEPCGN_01005 0.65 -0.0 128,940 + miaB HEPCGN_01005 0.66 +0.9 128,941 - miaB HEPCGN_01005 0.66 -0.3 128,941 - miaB HEPCGN_01005 0.66 +0.6 128,949 + miaB HEPCGN_01005 0.66 -0.1 128,949 + miaB HEPCGN_01005 0.66 -1.5 128,949 + miaB HEPCGN_01005 0.66 +0.2 128,950 - miaB HEPCGN_01005 0.66 -1.0 128,950 - miaB HEPCGN_01005 0.66 +1.2 129,046 + miaB HEPCGN_01005 0.73 -0.4 129,047 - miaB HEPCGN_01005 0.73 -0.4 129,135 + miaB HEPCGN_01005 0.79 +0.1 129,136 - miaB HEPCGN_01005 0.80 -0.4 129,136 - miaB HEPCGN_01005 0.80 +0.2 129,222 - miaB HEPCGN_01005 0.86 -1.0 129,379 - -0.2 129,406 + +0.1 129,407 - +0.2 129,407 - -0.4 129,470 + +0.4 129,475 + -1.6 129,543 + -0.6 129,550 + -2.2 129,550 + -0.4 129,664 + -1.6 129,962 - ubiF HEPCGN_01010 0.33 -2.4 130,136 + ubiF HEPCGN_01010 0.48 -1.4 130,254 + ubiF HEPCGN_01010 0.58 -2.9 130,646 + +1.2 130,731 + +0.1 130,731 + +1.1 130,746 + -0.1 130,771 - +1.0 130,894 - +0.3 131,276 - -1.2 131,368 - -0.4 131,688 - -4.3 131,688 - +0.2 131,688 - -0.2 131,689 + +0.3 131,700 + +0.6 131,730 + +0.3 131,730 + +0.5
Or see this region's nucleotide sequence