Strain Fitness in Escherichia coli ECRC102 around NIAGMN_28705

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrepA and tap overlap by 8 nucleotidestap and repA2 are separated by 235 nucleotidesrepA2 and NIAGMN_28710 are separated by 239 nucleotidesNIAGMN_28710 and NIAGMN_28715 are separated by 37 nucleotidesNIAGMN_28715 and yncB are separated by 45 nucleotides NIAGMN_28690: repA - incFII family plasmid replication initiator RepA, at 45,755 to 46,612 repA NIAGMN_28695: tap - RepA leader peptide Tap, at 46,605 to 46,679 tap NIAGMN_28705: repA2 - Replication regulatory protein repA2, at 46,915 to 47,169 repA2 NIAGMN_28710: NIAGMN_28710 - W0003, at 47,409 to 47,747 _28710 NIAGMN_28715: NIAGMN_28715 - transcriptional regulator, at 47,785 to 47,994 _28715 NIAGMN_28720: yncB - Uncharacterized endonuclease, at 48,040 to 48,501 yncB Position (kb) 46 47 48Strain fitness (log2 ratio) -2 -1 0 1 2 3at 45.976 kb on + strand, within repAat 45.976 kb on + strand, within repAat 46.185 kb on + strand, within repAat 46.185 kb on + strand, within repAat 46.186 kb on - strand, within repAat 46.186 kb on - strand, within repAat 46.186 kb on - strand, within repAat 46.206 kb on + strand, within repAat 46.218 kb on + strand, within repAat 46.578 kb on - strandat 46.590 kb on + strandat 46.591 kb on - strandat 46.591 kb on - strandat 46.591 kb on - strandat 46.627 kb on - strand, within tapat 46.680 kb on - strandat 46.682 kb on - strandat 46.685 kb on + strandat 46.716 kb on + strandat 46.723 kb on + strandat 46.723 kb on + strandat 46.799 kb on - strandat 46.832 kb on - strandat 46.875 kb on - strandat 46.905 kb on - strandat 46.945 kb on + strand, within repA2at 47.014 kb on - strand, within repA2at 47.014 kb on - strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.038 kb on + strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.062 kb on - strand, within repA2at 47.062 kb on - strand, within repA2at 47.117 kb on + strand, within repA2at 47.118 kb on - strand, within repA2at 47.118 kb on - strand, within repA2at 47.118 kb on - strand, within repA2at 47.183 kb on + strandat 47.187 kb on + strandat 47.187 kb on + strandat 47.187 kb on + strandat 47.188 kb on - strandat 47.188 kb on - strandat 47.188 kb on - strandat 47.213 kb on - strandat 47.213 kb on - strandat 47.213 kb on - strandat 47.215 kb on + strandat 47.216 kb on - strandat 47.287 kb on - strandat 47.287 kb on - strandat 47.297 kb on - strandat 47.306 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.319 kb on - strandat 47.319 kb on - strandat 47.321 kb on + strandat 47.322 kb on - strandat 47.334 kb on + strandat 47.335 kb on - strandat 47.414 kb on + strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.450 kb on + strand, within NIAGMN_28710at 47.450 kb on + strand, within NIAGMN_28710at 47.547 kb on - strand, within NIAGMN_28710at 47.572 kb on - strand, within NIAGMN_28710at 47.572 kb on - strand, within NIAGMN_28710at 47.587 kb on + strand, within NIAGMN_28710at 47.588 kb on - strand, within NIAGMN_28710at 47.604 kb on + strand, within NIAGMN_28710at 47.605 kb on - strand, within NIAGMN_28710at 47.605 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.673 kb on + strand, within NIAGMN_28710at 47.770 kb on - strandat 47.960 kb on - strand, within NIAGMN_28715at 48.045 kb on + strandat 48.152 kb on + strand, within yncBat 48.153 kb on - strand, within yncBat 48.161 kb on + strand, within yncBat 48.161 kb on + strand, within yncBat 48.162 kb on - strand, within yncBat 48.163 kb on + strand, within yncBat 48.164 kb on - strand, within yncBat 48.164 kb on - strand, within yncB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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45,976 + repA NIAGMN_28690 0.26 +1.4
45,976 + repA NIAGMN_28690 0.26 +0.6
46,185 + repA NIAGMN_28690 0.50 +1.0
46,185 + repA NIAGMN_28690 0.50 +1.7
46,186 - repA NIAGMN_28690 0.50 +2.3
46,186 - repA NIAGMN_28690 0.50 -1.3
46,186 - repA NIAGMN_28690 0.50 +1.1
46,206 + repA NIAGMN_28690 0.53 -0.1
46,218 + repA NIAGMN_28690 0.54 +1.7
46,578 - +1.9
46,590 + -0.8
46,591 - +2.0
46,591 - +1.4
46,591 - -0.4
46,627 - tap NIAGMN_28695 0.29 +0.5
46,680 - -1.4
46,682 - -1.0
46,685 + +0.6
46,716 + +0.0
46,723 + -0.3
46,723 + -0.3
46,799 - -0.0
46,832 - -0.1
46,875 - +0.2
46,905 - +0.3
46,945 + repA2 NIAGMN_28705 0.12 -0.3
47,014 - repA2 NIAGMN_28705 0.39 -2.1
47,014 - repA2 NIAGMN_28705 0.39 -1.9
47,015 + repA2 NIAGMN_28705 0.39 -1.0
47,015 + repA2 NIAGMN_28705 0.39 -1.0
47,015 + repA2 NIAGMN_28705 0.39 -1.6
47,015 + repA2 NIAGMN_28705 0.39 -2.5
47,016 - repA2 NIAGMN_28705 0.40 -1.1
47,016 - repA2 NIAGMN_28705 0.40 -0.5
47,016 - repA2 NIAGMN_28705 0.40 -0.9
47,016 - repA2 NIAGMN_28705 0.40 +0.0
47,016 - repA2 NIAGMN_28705 0.40 -1.1
47,016 - repA2 NIAGMN_28705 0.40 -0.5
47,038 + repA2 NIAGMN_28705 0.48 -0.3
47,039 - repA2 NIAGMN_28705 0.49 -0.9
47,039 - repA2 NIAGMN_28705 0.49 -1.0
47,039 - repA2 NIAGMN_28705 0.49 -1.9
47,039 - repA2 NIAGMN_28705 0.49 +0.5
47,039 - repA2 NIAGMN_28705 0.49 -0.5
47,039 - repA2 NIAGMN_28705 0.49 -1.9
47,039 - repA2 NIAGMN_28705 0.49 +0.5
47,062 - repA2 NIAGMN_28705 0.58 -0.0
47,062 - repA2 NIAGMN_28705 0.58 -1.2
47,117 + repA2 NIAGMN_28705 0.79 +0.4
47,118 - repA2 NIAGMN_28705 0.80 -0.6
47,118 - repA2 NIAGMN_28705 0.80 -2.1
47,118 - repA2 NIAGMN_28705 0.80 -0.2
47,183 + -1.0
47,187 + -0.3
47,187 + -1.3
47,187 + -1.3
47,188 - +0.7
47,188 - -0.3
47,188 - +0.5
47,213 - -0.0
47,213 - -0.5
47,213 - -0.1
47,215 + -1.0
47,216 - -1.4
47,287 - -1.4
47,287 - -0.3
47,297 - -0.7
47,306 + +0.2
47,318 + +1.4
47,318 + +1.7
47,318 + -0.1
47,318 + -0.7
47,318 + -1.2
47,318 + -2.0
47,318 + +0.9
47,319 - +0.1
47,319 - +0.3
47,321 + +0.6
47,322 - +0.2
47,334 + -1.6
47,335 - +0.4
47,414 + -0.3
47,415 - -0.4
47,415 - -0.6
47,415 - -2.1
47,415 - +0.0
47,450 + NIAGMN_28710 0.12 +1.0
47,450 + NIAGMN_28710 0.12 +0.6
47,547 - NIAGMN_28710 0.41 +0.2
47,572 - NIAGMN_28710 0.48 +0.3
47,572 - NIAGMN_28710 0.48 +0.6
47,587 + NIAGMN_28710 0.53 -0.8
47,588 - NIAGMN_28710 0.53 +0.0
47,604 + NIAGMN_28710 0.58 +1.7
47,605 - NIAGMN_28710 0.58 +0.2
47,605 - NIAGMN_28710 0.58 +0.0
47,629 - NIAGMN_28710 0.65 -0.3
47,629 - NIAGMN_28710 0.65 -0.0
47,629 - NIAGMN_28710 0.65 -0.8
47,673 + NIAGMN_28710 0.78 +2.3
47,770 - -0.2
47,960 - NIAGMN_28715 0.83 -0.5
48,045 + +2.6
48,152 + yncB NIAGMN_28720 0.24 +0.0
48,153 - yncB NIAGMN_28720 0.24 -0.8
48,161 + yncB NIAGMN_28720 0.26 -1.0
48,161 + yncB NIAGMN_28720 0.26 +0.4
48,162 - yncB NIAGMN_28720 0.26 +1.5
48,163 + yncB NIAGMN_28720 0.27 +1.6
48,164 - yncB NIAGMN_28720 0.27 +1.1
48,164 - yncB NIAGMN_28720 0.27 +3.6

Or see this region's nucleotide sequence