Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22020

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiM and NIAGMN_22020 overlap by 4 nucleotidesNIAGMN_22020 and yegX are separated by 64 nucleotidesyegX and yegW are separated by 51 nucleotides NIAGMN_22015: thiM - hydroxyethylthiazole kinase, at 4,341,539 to 4,342,327 thiM NIAGMN_22020: NIAGMN_22020 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 4,342,324 to 4,343,124 _22020 NIAGMN_22025: yegX - Uncharacterized protein YegX, at 4,343,189 to 4,344,007 yegX NIAGMN_22030: yegW - Uncharacterized HTH-type transcriptional regulator YegW, at 4,344,059 to 4,344,805 yegW Position (kb) 4342 4343 4344Strain fitness (log2 ratio) -2 -1 0 1at 4341.449 kb on + strandat 4341.449 kb on + strandat 4341.538 kb on + strandat 4341.538 kb on + strandat 4341.538 kb on + strandat 4341.538 kb on + strandat 4341.582 kb on + strandat 4341.583 kb on - strandat 4341.655 kb on + strand, within thiMat 4342.064 kb on + strand, within thiMat 4342.064 kb on + strand, within thiMat 4342.434 kb on + strand, within NIAGMN_22020at 4342.434 kb on + strand, within NIAGMN_22020at 4342.434 kb on + strand, within NIAGMN_22020at 4342.437 kb on + strand, within NIAGMN_22020at 4342.697 kb on + strand, within NIAGMN_22020at 4342.709 kb on + strand, within NIAGMN_22020at 4342.727 kb on + strand, within NIAGMN_22020at 4342.730 kb on + strand, within NIAGMN_22020at 4342.844 kb on + strand, within NIAGMN_22020at 4342.962 kb on + strand, within NIAGMN_22020at 4343.021 kb on + strand, within NIAGMN_22020at 4343.132 kb on - strandat 4343.132 kb on - strandat 4343.134 kb on + strandat 4343.134 kb on + strandat 4343.134 kb on + strandat 4343.134 kb on + strandat 4343.134 kb on + strandat 4343.136 kb on + strandat 4343.136 kb on + strandat 4343.136 kb on + strandat 4343.233 kb on - strandat 4343.321 kb on + strand, within yegXat 4343.322 kb on - strand, within yegXat 4343.322 kb on - strand, within yegXat 4343.333 kb on + strand, within yegXat 4343.333 kb on + strand, within yegXat 4343.334 kb on - strand, within yegXat 4343.381 kb on + strand, within yegXat 4343.397 kb on + strand, within yegXat 4343.397 kb on + strand, within yegXat 4343.397 kb on + strand, within yegXat 4343.530 kb on + strand, within yegXat 4343.541 kb on + strand, within yegXat 4343.542 kb on - strand, within yegXat 4343.673 kb on + strand, within yegXat 4343.767 kb on + strand, within yegXat 4343.811 kb on - strand, within yegXat 4343.843 kb on + strand, within yegXat 4343.843 kb on + strand, within yegXat 4343.844 kb on - strand, within yegXat 4343.844 kb on - strand, within yegXat 4343.913 kb on + strand, within yegXat 4344.005 kb on + strandat 4344.022 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,341,449 + +0.4
4,341,449 + -0.4
4,341,538 + -0.3
4,341,538 + -0.2
4,341,538 + -0.7
4,341,538 + -0.3
4,341,582 + -0.9
4,341,583 - -0.3
4,341,655 + thiM NIAGMN_22015 0.15 -1.9
4,342,064 + thiM NIAGMN_22015 0.67 +0.4
4,342,064 + thiM NIAGMN_22015 0.67 +0.5
4,342,434 + NIAGMN_22020 0.14 -0.8
4,342,434 + NIAGMN_22020 0.14 -1.8
4,342,434 + NIAGMN_22020 0.14 -0.8
4,342,437 + NIAGMN_22020 0.14 -1.8
4,342,697 + NIAGMN_22020 0.47 -0.4
4,342,709 + NIAGMN_22020 0.48 -0.8
4,342,727 + NIAGMN_22020 0.50 -1.2
4,342,730 + NIAGMN_22020 0.51 +0.4
4,342,844 + NIAGMN_22020 0.65 -0.3
4,342,962 + NIAGMN_22020 0.80 -1.3
4,343,021 + NIAGMN_22020 0.87 -0.1
4,343,132 - +0.4
4,343,132 - +1.7
4,343,134 + -0.1
4,343,134 + +0.4
4,343,134 + -0.2
4,343,134 + +0.5
4,343,134 + +0.5
4,343,136 + -0.1
4,343,136 + -0.3
4,343,136 + -0.8
4,343,233 - -0.8
4,343,321 + yegX NIAGMN_22025 0.16 -0.7
4,343,322 - yegX NIAGMN_22025 0.16 -0.1
4,343,322 - yegX NIAGMN_22025 0.16 +0.3
4,343,333 + yegX NIAGMN_22025 0.18 +0.9
4,343,333 + yegX NIAGMN_22025 0.18 -1.0
4,343,334 - yegX NIAGMN_22025 0.18 -0.9
4,343,381 + yegX NIAGMN_22025 0.23 -0.1
4,343,397 + yegX NIAGMN_22025 0.25 +0.8
4,343,397 + yegX NIAGMN_22025 0.25 -0.8
4,343,397 + yegX NIAGMN_22025 0.25 +0.2
4,343,530 + yegX NIAGMN_22025 0.42 -0.7
4,343,541 + yegX NIAGMN_22025 0.43 -2.0
4,343,542 - yegX NIAGMN_22025 0.43 +0.4
4,343,673 + yegX NIAGMN_22025 0.59 +0.0
4,343,767 + yegX NIAGMN_22025 0.71 -0.5
4,343,811 - yegX NIAGMN_22025 0.76 -0.6
4,343,843 + yegX NIAGMN_22025 0.80 -0.6
4,343,843 + yegX NIAGMN_22025 0.80 -0.1
4,343,844 - yegX NIAGMN_22025 0.80 +0.1
4,343,844 - yegX NIAGMN_22025 0.80 +1.5
4,343,913 + yegX NIAGMN_22025 0.88 +1.3
4,344,005 + +0.7
4,344,022 + +1.0

Or see this region's nucleotide sequence