Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt preA and preT overlap by 7 nucleotides preT and yeiS are separated by 193 nucleotides yeiS and sanA are separated by 2 nucleotides
NIAGMN_21315: preA - NAD-dependent dihydropyrimidine dehydrogenase subunit PreA, at 4,228,864 to 4,230,099
preA
NIAGMN_21320: preT - NAD-dependent dihydropyrimidine dehydrogenase subunit PreT, at 4,230,093 to 4,231,331
preT
NIAGMN_21325: yeiS - Uncharacterized protein YeiS, at 4,231,525 to 4,231,764
yeiS
NIAGMN_21330: sanA - outer membrane permeability protein SanA, at 4,231,767 to 4,232,486
sanA
Position (kb) 4230
4231
4232 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4229.103 kb on - strand, within preA at 4229.103 kb on - strand, within preA at 4229.103 kb on - strand, within preA at 4229.103 kb on - strand, within preA at 4229.133 kb on + strand, within preA at 4229.134 kb on - strand, within preA at 4229.153 kb on - strand, within preA at 4229.215 kb on - strand, within preA at 4229.215 kb on - strand, within preA at 4229.625 kb on - strand, within preA at 4230.012 kb on - strand at 4230.012 kb on - strand at 4230.230 kb on - strand, within preT at 4230.230 kb on - strand, within preT at 4230.252 kb on - strand, within preT at 4230.252 kb on - strand, within preT at 4230.352 kb on - strand, within preT at 4230.468 kb on + strand, within preT at 4230.558 kb on - strand, within preT at 4230.610 kb on - strand, within preT at 4230.610 kb on - strand, within preT at 4230.642 kb on - strand, within preT at 4230.759 kb on - strand, within preT at 4231.230 kb on - strand at 4231.360 kb on - strand at 4231.593 kb on + strand, within yeiS at 4231.594 kb on - strand, within yeiS at 4231.634 kb on + strand, within yeiS at 4231.756 kb on + strand at 4231.769 kb on - strand at 4232.026 kb on - strand, within sanA at 4232.265 kb on - strand, within sanA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,229,103 - preA NIAGMN_21315 0.19 -1.1 4,229,103 - preA NIAGMN_21315 0.19 -1.5 4,229,103 - preA NIAGMN_21315 0.19 +0.0 4,229,103 - preA NIAGMN_21315 0.19 -0.4 4,229,133 + preA NIAGMN_21315 0.22 -0.2 4,229,134 - preA NIAGMN_21315 0.22 +1.5 4,229,153 - preA NIAGMN_21315 0.23 +0.2 4,229,215 - preA NIAGMN_21315 0.28 -0.1 4,229,215 - preA NIAGMN_21315 0.28 +1.9 4,229,625 - preA NIAGMN_21315 0.62 +0.7 4,230,012 - -0.0 4,230,012 - -1.2 4,230,230 - preT NIAGMN_21320 0.11 -0.3 4,230,230 - preT NIAGMN_21320 0.11 -0.3 4,230,252 - preT NIAGMN_21320 0.13 -0.9 4,230,252 - preT NIAGMN_21320 0.13 -0.7 4,230,352 - preT NIAGMN_21320 0.21 -0.0 4,230,468 + preT NIAGMN_21320 0.30 -0.2 4,230,558 - preT NIAGMN_21320 0.38 +0.4 4,230,610 - preT NIAGMN_21320 0.42 +0.5 4,230,610 - preT NIAGMN_21320 0.42 +0.8 4,230,642 - preT NIAGMN_21320 0.44 +1.1 4,230,759 - preT NIAGMN_21320 0.54 +0.7 4,231,230 - +1.3 4,231,360 - +0.6 4,231,593 + yeiS NIAGMN_21325 0.28 -0.6 4,231,594 - yeiS NIAGMN_21325 0.29 -0.1 4,231,634 + yeiS NIAGMN_21325 0.45 +0.1 4,231,756 + +1.1 4,231,769 - +2.9 4,232,026 - sanA NIAGMN_21330 0.36 +0.2 4,232,265 - sanA NIAGMN_21330 0.69 -2.1
Or see this region's nucleotide sequence