Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20985

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntada and alkB overlap by 1 nucleotidesalkB and yojI are separated by 75 nucleotidesyojI and mqo are separated by 217 nucleotides NIAGMN_20975: ada - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 4,156,966 to 4,158,030 ada NIAGMN_20980: alkB - DNA oxidative demethylase AlkB, at 4,158,030 to 4,158,680 alkB NIAGMN_20985: yojI - microcin J25 efflux ABC transporter YojI, at 4,158,756 to 4,160,399 yojI NIAGMN_20990: mqo - malate dehydrogenase (quinone), at 4,160,617 to 4,162,263 mqo Position (kb) 4158 4159 4160 4161Strain fitness (log2 ratio) -2 -1 0 1at 4157.833 kb on + strand, within adaat 4157.919 kb on - strand, within adaat 4157.919 kb on - strand, within adaat 4158.028 kb on + strandat 4158.127 kb on + strand, within alkBat 4158.128 kb on - strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.194 kb on + strand, within alkBat 4158.195 kb on - strand, within alkBat 4158.195 kb on - strand, within alkBat 4158.195 kb on - strand, within alkBat 4158.195 kb on - strand, within alkBat 4158.195 kb on - strand, within alkBat 4158.255 kb on + strand, within alkBat 4158.513 kb on - strand, within alkBat 4158.692 kb on - strandat 4159.278 kb on - strand, within yojIat 4159.278 kb on - strand, within yojIat 4159.377 kb on + strand, within yojIat 4159.378 kb on - strand, within yojIat 4159.557 kb on + strand, within yojIat 4159.584 kb on - strand, within yojIat 4159.873 kb on - strand, within yojIat 4160.063 kb on + strand, within yojIat 4160.162 kb on + strand, within yojIat 4160.220 kb on + strand, within yojIat 4160.221 kb on - strand, within yojIat 4160.289 kb on + strandat 4160.289 kb on + strandat 4160.397 kb on + strandat 4160.398 kb on - strandat 4160.400 kb on + strandat 4160.446 kb on + strandat 4160.474 kb on - strandat 4160.611 kb on - strandat 4160.717 kb on + strandat 4160.717 kb on + strandat 4160.761 kb on + strandat 4160.762 kb on - strandat 4160.765 kb on + strandat 4161.019 kb on - strand, within mqoat 4161.019 kb on - strand, within mqoat 4161.261 kb on - strand, within mqoat 4161.285 kb on + strand, within mqo

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,157,833 + ada NIAGMN_20975 0.81 +0.5
4,157,919 - ada NIAGMN_20975 0.89 -0.0
4,157,919 - ada NIAGMN_20975 0.89 +1.1
4,158,028 + -2.2
4,158,127 + alkB NIAGMN_20980 0.15 -0.5
4,158,128 - alkB NIAGMN_20980 0.15 +0.0
4,158,194 + alkB NIAGMN_20980 0.25 +1.1
4,158,194 + alkB NIAGMN_20980 0.25 +0.3
4,158,194 + alkB NIAGMN_20980 0.25 -0.6
4,158,194 + alkB NIAGMN_20980 0.25 -0.0
4,158,194 + alkB NIAGMN_20980 0.25 -1.0
4,158,194 + alkB NIAGMN_20980 0.25 +0.2
4,158,194 + alkB NIAGMN_20980 0.25 -0.8
4,158,194 + alkB NIAGMN_20980 0.25 +1.2
4,158,194 + alkB NIAGMN_20980 0.25 -0.1
4,158,195 - alkB NIAGMN_20980 0.25 +0.7
4,158,195 - alkB NIAGMN_20980 0.25 -0.2
4,158,195 - alkB NIAGMN_20980 0.25 -1.5
4,158,195 - alkB NIAGMN_20980 0.25 -1.4
4,158,195 - alkB NIAGMN_20980 0.25 -0.4
4,158,255 + alkB NIAGMN_20980 0.35 +0.6
4,158,513 - alkB NIAGMN_20980 0.74 -1.9
4,158,692 - +0.5
4,159,278 - yojI NIAGMN_20985 0.32 -2.5
4,159,278 - yojI NIAGMN_20985 0.32 +0.7
4,159,377 + yojI NIAGMN_20985 0.38 -2.1
4,159,378 - yojI NIAGMN_20985 0.38 +0.3
4,159,557 + yojI NIAGMN_20985 0.49 +0.6
4,159,584 - yojI NIAGMN_20985 0.50 +1.0
4,159,873 - yojI NIAGMN_20985 0.68 +0.9
4,160,063 + yojI NIAGMN_20985 0.80 +0.0
4,160,162 + yojI NIAGMN_20985 0.86 -1.0
4,160,220 + yojI NIAGMN_20985 0.89 +1.3
4,160,221 - yojI NIAGMN_20985 0.89 -1.9
4,160,289 + +0.6
4,160,289 + +0.0
4,160,397 + -0.2
4,160,398 - +1.6
4,160,400 + -0.4
4,160,446 + -0.7
4,160,474 - -1.8
4,160,611 - -0.0
4,160,717 + -0.1
4,160,717 + +0.4
4,160,761 + -0.7
4,160,762 - -0.5
4,160,765 + -0.6
4,161,019 - mqo NIAGMN_20990 0.24 +0.2
4,161,019 - mqo NIAGMN_20990 0.24 -0.0
4,161,261 - mqo NIAGMN_20990 0.39 +0.6
4,161,285 + mqo NIAGMN_20990 0.41 +0.2

Or see this region's nucleotide sequence