Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01675

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntholE and yobB are separated by 101 nucleotidesyobB and exoX are separated by 23 nucleotidesexoX and ptrB overlap by 4 nucleotides NIAGMN_01665: holE - DNA polymerase III subunit theta, at 302,521 to 302,751 holE NIAGMN_01670: yobB - Uncharacterized protein YobB, at 302,853 to 303,509 yobB NIAGMN_01675: exoX - exodeoxyribonuclease X, at 303,533 to 304,195 exoX NIAGMN_01680: ptrB - oligopeptidase B, at 304,192 to 306,252 ptrB Position (kb) 303 304 305Strain fitness (log2 ratio) -1 0 1at 302.612 kb on - strand, within holEat 302.616 kb on + strand, within holEat 302.617 kb on - strand, within holEat 302.617 kb on - strand, within holEat 302.617 kb on - strand, within holEat 303.178 kb on - strand, within yobBat 303.454 kb on + strandat 303.658 kb on + strand, within exoXat 303.743 kb on + strand, within exoXat 303.885 kb on - strand, within exoXat 303.906 kb on + strand, within exoXat 303.953 kb on + strand, within exoXat 303.956 kb on - strand, within exoXat 304.193 kb on + strandat 304.268 kb on + strandat 304.318 kb on - strandat 304.388 kb on + strandat 304.479 kb on + strand, within ptrBat 304.705 kb on + strand, within ptrBat 304.743 kb on + strand, within ptrBat 304.823 kb on + strand, within ptrBat 304.858 kb on - strand, within ptrBat 304.890 kb on - strand, within ptrBat 305.051 kb on - strand, within ptrB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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302,612 - holE NIAGMN_01665 0.39 +1.3
302,616 + holE NIAGMN_01665 0.41 +0.5
302,617 - holE NIAGMN_01665 0.42 +0.3
302,617 - holE NIAGMN_01665 0.42 -0.2
302,617 - holE NIAGMN_01665 0.42 +0.5
303,178 - yobB NIAGMN_01670 0.49 -0.4
303,454 + +0.0
303,658 + exoX NIAGMN_01675 0.19 +0.4
303,743 + exoX NIAGMN_01675 0.32 +0.4
303,885 - exoX NIAGMN_01675 0.53 +0.2
303,906 + exoX NIAGMN_01675 0.56 -1.5
303,953 + exoX NIAGMN_01675 0.63 +0.2
303,956 - exoX NIAGMN_01675 0.64 -0.0
304,193 + +1.1
304,268 + +0.6
304,318 - -0.6
304,388 + +0.4
304,479 + ptrB NIAGMN_01680 0.14 -0.8
304,705 + ptrB NIAGMN_01680 0.25 -0.4
304,743 + ptrB NIAGMN_01680 0.27 +0.7
304,823 + ptrB NIAGMN_01680 0.31 -0.5
304,858 - ptrB NIAGMN_01680 0.32 +0.1
304,890 - ptrB NIAGMN_01680 0.34 -0.3
305,051 - ptrB NIAGMN_01680 0.42 -0.6

Or see this region's nucleotide sequence