Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01530

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyoaE and manX are separated by 462 nucleotidesmanX and manY are separated by 62 nucleotidesmanY and manZ are separated by 12 nucleotides NIAGMN_01525: yoaE - CNNM family cation transport protein YoaE, at 277,440 to 278,996 yoaE NIAGMN_01530: manX - PTS mannose transporter subunit IIAB, at 279,459 to 280,430 manX NIAGMN_01535: manY - PTS mannose transporter subunit IIC, at 280,493 to 281,293 manY NIAGMN_01540: manZ - PTS mannose transporter subunit IID, at 281,306 to 282,157 manZ Position (kb) 279 280 281Strain fitness (log2 ratio) -3 -2 -1 0 1at 278.730 kb on - strand, within yoaEat 279.019 kb on - strandat 279.369 kb on - strandat 279.441 kb on + strandat 279.453 kb on + strandat 279.471 kb on - strandat 279.478 kb on + strandat 279.478 kb on + strandat 279.532 kb on + strandat 279.659 kb on + strand, within manXat 279.659 kb on + strand, within manXat 279.659 kb on + strand, within manXat 279.659 kb on + strand, within manXat 279.659 kb on + strand, within manXat 279.940 kb on - strand, within manXat 280.031 kb on + strand, within manXat 280.070 kb on + strand, within manXat 280.118 kb on - strand, within manXat 280.186 kb on + strand, within manXat 280.466 kb on + strandat 281.357 kb on - strandat 281.380 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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278,730 - yoaE NIAGMN_01525 0.83 -2.1
279,019 - +0.3
279,369 - +0.2
279,441 + -0.8
279,453 + +1.3
279,471 - +0.3
279,478 + +1.3
279,478 + -0.1
279,532 + +0.8
279,659 + manX NIAGMN_01530 0.21 -1.7
279,659 + manX NIAGMN_01530 0.21 -0.7
279,659 + manX NIAGMN_01530 0.21 +1.7
279,659 + manX NIAGMN_01530 0.21 +0.7
279,659 + manX NIAGMN_01530 0.21 -1.4
279,940 - manX NIAGMN_01530 0.49 +1.6
280,031 + manX NIAGMN_01530 0.59 -1.1
280,070 + manX NIAGMN_01530 0.63 +0.1
280,118 - manX NIAGMN_01530 0.68 -0.2
280,186 + manX NIAGMN_01530 0.75 -2.9
280,466 + -2.0
281,357 - -2.1
281,380 + +1.3

Or see this region's nucleotide sequence