Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01025

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_01015 and yniD are separated by 174 nucleotidesyniD and ydiY are separated by 52 nucleotidesydiY and pfkB are separated by 286 nucleotides NIAGMN_01015: NIAGMN_01015 - ShET2 enterotoxin, N-terminal region, at 178,377 to 180,275 _01015 NIAGMN_01020: yniD - Uncharacterized protein YniD, at 180,450 to 180,557 yniD NIAGMN_01025: ydiY - Uncharacterized protein YdiY, at 180,610 to 181,368 ydiY NIAGMN_01030: pfkB - 6-phosphofructokinase II, at 181,655 to 182,584 pfkB Position (kb) 180 181 182Strain fitness (log2 ratio) -3 -2 -1 0 1at 180.459 kb on - strandat 180.632 kb on - strandat 180.632 kb on - strandat 180.652 kb on - strandat 180.729 kb on + strand, within ydiYat 180.730 kb on - strand, within ydiYat 180.831 kb on - strand, within ydiYat 180.846 kb on - strand, within ydiYat 180.880 kb on - strand, within ydiYat 180.882 kb on - strand, within ydiYat 181.111 kb on - strand, within ydiYat 181.197 kb on + strand, within ydiYat 181.492 kb on + strandat 181.553 kb on + strandat 181.553 kb on + strandat 181.663 kb on + strandat 181.664 kb on - strandat 181.697 kb on - strandat 181.938 kb on + strand, within pfkBat 181.938 kb on + strand, within pfkBat 181.938 kb on + strand, within pfkBat 182.207 kb on - strand, within pfkBat 182.296 kb on + strand, within pfkBat 182.296 kb on + strand, within pfkB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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180,459 - -0.6
180,632 - +0.6
180,632 - +0.9
180,652 - -0.2
180,729 + ydiY NIAGMN_01025 0.16 +0.6
180,730 - ydiY NIAGMN_01025 0.16 +0.2
180,831 - ydiY NIAGMN_01025 0.29 -1.1
180,846 - ydiY NIAGMN_01025 0.31 +0.4
180,880 - ydiY NIAGMN_01025 0.36 -0.1
180,882 - ydiY NIAGMN_01025 0.36 -0.2
181,111 - ydiY NIAGMN_01025 0.66 -0.3
181,197 + ydiY NIAGMN_01025 0.77 -0.3
181,492 + +0.1
181,553 + -0.9
181,553 + -1.2
181,663 + +0.4
181,664 - -0.9
181,697 - -3.6
181,938 + pfkB NIAGMN_01030 0.30 -2.8
181,938 + pfkB NIAGMN_01030 0.30 +1.1
181,938 + pfkB NIAGMN_01030 0.30 -1.2
182,207 - pfkB NIAGMN_01030 0.59 -0.2
182,296 + pfkB NIAGMN_01030 0.69 +0.1
182,296 + pfkB NIAGMN_01030 0.69 -1.5

Or see this region's nucleotide sequence