Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00915

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiS and ydiT overlap by 4 nucleotidesydiT and fadK are separated by 56 nucleotidesfadK and ppsA are separated by 56 nucleotides NIAGMN_00905: ydiS - putative electron transfer flavoprotein-quinone oxidoreductase YdiS, at 156,680 to 157,969 ydiS NIAGMN_00910: ydiT - Ferredoxin-like protein YdiT, at 157,966 to 158,259 ydiT NIAGMN_00915: fadK - medium-chain fatty-acid--CoA ligase, at 158,316 to 159,962 fadK NIAGMN_00920: ppsA - phosphoenolpyruvate synthase, at 160,019 to 162,397 ppsA Position (kb) 158 159 160Strain fitness (log2 ratio) -1 0 1at 157.516 kb on + strand, within ydiSat 157.868 kb on - strandat 158.113 kb on + strand, within ydiTat 158.467 kb on - strandat 158.673 kb on - strand, within fadKat 158.767 kb on + strand, within fadKat 159.097 kb on + strand, within fadKat 159.985 kb on - strandat 160.159 kb on - strandat 160.560 kb on + strand, within ppsAat 160.561 kb on - strand, within ppsAat 160.611 kb on + strand, within ppsA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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157,516 + ydiS NIAGMN_00905 0.65 -1.3
157,868 - +0.2
158,113 + ydiT NIAGMN_00910 0.50 +0.1
158,467 - -0.2
158,673 - fadK NIAGMN_00915 0.22 +0.1
158,767 + fadK NIAGMN_00915 0.27 +1.4
159,097 + fadK NIAGMN_00915 0.47 +0.1
159,985 - +1.3
160,159 - -1.3
160,560 + ppsA NIAGMN_00920 0.23 -0.7
160,561 - ppsA NIAGMN_00920 0.23 +0.5
160,611 + ppsA NIAGMN_00920 0.25 +0.9

Or see this region's nucleotide sequence