Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00335

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclcB and bioD overlap by 48 nucleotidesbioD and mlc are separated by 124 nucleotidesmlc and ynfL are separated by 134 nucleotides NIAGMN_00325: clcB - voltage-gated ClC-type chloride channel ClcB, at 43,394 to 44,650 clcB NIAGMN_00330: bioD - dethiobiotin synthase, at 44,603 to 45,298 bioD NIAGMN_00335: mlc - DNA-binding transcriptional repressor Mlc, at 45,423 to 46,643 mlc NIAGMN_00340: ynfL - Uncharacterized HTH-type transcriptional regulator YnfL, at 46,778 to 47,671 ynfL Position (kb) 45 46 47Strain fitness (log2 ratio) -2 -1 0 1 2at 44.543 kb on + strandat 44.551 kb on - strandat 44.577 kb on + strandat 44.618 kb on - strandat 44.649 kb on - strandat 45.094 kb on - strand, within bioDat 45.187 kb on + strand, within bioDat 45.258 kb on - strandat 45.613 kb on - strand, within mlcat 46.126 kb on - strand, within mlcat 46.135 kb on + strand, within mlcat 46.152 kb on + strand, within mlcat 46.683 kb on - strandat 46.727 kb on + strandat 47.056 kb on - strand, within ynfLat 47.314 kb on - strand, within ynfLat 47.464 kb on + strand, within ynfL

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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44,543 + +0.5
44,551 - -0.7
44,577 + +0.3
44,618 - +0.2
44,649 - -1.5
45,094 - bioD NIAGMN_00330 0.71 -0.2
45,187 + bioD NIAGMN_00330 0.84 +0.3
45,258 - -0.3
45,613 - mlc NIAGMN_00335 0.16 +0.5
46,126 - mlc NIAGMN_00335 0.58 -2.2
46,135 + mlc NIAGMN_00335 0.58 +0.7
46,152 + mlc NIAGMN_00335 0.60 +0.7
46,683 - +1.9
46,727 + -1.6
47,056 - ynfL NIAGMN_00340 0.31 +0.1
47,314 - ynfL NIAGMN_00340 0.60 +0.3
47,464 + ynfL NIAGMN_00340 0.77 -1.9

Or see this region's nucleotide sequence