Strain Fitness in Escherichia coli ECRC101 around MCAODC_25305

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgsiD and gsiC are separated by 2 nucleotidesgsiC and gsiB are separated by 17 nucleotides MCAODC_25300: gsiD - glutathione ABC transporter permease GsiD, at 1,783,570 to 1,784,481 gsiD MCAODC_25305: gsiC - glutathione ABC transporter permease GsiC, at 1,784,484 to 1,785,404 gsiC MCAODC_25310: gsiB - glutathione ABC transporter substrate-binding protein GsiB, at 1,785,422 to 1,786,960 gsiB Position (kb) 1784 1785 1786Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1783.578 kb on + strandat 1783.780 kb on - strand, within gsiDat 1783.818 kb on + strand, within gsiDat 1783.819 kb on - strand, within gsiDat 1783.842 kb on + strand, within gsiDat 1783.843 kb on - strand, within gsiDat 1783.843 kb on - strand, within gsiDat 1783.863 kb on + strand, within gsiDat 1783.977 kb on + strand, within gsiDat 1783.978 kb on - strand, within gsiDat 1783.986 kb on + strand, within gsiDat 1784.030 kb on - strand, within gsiDat 1784.097 kb on - strand, within gsiDat 1784.097 kb on - strand, within gsiDat 1784.100 kb on - strand, within gsiDat 1784.409 kb on + strandat 1784.409 kb on + strandat 1784.410 kb on - strandat 1784.410 kb on - strandat 1784.410 kb on - strandat 1784.413 kb on + strandat 1784.413 kb on + strandat 1784.413 kb on + strandat 1784.413 kb on + strandat 1784.414 kb on - strandat 1784.414 kb on - strandat 1784.414 kb on - strandat 1784.414 kb on - strandat 1784.485 kb on + strandat 1784.485 kb on + strandat 1784.485 kb on + strandat 1784.486 kb on - strandat 1784.486 kb on - strandat 1784.536 kb on - strandat 1784.694 kb on + strand, within gsiCat 1784.695 kb on - strand, within gsiCat 1784.733 kb on + strand, within gsiCat 1784.792 kb on + strand, within gsiCat 1784.793 kb on - strand, within gsiCat 1784.794 kb on + strand, within gsiCat 1784.795 kb on - strand, within gsiCat 1784.795 kb on - strand, within gsiCat 1784.887 kb on + strand, within gsiCat 1784.888 kb on - strand, within gsiCat 1784.915 kb on + strand, within gsiCat 1784.943 kb on + strand, within gsiCat 1784.953 kb on - strand, within gsiCat 1785.009 kb on - strand, within gsiCat 1785.097 kb on - strand, within gsiCat 1785.120 kb on - strand, within gsiCat 1785.200 kb on + strand, within gsiCat 1785.201 kb on - strand, within gsiCat 1785.203 kb on - strand, within gsiCat 1785.220 kb on + strand, within gsiCat 1785.220 kb on + strand, within gsiCat 1785.221 kb on - strand, within gsiCat 1785.261 kb on + strand, within gsiCat 1785.262 kb on - strand, within gsiCat 1785.262 kb on - strand, within gsiCat 1785.320 kb on + strandat 1785.321 kb on - strandat 1785.321 kb on - strandat 1785.394 kb on + strandat 1785.394 kb on + strandat 1785.395 kb on - strandat 1785.399 kb on - strandat 1785.399 kb on - strandat 1785.399 kb on - strandat 1785.399 kb on - strandat 1785.399 kb on - strandat 1785.399 kb on - strandat 1785.490 kb on + strandat 1785.491 kb on - strandat 1785.554 kb on - strandat 1785.554 kb on - strandat 1785.568 kb on + strandat 1785.569 kb on - strandat 1785.569 kb on - strandat 1785.569 kb on - strandat 1785.569 kb on - strandat 1785.569 kb on - strandat 1785.570 kb on + strandat 1785.570 kb on + strandat 1785.570 kb on + strandat 1785.570 kb on + strandat 1785.570 kb on + strandat 1785.570 kb on + strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.571 kb on - strandat 1785.572 kb on + strandat 1785.572 kb on + strandat 1785.573 kb on - strandat 1785.573 kb on - strandat 1785.598 kb on + strand, within gsiBat 1785.598 kb on + strand, within gsiBat 1785.599 kb on - strand, within gsiBat 1785.638 kb on - strand, within gsiBat 1785.656 kb on - strand, within gsiBat 1785.744 kb on + strand, within gsiBat 1785.745 kb on - strand, within gsiBat 1785.745 kb on - strand, within gsiBat 1785.778 kb on + strand, within gsiBat 1785.778 kb on + strand, within gsiBat 1785.779 kb on - strand, within gsiBat 1785.896 kb on - strand, within gsiBat 1785.951 kb on - strand, within gsiBat 1785.976 kb on - strand, within gsiBat 1785.989 kb on - strand, within gsiBat 1785.990 kb on + strand, within gsiBat 1786.030 kb on + strand, within gsiBat 1786.030 kb on + strand, within gsiBat 1786.044 kb on + strand, within gsiBat 1786.045 kb on - strand, within gsiBat 1786.082 kb on - strand, within gsiBat 1786.144 kb on + strand, within gsiBat 1786.150 kb on + strand, within gsiBat 1786.150 kb on + strand, within gsiBat 1786.151 kb on - strand, within gsiBat 1786.151 kb on - strand, within gsiBat 1786.151 kb on - strand, within gsiBat 1786.152 kb on + strand, within gsiBat 1786.152 kb on + strand, within gsiBat 1786.153 kb on - strand, within gsiBat 1786.153 kb on - strand, within gsiBat 1786.153 kb on - strand, within gsiBat 1786.163 kb on - strand, within gsiBat 1786.163 kb on - strand, within gsiBat 1786.163 kb on - strand, within gsiBat 1786.163 kb on - strand, within gsiBat 1786.307 kb on + strand, within gsiBat 1786.307 kb on + strand, within gsiBat 1786.307 kb on + strand, within gsiBat 1786.307 kb on + strand, within gsiBat 1786.307 kb on + strand, within gsiBat 1786.308 kb on - strand, within gsiBat 1786.308 kb on - strand, within gsiBat 1786.399 kb on - strand, within gsiBat 1786.399 kb on - strand, within gsiB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,783,578 + -1.0
1,783,780 - gsiD MCAODC_25300 0.23 +1.2
1,783,818 + gsiD MCAODC_25300 0.27 -1.5
1,783,819 - gsiD MCAODC_25300 0.27 +1.1
1,783,842 + gsiD MCAODC_25300 0.30 +2.0
1,783,843 - gsiD MCAODC_25300 0.30 +1.2
1,783,843 - gsiD MCAODC_25300 0.30 -0.1
1,783,863 + gsiD MCAODC_25300 0.32 +0.0
1,783,977 + gsiD MCAODC_25300 0.45 +0.1
1,783,978 - gsiD MCAODC_25300 0.45 -2.4
1,783,986 + gsiD MCAODC_25300 0.46 -0.4
1,784,030 - gsiD MCAODC_25300 0.50 +0.3
1,784,097 - gsiD MCAODC_25300 0.58 +1.1
1,784,097 - gsiD MCAODC_25300 0.58 -0.3
1,784,100 - gsiD MCAODC_25300 0.58 -1.0
1,784,409 + +0.4
1,784,409 + +1.0
1,784,410 - -0.0
1,784,410 - -0.2
1,784,410 - -2.5
1,784,413 + +0.6
1,784,413 + +0.5
1,784,413 + +0.1
1,784,413 + +0.0
1,784,414 - -2.4
1,784,414 - +1.0
1,784,414 - -0.3
1,784,414 - +0.3
1,784,485 + -0.3
1,784,485 + -0.9
1,784,485 + +0.7
1,784,486 - +0.0
1,784,486 - -0.4
1,784,536 - +0.3
1,784,694 + gsiC MCAODC_25305 0.23 -1.4
1,784,695 - gsiC MCAODC_25305 0.23 +0.4
1,784,733 + gsiC MCAODC_25305 0.27 +0.0
1,784,792 + gsiC MCAODC_25305 0.33 +0.3
1,784,793 - gsiC MCAODC_25305 0.34 -1.2
1,784,794 + gsiC MCAODC_25305 0.34 -1.7
1,784,795 - gsiC MCAODC_25305 0.34 -0.4
1,784,795 - gsiC MCAODC_25305 0.34 -0.9
1,784,887 + gsiC MCAODC_25305 0.44 -0.2
1,784,888 - gsiC MCAODC_25305 0.44 -1.2
1,784,915 + gsiC MCAODC_25305 0.47 -2.7
1,784,943 + gsiC MCAODC_25305 0.50 -2.3
1,784,953 - gsiC MCAODC_25305 0.51 -0.8
1,785,009 - gsiC MCAODC_25305 0.57 -1.0
1,785,097 - gsiC MCAODC_25305 0.67 +1.6
1,785,120 - gsiC MCAODC_25305 0.69 -2.2
1,785,200 + gsiC MCAODC_25305 0.78 +0.3
1,785,201 - gsiC MCAODC_25305 0.78 -1.2
1,785,203 - gsiC MCAODC_25305 0.78 +0.4
1,785,220 + gsiC MCAODC_25305 0.80 +0.4
1,785,220 + gsiC MCAODC_25305 0.80 -1.5
1,785,221 - gsiC MCAODC_25305 0.80 -0.3
1,785,261 + gsiC MCAODC_25305 0.84 +1.3
1,785,262 - gsiC MCAODC_25305 0.84 -4.0
1,785,262 - gsiC MCAODC_25305 0.84 -0.3
1,785,320 + -0.1
1,785,321 - -0.3
1,785,321 - +0.6
1,785,394 + +1.0
1,785,394 + -1.0
1,785,395 - +0.0
1,785,399 - +0.4
1,785,399 - -0.2
1,785,399 - -1.7
1,785,399 - +0.6
1,785,399 - -1.4
1,785,399 - +1.0
1,785,490 + -0.5
1,785,491 - +0.0
1,785,554 - +0.8
1,785,554 - -0.0
1,785,568 + +0.8
1,785,569 - +0.7
1,785,569 - -1.2
1,785,569 - -2.1
1,785,569 - -0.6
1,785,569 - +0.0
1,785,570 + -0.1
1,785,570 + -0.3
1,785,570 + -0.1
1,785,570 + +1.8
1,785,570 + +0.3
1,785,570 + +0.5
1,785,571 - -0.2
1,785,571 - -1.5
1,785,571 - -0.2
1,785,571 - -0.9
1,785,571 - -0.8
1,785,571 - +0.9
1,785,571 - -0.4
1,785,572 + +0.3
1,785,572 + -0.1
1,785,573 - +0.9
1,785,573 - +0.2
1,785,598 + gsiB MCAODC_25310 0.11 -0.0
1,785,598 + gsiB MCAODC_25310 0.11 +0.1
1,785,599 - gsiB MCAODC_25310 0.12 +0.5
1,785,638 - gsiB MCAODC_25310 0.14 +0.1
1,785,656 - gsiB MCAODC_25310 0.15 -0.4
1,785,744 + gsiB MCAODC_25310 0.21 +0.5
1,785,745 - gsiB MCAODC_25310 0.21 +0.0
1,785,745 - gsiB MCAODC_25310 0.21 -0.7
1,785,778 + gsiB MCAODC_25310 0.23 +0.5
1,785,778 + gsiB MCAODC_25310 0.23 -0.7
1,785,779 - gsiB MCAODC_25310 0.23 +0.5
1,785,896 - gsiB MCAODC_25310 0.31 -0.1
1,785,951 - gsiB MCAODC_25310 0.34 +0.9
1,785,976 - gsiB MCAODC_25310 0.36 -0.2
1,785,989 - gsiB MCAODC_25310 0.37 +0.3
1,785,990 + gsiB MCAODC_25310 0.37 -0.2
1,786,030 + gsiB MCAODC_25310 0.40 -1.5
1,786,030 + gsiB MCAODC_25310 0.40 +0.0
1,786,044 + gsiB MCAODC_25310 0.40 -2.0
1,786,045 - gsiB MCAODC_25310 0.40 +0.3
1,786,082 - gsiB MCAODC_25310 0.43 -1.2
1,786,144 + gsiB MCAODC_25310 0.47 -3.3
1,786,150 + gsiB MCAODC_25310 0.47 +0.8
1,786,150 + gsiB MCAODC_25310 0.47 -1.3
1,786,151 - gsiB MCAODC_25310 0.47 +1.2
1,786,151 - gsiB MCAODC_25310 0.47 -0.2
1,786,151 - gsiB MCAODC_25310 0.47 -0.6
1,786,152 + gsiB MCAODC_25310 0.47 -0.3
1,786,152 + gsiB MCAODC_25310 0.47 +1.8
1,786,153 - gsiB MCAODC_25310 0.47 +0.6
1,786,153 - gsiB MCAODC_25310 0.47 +0.2
1,786,153 - gsiB MCAODC_25310 0.47 +0.8
1,786,163 - gsiB MCAODC_25310 0.48 -1.0
1,786,163 - gsiB MCAODC_25310 0.48 -0.1
1,786,163 - gsiB MCAODC_25310 0.48 -0.2
1,786,163 - gsiB MCAODC_25310 0.48 -1.5
1,786,307 + gsiB MCAODC_25310 0.58 +1.0
1,786,307 + gsiB MCAODC_25310 0.58 +0.1
1,786,307 + gsiB MCAODC_25310 0.58 +0.1
1,786,307 + gsiB MCAODC_25310 0.58 -0.4
1,786,307 + gsiB MCAODC_25310 0.58 -0.9
1,786,308 - gsiB MCAODC_25310 0.58 +0.4
1,786,308 - gsiB MCAODC_25310 0.58 -0.4
1,786,399 - gsiB MCAODC_25310 0.63 +0.3
1,786,399 - gsiB MCAODC_25310 0.63 -2.4

Or see this region's nucleotide sequence