Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt shiA and amn are separated by 101 nucleotides amn and ycdB are separated by 342 nucleotides
MCAODC_20205: shiA - shikimate transporter, at 852,321 to 853,637
shiA
MCAODC_20210: amn - AMP nucleosidase, at 853,739 to 855,193
amn
MCAODC_20215: ycdB - putative transcriptional regulatory protein YcdB, at 855,536 to 856,252
ycdB
Position (kb) 853
854
855
856 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 852.805 kb on - strand, within shiA at 852.835 kb on + strand, within shiA at 852.933 kb on - strand, within shiA at 853.010 kb on + strand, within shiA at 853.010 kb on + strand, within shiA at 853.120 kb on - strand, within shiA at 853.190 kb on + strand, within shiA at 853.276 kb on - strand, within shiA at 853.278 kb on - strand, within shiA at 853.453 kb on - strand, within shiA at 853.455 kb on - strand, within shiA at 853.505 kb on + strand, within shiA at 853.514 kb on + strand at 853.536 kb on + strand at 853.536 kb on + strand at 853.557 kb on - strand at 853.570 kb on - strand at 853.669 kb on + strand at 853.698 kb on - strand at 853.700 kb on + strand at 853.715 kb on - strand at 853.720 kb on + strand at 853.744 kb on + strand at 853.747 kb on + strand at 853.747 kb on + strand at 853.748 kb on - strand at 853.748 kb on - strand at 853.842 kb on - strand at 853.847 kb on - strand at 853.970 kb on - strand, within amn at 853.970 kb on - strand, within amn at 854.030 kb on - strand, within amn at 854.041 kb on + strand, within amn at 854.042 kb on - strand, within amn at 854.071 kb on + strand, within amn at 854.111 kb on + strand, within amn at 854.111 kb on + strand, within amn at 854.111 kb on + strand, within amn at 854.112 kb on - strand, within amn at 854.112 kb on - strand, within amn at 854.112 kb on - strand, within amn at 854.164 kb on - strand, within amn at 854.171 kb on + strand, within amn at 854.225 kb on + strand, within amn at 854.304 kb on - strand, within amn at 854.306 kb on - strand, within amn at 854.354 kb on + strand, within amn at 854.355 kb on - strand, within amn at 854.364 kb on - strand, within amn at 854.414 kb on - strand, within amn at 854.554 kb on + strand, within amn at 854.555 kb on - strand, within amn at 854.557 kb on + strand, within amn at 854.557 kb on + strand, within amn at 854.557 kb on + strand, within amn at 854.570 kb on - strand, within amn at 854.593 kb on + strand, within amn at 854.622 kb on + strand, within amn at 854.752 kb on + strand, within amn at 854.852 kb on - strand, within amn at 854.858 kb on - strand, within amn at 854.892 kb on + strand, within amn at 855.023 kb on - strand, within amn at 855.172 kb on + strand at 855.191 kb on + strand at 855.194 kb on + strand at 855.254 kb on + strand at 856.042 kb on + strand, within ycdB at 856.046 kb on + strand, within ycdB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 852,805 - shiA MCAODC_20205 0.37 -0.4 852,835 + shiA MCAODC_20205 0.39 -0.5 852,933 - shiA MCAODC_20205 0.46 -0.3 853,010 + shiA MCAODC_20205 0.52 -2.4 853,010 + shiA MCAODC_20205 0.52 -3.3 853,120 - shiA MCAODC_20205 0.61 +0.1 853,190 + shiA MCAODC_20205 0.66 -0.5 853,276 - shiA MCAODC_20205 0.73 +1.0 853,278 - shiA MCAODC_20205 0.73 +0.4 853,453 - shiA MCAODC_20205 0.86 +3.2 853,455 - shiA MCAODC_20205 0.86 +1.3 853,505 + shiA MCAODC_20205 0.90 -0.7 853,514 + +0.6 853,536 + +1.5 853,536 + +0.5 853,557 - -1.0 853,570 - -0.4 853,669 + -0.5 853,698 - -5.1 853,700 + +0.1 853,715 - -0.7 853,720 + -0.4 853,744 + -0.2 853,747 + -1.0 853,747 + -1.1 853,748 - +1.9 853,748 - +0.3 853,842 - -1.0 853,847 - -0.8 853,970 - amn MCAODC_20210 0.16 -0.1 853,970 - amn MCAODC_20210 0.16 +0.3 854,030 - amn MCAODC_20210 0.20 +0.9 854,041 + amn MCAODC_20210 0.21 -1.7 854,042 - amn MCAODC_20210 0.21 -0.1 854,071 + amn MCAODC_20210 0.23 -3.0 854,111 + amn MCAODC_20210 0.26 -2.5 854,111 + amn MCAODC_20210 0.26 -0.3 854,111 + amn MCAODC_20210 0.26 -3.9 854,112 - amn MCAODC_20210 0.26 -0.3 854,112 - amn MCAODC_20210 0.26 +0.3 854,112 - amn MCAODC_20210 0.26 -0.2 854,164 - amn MCAODC_20210 0.29 +0.3 854,171 + amn MCAODC_20210 0.30 -1.8 854,225 + amn MCAODC_20210 0.33 -1.0 854,304 - amn MCAODC_20210 0.39 +0.3 854,306 - amn MCAODC_20210 0.39 -2.2 854,354 + amn MCAODC_20210 0.42 +0.5 854,355 - amn MCAODC_20210 0.42 +1.3 854,364 - amn MCAODC_20210 0.43 +0.3 854,414 - amn MCAODC_20210 0.46 +1.9 854,554 + amn MCAODC_20210 0.56 -1.6 854,555 - amn MCAODC_20210 0.56 +0.7 854,557 + amn MCAODC_20210 0.56 -2.4 854,557 + amn MCAODC_20210 0.56 -1.3 854,557 + amn MCAODC_20210 0.56 +0.4 854,570 - amn MCAODC_20210 0.57 +1.0 854,593 + amn MCAODC_20210 0.59 +1.9 854,622 + amn MCAODC_20210 0.61 -0.0 854,752 + amn MCAODC_20210 0.70 -1.5 854,852 - amn MCAODC_20210 0.76 +0.3 854,858 - amn MCAODC_20210 0.77 -2.3 854,892 + amn MCAODC_20210 0.79 +1.8 855,023 - amn MCAODC_20210 0.88 +2.7 855,172 + +1.1 855,191 + -0.5 855,194 + -1.7 855,254 + +0.9 856,042 + ycdB MCAODC_20215 0.71 +1.6 856,046 + ycdB MCAODC_20215 0.71 +1.2
Or see this region's nucleotide sequence