Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides fliR and rcsA are separated by 289 nucleotides rcsA and dsrB are separated by 43 nucleotides
MCAODC_19730: fliP - flagellar type III secretion system pore protein FliP, at 775,624 to 776,361
fliP
MCAODC_19735: fliQ - flagellar biosynthesis protein FliQ, at 776,371 to 776,640
fliQ
MCAODC_19740: fliR - flagellar type III secretion system protein FliR, at 776,648 to 777,433
fliR
MCAODC_19745: rcsA - transcriptional regulator RcsA, at 777,723 to 778,346
rcsA
MCAODC_19750: dsrB - protein DsrB, at 778,390 to 778,578
dsrB
Position (kb) 776
777
778 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 775.668 kb on + strand at 775.879 kb on - strand, within fliP at 775.879 kb on - strand, within fliP at 775.920 kb on + strand, within fliP at 775.923 kb on + strand, within fliP at 775.986 kb on - strand, within fliP at 776.222 kb on + strand, within fliP at 776.353 kb on + strand at 776.359 kb on + strand at 776.557 kb on - strand, within fliQ at 776.627 kb on - strand at 776.627 kb on - strand at 776.629 kb on - strand at 776.644 kb on + strand at 776.645 kb on - strand at 776.647 kb on + strand at 776.647 kb on + strand at 776.647 kb on + strand at 776.647 kb on + strand at 776.647 kb on + strand at 776.648 kb on - strand at 776.648 kb on - strand at 776.648 kb on - strand at 776.648 kb on - strand at 776.648 kb on - strand at 776.648 kb on - strand at 776.694 kb on - strand at 776.821 kb on - strand, within fliR at 776.821 kb on - strand, within fliR at 777.007 kb on + strand, within fliR at 777.007 kb on + strand, within fliR at 777.007 kb on + strand, within fliR at 777.008 kb on - strand, within fliR at 777.008 kb on - strand, within fliR at 777.008 kb on - strand, within fliR at 777.008 kb on - strand, within fliR at 777.011 kb on - strand, within fliR at 777.038 kb on - strand, within fliR at 777.038 kb on - strand, within fliR at 777.038 kb on - strand, within fliR at 777.068 kb on - strand, within fliR at 777.078 kb on + strand, within fliR at 777.250 kb on - strand, within fliR at 777.256 kb on - strand, within fliR at 777.605 kb on + strand at 777.695 kb on - strand at 777.695 kb on - strand at 777.764 kb on - strand at 777.777 kb on + strand at 778.038 kb on - strand, within rcsA at 778.115 kb on - strand, within rcsA at 778.244 kb on + strand, within rcsA at 778.257 kb on - strand, within rcsA at 778.287 kb on + strand at 778.413 kb on + strand, within dsrB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 775,668 + +0.6 775,879 - fliP MCAODC_19730 0.35 -0.4 775,879 - fliP MCAODC_19730 0.35 -0.3 775,920 + fliP MCAODC_19730 0.40 -0.1 775,923 + fliP MCAODC_19730 0.41 -0.2 775,986 - fliP MCAODC_19730 0.49 +0.4 776,222 + fliP MCAODC_19730 0.81 +0.5 776,353 + +0.3 776,359 + -1.1 776,557 - fliQ MCAODC_19735 0.69 +0.6 776,627 - -0.2 776,627 - +0.6 776,629 - +0.3 776,644 + -2.1 776,645 - -1.6 776,647 + +0.9 776,647 + +1.1 776,647 + +0.5 776,647 + -0.6 776,647 + -2.1 776,648 - -1.0 776,648 - -2.2 776,648 - +2.3 776,648 - -0.3 776,648 - +0.3 776,648 - -0.2 776,694 - -3.5 776,821 - fliR MCAODC_19740 0.22 -0.5 776,821 - fliR MCAODC_19740 0.22 -0.2 777,007 + fliR MCAODC_19740 0.46 -1.6 777,007 + fliR MCAODC_19740 0.46 -0.2 777,007 + fliR MCAODC_19740 0.46 +1.5 777,008 - fliR MCAODC_19740 0.46 -0.2 777,008 - fliR MCAODC_19740 0.46 +0.1 777,008 - fliR MCAODC_19740 0.46 -1.3 777,008 - fliR MCAODC_19740 0.46 -0.1 777,011 - fliR MCAODC_19740 0.46 +0.5 777,038 - fliR MCAODC_19740 0.50 +0.2 777,038 - fliR MCAODC_19740 0.50 +0.9 777,038 - fliR MCAODC_19740 0.50 -2.0 777,068 - fliR MCAODC_19740 0.53 +1.2 777,078 + fliR MCAODC_19740 0.55 +0.6 777,250 - fliR MCAODC_19740 0.77 -2.8 777,256 - fliR MCAODC_19740 0.77 +0.5 777,605 + -1.6 777,695 - +1.3 777,695 - -2.9 777,764 - +0.3 777,777 + -1.7 778,038 - rcsA MCAODC_19745 0.50 -0.6 778,115 - rcsA MCAODC_19745 0.63 -0.7 778,244 + rcsA MCAODC_19745 0.83 +0.7 778,257 - rcsA MCAODC_19745 0.86 -1.1 778,287 + -1.1 778,413 + dsrB MCAODC_19750 0.12 -1.4
Or see this region's nucleotide sequence