Strain Fitness in Escherichia coli ECRC101 around MCAODC_14510

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpsuG and psuT are separated by 93 nucleotidespsuT and yeiL are separated by 70 nucleotidesyeiL and rihB are separated by 129 nucleotides MCAODC_14505: psuG - pseudouridine-5'-phosphate glycosidase, at 2,854,425 to 2,855,363 psuG MCAODC_14510: psuT - Putative pseudouridine transporter, at 2,855,457 to 2,856,707 psuT MCAODC_14515: yeiL - Regulatory protein YeiL, at 2,856,778 to 2,857,476 yeiL MCAODC_14520: rihB - ribosylpyrimidine nucleosidase, at 2,857,606 to 2,858,547 rihB Position (kb) 2855 2856 2857Strain fitness (log2 ratio) -2 -1 0 1 2at 2854.804 kb on + strand, within psuGat 2855.166 kb on - strand, within psuGat 2855.166 kb on - strand, within psuGat 2855.192 kb on - strand, within psuGat 2855.216 kb on + strand, within psuGat 2855.457 kb on - strandat 2855.464 kb on + strandat 2855.464 kb on + strandat 2855.503 kb on + strandat 2855.525 kb on + strandat 2855.598 kb on + strand, within psuTat 2855.599 kb on - strand, within psuTat 2855.623 kb on + strand, within psuTat 2855.623 kb on + strand, within psuTat 2855.624 kb on - strand, within psuTat 2855.624 kb on - strand, within psuTat 2855.624 kb on - strand, within psuTat 2855.638 kb on + strand, within psuTat 2855.661 kb on + strand, within psuTat 2855.758 kb on + strand, within psuTat 2855.803 kb on - strand, within psuTat 2855.805 kb on + strand, within psuTat 2855.939 kb on + strand, within psuTat 2855.979 kb on - strand, within psuTat 2856.031 kb on - strand, within psuTat 2856.046 kb on + strand, within psuTat 2856.057 kb on + strand, within psuTat 2856.058 kb on - strand, within psuTat 2856.111 kb on - strand, within psuTat 2856.117 kb on - strand, within psuTat 2856.273 kb on - strand, within psuTat 2856.293 kb on + strand, within psuTat 2856.560 kb on + strand, within psuTat 2857.108 kb on + strand, within yeiLat 2857.275 kb on + strand, within yeiLat 2857.275 kb on + strand, within yeiLat 2857.508 kb on + strandat 2857.513 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,854,804 + psuG MCAODC_14505 0.40 +0.3
2,855,166 - psuG MCAODC_14505 0.79 +0.5
2,855,166 - psuG MCAODC_14505 0.79 -1.0
2,855,192 - psuG MCAODC_14505 0.82 -1.9
2,855,216 + psuG MCAODC_14505 0.84 -0.2
2,855,457 - +0.5
2,855,464 + -0.2
2,855,464 + +0.6
2,855,503 + +1.5
2,855,525 + +1.6
2,855,598 + psuT MCAODC_14510 0.11 +1.2
2,855,599 - psuT MCAODC_14510 0.11 -0.6
2,855,623 + psuT MCAODC_14510 0.13 -1.0
2,855,623 + psuT MCAODC_14510 0.13 +0.5
2,855,624 - psuT MCAODC_14510 0.13 -1.0
2,855,624 - psuT MCAODC_14510 0.13 -1.5
2,855,624 - psuT MCAODC_14510 0.13 +0.7
2,855,638 + psuT MCAODC_14510 0.14 +0.4
2,855,661 + psuT MCAODC_14510 0.16 -0.7
2,855,758 + psuT MCAODC_14510 0.24 -0.8
2,855,803 - psuT MCAODC_14510 0.28 -0.4
2,855,805 + psuT MCAODC_14510 0.28 +0.1
2,855,939 + psuT MCAODC_14510 0.39 -0.5
2,855,979 - psuT MCAODC_14510 0.42 +0.5
2,856,031 - psuT MCAODC_14510 0.46 +0.3
2,856,046 + psuT MCAODC_14510 0.47 -1.4
2,856,057 + psuT MCAODC_14510 0.48 +1.0
2,856,058 - psuT MCAODC_14510 0.48 -0.4
2,856,111 - psuT MCAODC_14510 0.52 -1.2
2,856,117 - psuT MCAODC_14510 0.53 +1.8
2,856,273 - psuT MCAODC_14510 0.65 +1.0
2,856,293 + psuT MCAODC_14510 0.67 +0.1
2,856,560 + psuT MCAODC_14510 0.88 -1.8
2,857,108 + yeiL MCAODC_14515 0.47 +1.1
2,857,275 + yeiL MCAODC_14515 0.71 -1.0
2,857,275 + yeiL MCAODC_14515 0.71 +0.1
2,857,508 + -0.1
2,857,513 + +0.4

Or see this region's nucleotide sequence