Strain Fitness in Escherichia coli ECRC101 around MCAODC_11085

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypD and hypC overlap by 1 nucleotideshypC and hypB overlap by 10 nucleotideshypB and hypA are separated by 3 nucleotideshypA and hycA are separated by 211 nucleotides MCAODC_11075: hypD - hydrogenase formation protein HypD, at 2,207,808 to 2,208,929 hypD MCAODC_11080: hypC - hydrogenase 3 maturation protein HypC, at 2,208,929 to 2,209,201 hypC MCAODC_11085: hypB - hydrogenase nickel incorporation protein HypB, at 2,209,192 to 2,210,064 hypB MCAODC_11090: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,210,068 to 2,210,418 hypA MCAODC_11095: hycA - formate hydrogenlyase regulator HycA, at 2,210,630 to 2,211,091 hycA Position (kb) 2209 2210 2211Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2208.234 kb on + strand, within hypDat 2208.290 kb on + strand, within hypDat 2208.296 kb on + strand, within hypDat 2208.298 kb on + strand, within hypDat 2208.320 kb on + strand, within hypDat 2208.321 kb on - strand, within hypDat 2208.321 kb on - strand, within hypDat 2208.324 kb on - strand, within hypDat 2208.324 kb on - strand, within hypDat 2208.401 kb on + strand, within hypDat 2208.401 kb on + strand, within hypDat 2208.401 kb on + strand, within hypDat 2208.401 kb on + strand, within hypDat 2208.402 kb on - strand, within hypDat 2208.402 kb on - strand, within hypDat 2208.402 kb on - strand, within hypDat 2208.402 kb on - strand, within hypDat 2208.404 kb on + strand, within hypDat 2208.404 kb on + strand, within hypDat 2208.405 kb on - strand, within hypDat 2208.405 kb on - strand, within hypDat 2208.405 kb on - strand, within hypDat 2208.562 kb on + strand, within hypDat 2208.563 kb on - strand, within hypDat 2208.763 kb on - strand, within hypDat 2208.844 kb on + strandat 2208.844 kb on + strandat 2208.844 kb on + strandat 2208.845 kb on - strandat 2208.882 kb on + strandat 2208.883 kb on - strandat 2208.931 kb on - strandat 2208.945 kb on + strandat 2208.945 kb on + strandat 2209.024 kb on + strand, within hypCat 2209.024 kb on + strand, within hypCat 2209.025 kb on - strand, within hypCat 2209.025 kb on - strand, within hypCat 2209.025 kb on - strand, within hypCat 2209.029 kb on - strand, within hypCat 2209.029 kb on - strand, within hypCat 2209.052 kb on + strand, within hypCat 2209.058 kb on + strand, within hypCat 2209.194 kb on - strandat 2209.260 kb on + strandat 2209.384 kb on - strand, within hypBat 2209.474 kb on + strand, within hypBat 2209.691 kb on - strand, within hypBat 2209.887 kb on - strand, within hypBat 2209.936 kb on - strand, within hypBat 2210.008 kb on - strandat 2210.028 kb on - strandat 2210.028 kb on - strandat 2210.058 kb on + strandat 2210.059 kb on - strandat 2210.080 kb on + strandat 2210.081 kb on - strandat 2210.125 kb on + strand, within hypAat 2210.126 kb on - strand, within hypAat 2210.126 kb on - strand, within hypAat 2210.126 kb on - strand, within hypAat 2210.126 kb on - strand, within hypAat 2210.183 kb on + strand, within hypAat 2210.183 kb on + strand, within hypAat 2210.184 kb on - strand, within hypAat 2210.184 kb on - strand, within hypAat 2210.184 kb on - strand, within hypAat 2210.184 kb on - strand, within hypAat 2210.184 kb on - strand, within hypAat 2210.238 kb on + strand, within hypAat 2210.407 kb on + strandat 2210.407 kb on + strandat 2210.419 kb on - strandat 2210.431 kb on + strandat 2210.435 kb on + strandat 2210.436 kb on - strandat 2210.436 kb on - strandat 2210.488 kb on + strandat 2210.489 kb on - strandat 2210.561 kb on - strandat 2210.635 kb on + strandat 2210.635 kb on + strandat 2210.635 kb on + strandat 2210.636 kb on - strandat 2210.646 kb on + strandat 2210.646 kb on + strandat 2210.647 kb on - strandat 2210.708 kb on + strand, within hycAat 2210.709 kb on - strand, within hycAat 2210.709 kb on - strand, within hycAat 2210.822 kb on + strand, within hycAat 2210.822 kb on + strand, within hycAat 2210.822 kb on + strand, within hycAat 2210.823 kb on - strand, within hycAat 2210.823 kb on - strand, within hycAat 2210.823 kb on - strand, within hycAat 2210.864 kb on + strand, within hycAat 2210.864 kb on + strand, within hycAat 2210.864 kb on + strand, within hycAat 2210.865 kb on - strand, within hycAat 2210.867 kb on + strand, within hycAat 2210.867 kb on + strand, within hycAat 2210.867 kb on + strand, within hycAat 2210.984 kb on + strand, within hycAat 2210.984 kb on + strand, within hycAat 2210.984 kb on + strand, within hycAat 2210.985 kb on - strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.018 kb on + strand, within hycAat 2211.019 kb on - strand, within hycAat 2211.019 kb on - strand, within hycAat 2211.019 kb on - strand, within hycAat 2211.019 kb on - strand, within hycAat 2211.019 kb on - strand, within hycAat 2211.020 kb on + strand, within hycAat 2211.020 kb on + strand, within hycAat 2211.020 kb on + strand, within hycAat 2211.021 kb on - strand, within hycAat 2211.054 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,208,234 + hypD MCAODC_11075 0.38 -1.5
2,208,290 + hypD MCAODC_11075 0.43 -0.5
2,208,296 + hypD MCAODC_11075 0.43 +0.2
2,208,298 + hypD MCAODC_11075 0.44 -2.4
2,208,320 + hypD MCAODC_11075 0.46 -0.6
2,208,321 - hypD MCAODC_11075 0.46 +0.2
2,208,321 - hypD MCAODC_11075 0.46 -0.7
2,208,324 - hypD MCAODC_11075 0.46 +0.3
2,208,324 - hypD MCAODC_11075 0.46 +0.4
2,208,401 + hypD MCAODC_11075 0.53 +0.8
2,208,401 + hypD MCAODC_11075 0.53 -0.7
2,208,401 + hypD MCAODC_11075 0.53 +0.3
2,208,401 + hypD MCAODC_11075 0.53 +1.1
2,208,402 - hypD MCAODC_11075 0.53 +0.6
2,208,402 - hypD MCAODC_11075 0.53 +0.8
2,208,402 - hypD MCAODC_11075 0.53 -0.1
2,208,402 - hypD MCAODC_11075 0.53 +0.6
2,208,404 + hypD MCAODC_11075 0.53 -0.5
2,208,404 + hypD MCAODC_11075 0.53 -0.4
2,208,405 - hypD MCAODC_11075 0.53 +1.0
2,208,405 - hypD MCAODC_11075 0.53 +0.5
2,208,405 - hypD MCAODC_11075 0.53 +1.2
2,208,562 + hypD MCAODC_11075 0.67 -1.8
2,208,563 - hypD MCAODC_11075 0.67 -0.0
2,208,763 - hypD MCAODC_11075 0.85 +0.2
2,208,844 + +0.3
2,208,844 + +0.6
2,208,844 + -0.5
2,208,845 - +0.7
2,208,882 + -1.4
2,208,883 - -1.6
2,208,931 - -1.0
2,208,945 + -2.6
2,208,945 + -1.6
2,209,024 + hypC MCAODC_11080 0.35 -2.8
2,209,024 + hypC MCAODC_11080 0.35 +1.2
2,209,025 - hypC MCAODC_11080 0.35 +0.2
2,209,025 - hypC MCAODC_11080 0.35 -1.0
2,209,025 - hypC MCAODC_11080 0.35 +0.3
2,209,029 - hypC MCAODC_11080 0.37 +0.1
2,209,029 - hypC MCAODC_11080 0.37 -0.0
2,209,052 + hypC MCAODC_11080 0.45 +0.9
2,209,058 + hypC MCAODC_11080 0.47 +0.1
2,209,194 - +1.0
2,209,260 + -1.0
2,209,384 - hypB MCAODC_11085 0.22 -0.0
2,209,474 + hypB MCAODC_11085 0.32 +0.5
2,209,691 - hypB MCAODC_11085 0.57 -0.3
2,209,887 - hypB MCAODC_11085 0.80 +1.0
2,209,936 - hypB MCAODC_11085 0.85 +1.5
2,210,008 - +0.7
2,210,028 - +1.4
2,210,028 - +0.7
2,210,058 + +0.7
2,210,059 - -0.9
2,210,080 + -0.4
2,210,081 - +0.0
2,210,125 + hypA MCAODC_11090 0.16 -0.2
2,210,126 - hypA MCAODC_11090 0.17 -0.2
2,210,126 - hypA MCAODC_11090 0.17 -0.2
2,210,126 - hypA MCAODC_11090 0.17 -1.8
2,210,126 - hypA MCAODC_11090 0.17 +0.7
2,210,183 + hypA MCAODC_11090 0.33 +1.4
2,210,183 + hypA MCAODC_11090 0.33 +0.6
2,210,184 - hypA MCAODC_11090 0.33 +0.3
2,210,184 - hypA MCAODC_11090 0.33 -0.1
2,210,184 - hypA MCAODC_11090 0.33 +0.3
2,210,184 - hypA MCAODC_11090 0.33 -0.2
2,210,184 - hypA MCAODC_11090 0.33 +0.5
2,210,238 + hypA MCAODC_11090 0.48 +0.1
2,210,407 + +0.8
2,210,407 + +0.2
2,210,419 - -1.1
2,210,431 + +0.9
2,210,435 + +0.6
2,210,436 - +0.5
2,210,436 - +1.0
2,210,488 + +1.0
2,210,489 - +1.8
2,210,561 - +0.1
2,210,635 + -0.2
2,210,635 + -0.4
2,210,635 + +0.2
2,210,636 - +0.9
2,210,646 + +0.2
2,210,646 + +0.1
2,210,647 - -1.1
2,210,708 + hycA MCAODC_11095 0.17 +0.4
2,210,709 - hycA MCAODC_11095 0.17 +0.2
2,210,709 - hycA MCAODC_11095 0.17 +1.6
2,210,822 + hycA MCAODC_11095 0.42 -0.4
2,210,822 + hycA MCAODC_11095 0.42 +0.2
2,210,822 + hycA MCAODC_11095 0.42 +1.3
2,210,823 - hycA MCAODC_11095 0.42 +0.4
2,210,823 - hycA MCAODC_11095 0.42 +0.6
2,210,823 - hycA MCAODC_11095 0.42 +0.2
2,210,864 + hycA MCAODC_11095 0.51 +0.4
2,210,864 + hycA MCAODC_11095 0.51 -0.7
2,210,864 + hycA MCAODC_11095 0.51 +0.1
2,210,865 - hycA MCAODC_11095 0.51 -0.0
2,210,867 + hycA MCAODC_11095 0.51 -3.7
2,210,867 + hycA MCAODC_11095 0.51 +0.6
2,210,867 + hycA MCAODC_11095 0.51 +0.7
2,210,984 + hycA MCAODC_11095 0.77 +0.5
2,210,984 + hycA MCAODC_11095 0.77 +1.2
2,210,984 + hycA MCAODC_11095 0.77 +0.3
2,210,985 - hycA MCAODC_11095 0.77 +0.8
2,211,018 + hycA MCAODC_11095 0.84 +0.2
2,211,018 + hycA MCAODC_11095 0.84 +0.2
2,211,018 + hycA MCAODC_11095 0.84 -0.0
2,211,018 + hycA MCAODC_11095 0.84 -2.7
2,211,018 + hycA MCAODC_11095 0.84 -0.3
2,211,018 + hycA MCAODC_11095 0.84 +1.5
2,211,019 - hycA MCAODC_11095 0.84 -1.1
2,211,019 - hycA MCAODC_11095 0.84 +1.9
2,211,019 - hycA MCAODC_11095 0.84 -1.6
2,211,019 - hycA MCAODC_11095 0.84 +1.0
2,211,019 - hycA MCAODC_11095 0.84 +0.7
2,211,020 + hycA MCAODC_11095 0.84 +2.4
2,211,020 + hycA MCAODC_11095 0.84 -0.6
2,211,020 + hycA MCAODC_11095 0.84 -0.6
2,211,021 - hycA MCAODC_11095 0.85 +0.1
2,211,054 + +1.7

Or see this region's nucleotide sequence