Strain Fitness in Escherichia coli ECRC101 around MCAODC_03225

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrbsA and MCAODC_03225 overlap by 4 nucleotidesMCAODC_03225 and rbsB are separated by 18 nucleotides MCAODC_03220: rbsA - Ribose import ATP-binding protein RbsA, at 647,726 to 649,246 rbsA MCAODC_03225: MCAODC_03225 - ribose ABC transporter permease, at 649,243 to 650,217 _03225 MCAODC_03230: rbsB - transcriptional regulator, at 650,236 to 651,177 rbsB Position (kb) 649 650 651Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 648.280 kb on + strand, within rbsAat 648.281 kb on - strand, within rbsAat 648.311 kb on - strand, within rbsAat 648.311 kb on - strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.314 kb on + strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.315 kb on - strand, within rbsAat 648.341 kb on + strand, within rbsAat 648.341 kb on + strand, within rbsAat 648.342 kb on - strand, within rbsAat 648.342 kb on - strand, within rbsAat 648.370 kb on - strand, within rbsAat 648.383 kb on + strand, within rbsAat 648.384 kb on - strand, within rbsAat 648.384 kb on - strand, within rbsAat 648.469 kb on + strand, within rbsAat 648.756 kb on + strand, within rbsAat 648.757 kb on - strand, within rbsAat 648.868 kb on + strand, within rbsAat 648.868 kb on + strand, within rbsAat 648.869 kb on - strand, within rbsAat 648.869 kb on - strand, within rbsAat 648.869 kb on - strand, within rbsAat 648.904 kb on - strand, within rbsAat 648.904 kb on - strand, within rbsAat 648.904 kb on - strand, within rbsAat 649.104 kb on + strandat 649.127 kb on + strandat 649.127 kb on + strandat 649.128 kb on - strandat 649.128 kb on - strandat 649.161 kb on + strandat 649.162 kb on - strandat 649.192 kb on + strandat 649.192 kb on + strandat 649.192 kb on + strandat 649.193 kb on - strandat 649.193 kb on - strandat 649.193 kb on - strandat 649.193 kb on - strandat 649.200 kb on + strandat 649.201 kb on - strandat 649.389 kb on + strand, within MCAODC_03225at 649.522 kb on - strand, within MCAODC_03225at 649.645 kb on + strand, within MCAODC_03225at 649.645 kb on + strand, within MCAODC_03225at 649.646 kb on - strand, within MCAODC_03225at 649.646 kb on - strand, within MCAODC_03225at 649.646 kb on - strand, within MCAODC_03225at 649.648 kb on - strand, within MCAODC_03225at 649.648 kb on - strand, within MCAODC_03225at 649.695 kb on + strand, within MCAODC_03225at 649.710 kb on + strand, within MCAODC_03225at 649.735 kb on + strand, within MCAODC_03225at 649.735 kb on + strand, within MCAODC_03225at 649.735 kb on + strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.736 kb on - strand, within MCAODC_03225at 649.827 kb on + strand, within MCAODC_03225at 649.827 kb on + strand, within MCAODC_03225at 649.828 kb on - strand, within MCAODC_03225at 649.828 kb on - strand, within MCAODC_03225at 649.828 kb on - strand, within MCAODC_03225at 649.831 kb on + strand, within MCAODC_03225at 649.831 kb on + strand, within MCAODC_03225at 649.867 kb on - strand, within MCAODC_03225at 649.867 kb on - strand, within MCAODC_03225at 649.875 kb on + strand, within MCAODC_03225at 649.875 kb on + strand, within MCAODC_03225at 649.876 kb on - strand, within MCAODC_03225at 649.904 kb on - strand, within MCAODC_03225at 649.960 kb on + strand, within MCAODC_03225at 649.979 kb on + strand, within MCAODC_03225at 649.979 kb on + strand, within MCAODC_03225at 649.980 kb on - strand, within MCAODC_03225at 649.980 kb on - strand, within MCAODC_03225at 649.980 kb on - strand, within MCAODC_03225at 649.980 kb on - strand, within MCAODC_03225at 650.081 kb on + strand, within MCAODC_03225at 650.085 kb on + strand, within MCAODC_03225at 650.128 kb on + strandat 650.128 kb on + strandat 650.128 kb on + strandat 650.129 kb on - strandat 650.129 kb on - strandat 650.189 kb on - strandat 650.230 kb on + strandat 650.231 kb on - strandat 650.231 kb on - strandat 650.331 kb on - strand, within rbsBat 650.444 kb on - strand, within rbsBat 650.471 kb on + strand, within rbsBat 650.472 kb on - strand, within rbsBat 650.472 kb on - strand, within rbsBat 650.505 kb on + strand, within rbsBat 650.592 kb on - strand, within rbsBat 650.655 kb on + strand, within rbsBat 650.655 kb on + strand, within rbsBat 650.656 kb on - strand, within rbsBat 650.656 kb on - strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.659 kb on + strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.660 kb on - strand, within rbsBat 650.756 kb on - strand, within rbsBat 650.834 kb on + strand, within rbsBat 650.834 kb on + strand, within rbsBat 650.834 kb on + strand, within rbsBat 650.835 kb on - strand, within rbsBat 650.876 kb on + strand, within rbsBat 650.876 kb on + strand, within rbsBat 650.938 kb on - strand, within rbsBat 650.949 kb on + strand, within rbsBat 651.154 kb on + strandat 651.155 kb on - strandat 651.155 kb on - strandat 651.175 kb on + strandat 651.175 kb on + strandat 651.176 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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648,280 + rbsA MCAODC_03220 0.36 -2.3
648,281 - rbsA MCAODC_03220 0.36 +0.4
648,311 - rbsA MCAODC_03220 0.38 +0.4
648,311 - rbsA MCAODC_03220 0.38 -0.5
648,314 + rbsA MCAODC_03220 0.39 -0.5
648,314 + rbsA MCAODC_03220 0.39 +0.9
648,314 + rbsA MCAODC_03220 0.39 +1.1
648,314 + rbsA MCAODC_03220 0.39 -0.5
648,314 + rbsA MCAODC_03220 0.39 -0.7
648,314 + rbsA MCAODC_03220 0.39 +1.1
648,315 - rbsA MCAODC_03220 0.39 -0.6
648,315 - rbsA MCAODC_03220 0.39 -0.3
648,315 - rbsA MCAODC_03220 0.39 -4.1
648,315 - rbsA MCAODC_03220 0.39 +0.0
648,315 - rbsA MCAODC_03220 0.39 -0.8
648,315 - rbsA MCAODC_03220 0.39 -1.0
648,341 + rbsA MCAODC_03220 0.40 +1.3
648,341 + rbsA MCAODC_03220 0.40 +1.3
648,342 - rbsA MCAODC_03220 0.40 +0.1
648,342 - rbsA MCAODC_03220 0.40 +0.0
648,370 - rbsA MCAODC_03220 0.42 +1.0
648,383 + rbsA MCAODC_03220 0.43 -0.8
648,384 - rbsA MCAODC_03220 0.43 +0.2
648,384 - rbsA MCAODC_03220 0.43 -0.1
648,469 + rbsA MCAODC_03220 0.49 +0.8
648,756 + rbsA MCAODC_03220 0.68 -0.7
648,757 - rbsA MCAODC_03220 0.68 -0.0
648,868 + rbsA MCAODC_03220 0.75 -0.5
648,868 + rbsA MCAODC_03220 0.75 -0.4
648,869 - rbsA MCAODC_03220 0.75 +0.8
648,869 - rbsA MCAODC_03220 0.75 +1.6
648,869 - rbsA MCAODC_03220 0.75 +0.8
648,904 - rbsA MCAODC_03220 0.77 -1.8
648,904 - rbsA MCAODC_03220 0.77 -0.3
648,904 - rbsA MCAODC_03220 0.77 +0.8
649,104 + +0.4
649,127 + +0.9
649,127 + +0.9
649,128 - -0.5
649,128 - +0.8
649,161 + +0.5
649,162 - +1.5
649,192 + -0.4
649,192 + -2.6
649,192 + -0.7
649,193 - +1.6
649,193 - -0.2
649,193 - +0.2
649,193 - +0.6
649,200 + -1.3
649,201 - +0.0
649,389 + MCAODC_03225 0.15 +0.2
649,522 - MCAODC_03225 0.29 -2.5
649,645 + MCAODC_03225 0.41 +0.8
649,645 + MCAODC_03225 0.41 -0.2
649,646 - MCAODC_03225 0.41 -1.6
649,646 - MCAODC_03225 0.41 -1.4
649,646 - MCAODC_03225 0.41 +1.2
649,648 - MCAODC_03225 0.42 -0.2
649,648 - MCAODC_03225 0.42 -0.2
649,695 + MCAODC_03225 0.46 -0.6
649,710 + MCAODC_03225 0.48 -0.1
649,735 + MCAODC_03225 0.50 -4.5
649,735 + MCAODC_03225 0.50 +2.9
649,735 + MCAODC_03225 0.50 +0.2
649,736 - MCAODC_03225 0.51 +0.2
649,736 - MCAODC_03225 0.51 +1.2
649,736 - MCAODC_03225 0.51 -0.0
649,736 - MCAODC_03225 0.51 +0.1
649,736 - MCAODC_03225 0.51 -0.4
649,736 - MCAODC_03225 0.51 +0.7
649,736 - MCAODC_03225 0.51 -0.5
649,827 + MCAODC_03225 0.60 +1.1
649,827 + MCAODC_03225 0.60 -0.1
649,828 - MCAODC_03225 0.60 -0.6
649,828 - MCAODC_03225 0.60 +1.0
649,828 - MCAODC_03225 0.60 -1.5
649,831 + MCAODC_03225 0.60 +0.1
649,831 + MCAODC_03225 0.60 +0.6
649,867 - MCAODC_03225 0.64 +0.3
649,867 - MCAODC_03225 0.64 +0.9
649,875 + MCAODC_03225 0.65 -0.4
649,875 + MCAODC_03225 0.65 -0.1
649,876 - MCAODC_03225 0.65 -0.5
649,904 - MCAODC_03225 0.68 -1.5
649,960 + MCAODC_03225 0.74 +0.8
649,979 + MCAODC_03225 0.75 +0.6
649,979 + MCAODC_03225 0.75 -4.1
649,980 - MCAODC_03225 0.76 -0.1
649,980 - MCAODC_03225 0.76 +0.2
649,980 - MCAODC_03225 0.76 +0.0
649,980 - MCAODC_03225 0.76 +0.4
650,081 + MCAODC_03225 0.86 -0.9
650,085 + MCAODC_03225 0.86 +0.6
650,128 + -1.5
650,128 + +0.9
650,128 + +0.1
650,129 - -0.2
650,129 - +0.2
650,189 - +1.2
650,230 + +1.8
650,231 - +0.6
650,231 - +0.1
650,331 - rbsB MCAODC_03230 0.10 +0.7
650,444 - rbsB MCAODC_03230 0.22 -0.3
650,471 + rbsB MCAODC_03230 0.25 +0.9
650,472 - rbsB MCAODC_03230 0.25 -2.8
650,472 - rbsB MCAODC_03230 0.25 -0.4
650,505 + rbsB MCAODC_03230 0.29 +1.1
650,592 - rbsB MCAODC_03230 0.38 -1.2
650,655 + rbsB MCAODC_03230 0.44 +0.5
650,655 + rbsB MCAODC_03230 0.44 -0.1
650,656 - rbsB MCAODC_03230 0.45 +1.2
650,656 - rbsB MCAODC_03230 0.45 +0.8
650,659 + rbsB MCAODC_03230 0.45 -0.0
650,659 + rbsB MCAODC_03230 0.45 +0.1
650,659 + rbsB MCAODC_03230 0.45 +1.0
650,659 + rbsB MCAODC_03230 0.45 +1.6
650,659 + rbsB MCAODC_03230 0.45 +0.9
650,659 + rbsB MCAODC_03230 0.45 -0.1
650,659 + rbsB MCAODC_03230 0.45 -1.0
650,660 - rbsB MCAODC_03230 0.45 +0.8
650,660 - rbsB MCAODC_03230 0.45 -0.1
650,660 - rbsB MCAODC_03230 0.45 -0.0
650,660 - rbsB MCAODC_03230 0.45 +0.5
650,660 - rbsB MCAODC_03230 0.45 -1.0
650,660 - rbsB MCAODC_03230 0.45 -0.5
650,660 - rbsB MCAODC_03230 0.45 +0.2
650,756 - rbsB MCAODC_03230 0.55 -0.6
650,834 + rbsB MCAODC_03230 0.63 +0.1
650,834 + rbsB MCAODC_03230 0.63 +1.4
650,834 + rbsB MCAODC_03230 0.63 -0.6
650,835 - rbsB MCAODC_03230 0.64 -1.4
650,876 + rbsB MCAODC_03230 0.68 +0.6
650,876 + rbsB MCAODC_03230 0.68 -0.2
650,938 - rbsB MCAODC_03230 0.75 -0.8
650,949 + rbsB MCAODC_03230 0.76 -1.7
651,154 + +0.4
651,155 - +0.8
651,155 - -0.5
651,175 + -1.0
651,175 + +0.6
651,176 - +1.0

Or see this region's nucleotide sequence