Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC1 and leuD are separated by 10 nucleotides leuD and sgrR are separated by 327 nucleotides
MCAODC_01240: leuC1 - 3-isopropylmalate dehydratase large subunit 1, at 231,739 to 233,139
leuC1
MCAODC_01245: leuD - 3-isopropylmalate dehydratase small subunit, at 233,150 to 233,755
leuD
MCAODC_01255: sgrR - DNA-binding transcriptional regulator SgrR, at 234,083 to 235,741
sgrR
Position (kb) 233
234 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 232.193 kb on + strand, within leuC1 at 232.193 kb on + strand, within leuC1 at 232.194 kb on - strand, within leuC1 at 232.285 kb on - strand, within leuC1 at 232.299 kb on + strand, within leuC1 at 232.370 kb on + strand, within leuC1 at 232.371 kb on - strand, within leuC1 at 232.402 kb on - strand, within leuC1 at 232.402 kb on - strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.458 kb on + strand, within leuC1 at 232.459 kb on - strand, within leuC1 at 232.459 kb on - strand, within leuC1 at 232.462 kb on + strand, within leuC1 at 232.614 kb on + strand, within leuC1 at 232.647 kb on + strand, within leuC1 at 232.711 kb on + strand, within leuC1 at 232.711 kb on + strand, within leuC1 at 232.711 kb on + strand, within leuC1 at 232.711 kb on + strand, within leuC1 at 232.711 kb on + strand, within leuC1 at 232.712 kb on - strand, within leuC1 at 232.712 kb on - strand, within leuC1 at 232.713 kb on + strand, within leuC1 at 232.713 kb on + strand, within leuC1 at 232.714 kb on - strand, within leuC1 at 232.792 kb on - strand, within leuC1 at 232.792 kb on - strand, within leuC1 at 232.792 kb on - strand, within leuC1 at 232.851 kb on - strand, within leuC1 at 232.915 kb on - strand, within leuC1 at 233.029 kb on - strand at 233.133 kb on + strand at 233.133 kb on + strand at 233.134 kb on - strand at 233.137 kb on + strand at 233.137 kb on + strand at 233.138 kb on - strand at 233.351 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.481 kb on - strand, within leuD at 233.488 kb on + strand, within leuD at 233.488 kb on + strand, within leuD at 233.489 kb on - strand, within leuD at 233.489 kb on - strand, within leuD at 233.521 kb on - strand, within leuD at 233.586 kb on + strand, within leuD at 233.587 kb on - strand, within leuD at 233.628 kb on - strand, within leuD at 233.692 kb on + strand, within leuD at 233.693 kb on - strand, within leuD at 233.747 kb on - strand at 233.747 kb on - strand at 233.753 kb on + strand at 233.754 kb on - strand at 233.760 kb on + strand at 233.805 kb on + strand at 233.805 kb on + strand at 233.806 kb on - strand at 233.828 kb on - strand at 233.836 kb on + strand at 233.836 kb on + strand at 233.837 kb on - strand at 233.914 kb on + strand at 233.972 kb on + strand at 233.972 kb on + strand at 233.973 kb on - strand at 233.973 kb on - strand at 234.019 kb on - strand at 234.019 kb on - strand at 234.025 kb on + strand at 234.033 kb on + strand at 234.033 kb on + strand at 234.034 kb on - strand at 234.036 kb on + strand at 234.036 kb on + strand at 234.039 kb on - strand at 234.039 kb on - strand at 234.139 kb on + strand at 234.139 kb on + strand at 234.140 kb on - strand at 234.177 kb on + strand at 234.200 kb on - strand at 234.200 kb on - strand at 234.268 kb on + strand, within sgrR at 234.269 kb on - strand, within sgrR at 234.293 kb on + strand, within sgrR at 234.293 kb on + strand, within sgrR at 234.294 kb on - strand, within sgrR at 234.294 kb on - strand, within sgrR at 234.361 kb on - strand, within sgrR at 234.459 kb on - strand, within sgrR at 234.461 kb on + strand, within sgrR at 234.461 kb on + strand, within sgrR at 234.475 kb on + strand, within sgrR at 234.476 kb on - strand, within sgrR at 234.552 kb on + strand, within sgrR at 234.552 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.710 kb on - strand, within sgrR at 234.722 kb on + strand, within sgrR at 234.730 kb on + strand, within sgrR
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 232,193 + leuC1 MCAODC_01240 0.32 -5.3 232,193 + leuC1 MCAODC_01240 0.32 -2.1 232,194 - leuC1 MCAODC_01240 0.32 -0.2 232,285 - leuC1 MCAODC_01240 0.39 -4.3 232,299 + leuC1 MCAODC_01240 0.40 -1.6 232,370 + leuC1 MCAODC_01240 0.45 -4.2 232,371 - leuC1 MCAODC_01240 0.45 -3.7 232,402 - leuC1 MCAODC_01240 0.47 -4.2 232,402 - leuC1 MCAODC_01240 0.47 -3.5 232,458 + leuC1 MCAODC_01240 0.51 -2.2 232,458 + leuC1 MCAODC_01240 0.51 -5.0 232,458 + leuC1 MCAODC_01240 0.51 -5.2 232,458 + leuC1 MCAODC_01240 0.51 -3.1 232,458 + leuC1 MCAODC_01240 0.51 -5.0 232,458 + leuC1 MCAODC_01240 0.51 -2.1 232,458 + leuC1 MCAODC_01240 0.51 -3.6 232,458 + leuC1 MCAODC_01240 0.51 -4.6 232,458 + leuC1 MCAODC_01240 0.51 -3.5 232,458 + leuC1 MCAODC_01240 0.51 -1.6 232,458 + leuC1 MCAODC_01240 0.51 -4.5 232,459 - leuC1 MCAODC_01240 0.51 -4.1 232,459 - leuC1 MCAODC_01240 0.51 -4.2 232,462 + leuC1 MCAODC_01240 0.52 -3.3 232,614 + leuC1 MCAODC_01240 0.62 -3.9 232,647 + leuC1 MCAODC_01240 0.65 -5.2 232,711 + leuC1 MCAODC_01240 0.69 -3.1 232,711 + leuC1 MCAODC_01240 0.69 -4.3 232,711 + leuC1 MCAODC_01240 0.69 -2.7 232,711 + leuC1 MCAODC_01240 0.69 -1.2 232,711 + leuC1 MCAODC_01240 0.69 -1.6 232,712 - leuC1 MCAODC_01240 0.69 -3.0 232,712 - leuC1 MCAODC_01240 0.69 -6.1 232,713 + leuC1 MCAODC_01240 0.70 -3.4 232,713 + leuC1 MCAODC_01240 0.70 -2.6 232,714 - leuC1 MCAODC_01240 0.70 -3.3 232,792 - leuC1 MCAODC_01240 0.75 -2.3 232,792 - leuC1 MCAODC_01240 0.75 -3.7 232,792 - leuC1 MCAODC_01240 0.75 -5.1 232,851 - leuC1 MCAODC_01240 0.79 -1.7 232,915 - leuC1 MCAODC_01240 0.84 -3.8 233,029 - -3.8 233,133 + -0.0 233,133 + +0.4 233,134 - -4.1 233,137 + -1.1 233,137 + -0.4 233,138 - -4.1 233,351 + leuD MCAODC_01245 0.33 -4.0 233,443 + leuD MCAODC_01245 0.48 -3.2 233,443 + leuD MCAODC_01245 0.48 -4.4 233,443 + leuD MCAODC_01245 0.48 +0.3 233,481 - leuD MCAODC_01245 0.55 -2.2 233,488 + leuD MCAODC_01245 0.56 -4.0 233,488 + leuD MCAODC_01245 0.56 -2.4 233,489 - leuD MCAODC_01245 0.56 -3.5 233,489 - leuD MCAODC_01245 0.56 -5.5 233,521 - leuD MCAODC_01245 0.61 -4.3 233,586 + leuD MCAODC_01245 0.72 -4.9 233,587 - leuD MCAODC_01245 0.72 -1.8 233,628 - leuD MCAODC_01245 0.79 -3.6 233,692 + leuD MCAODC_01245 0.89 -4.0 233,693 - leuD MCAODC_01245 0.90 -3.4 233,747 - +1.5 233,747 - +0.4 233,753 + -0.6 233,754 - +0.6 233,760 + -0.5 233,805 + -1.0 233,805 + +0.1 233,806 - +0.1 233,828 - +0.8 233,836 + +0.5 233,836 + -0.1 233,837 - +1.3 233,914 + +0.0 233,972 + -1.8 233,972 + +0.2 233,973 - -0.4 233,973 - -0.8 234,019 - +0.6 234,019 - +0.5 234,025 + +0.2 234,033 + +1.1 234,033 + +0.8 234,034 - -0.5 234,036 + -0.5 234,036 + +2.9 234,039 - -0.1 234,039 - -0.1 234,139 + +0.5 234,139 + +0.4 234,140 - +0.0 234,177 + +1.7 234,200 - +0.3 234,200 - +0.9 234,268 + sgrR MCAODC_01255 0.11 -0.5 234,269 - sgrR MCAODC_01255 0.11 -0.1 234,293 + sgrR MCAODC_01255 0.13 +0.1 234,293 + sgrR MCAODC_01255 0.13 -0.2 234,294 - sgrR MCAODC_01255 0.13 +0.5 234,294 - sgrR MCAODC_01255 0.13 -0.7 234,361 - sgrR MCAODC_01255 0.17 -1.1 234,459 - sgrR MCAODC_01255 0.23 +0.1 234,461 + sgrR MCAODC_01255 0.23 -0.2 234,461 + sgrR MCAODC_01255 0.23 +0.0 234,475 + sgrR MCAODC_01255 0.24 +2.2 234,476 - sgrR MCAODC_01255 0.24 -0.2 234,552 + sgrR MCAODC_01255 0.28 +0.5 234,552 + sgrR MCAODC_01255 0.28 -1.7 234,606 + sgrR MCAODC_01255 0.32 -0.8 234,606 + sgrR MCAODC_01255 0.32 -0.1 234,606 + sgrR MCAODC_01255 0.32 +0.8 234,710 - sgrR MCAODC_01255 0.38 +0.3 234,722 + sgrR MCAODC_01255 0.39 -0.1 234,730 + sgrR MCAODC_01255 0.39 +0.3
Or see this region's nucleotide sequence