Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and gpt are separated by 91 nucleotides gpt and pepD are separated by 260 nucleotides pepD and prfH are separated by 56 nucleotides prfH and ykfJ overlap by 32 nucleotides
MCAODC_00210: frsA - esterase FrsA, at 18,936 to 20,180
frsA
MCAODC_00215: gpt - xanthine phosphoribosyltransferase, at 20,272 to 20,730
gpt
MCAODC_00220: pepD - cytosol nonspecific dipeptidase, at 20,991 to 22,448
pepD
MCAODC_00225: prfH - peptide chain release factor H, at 22,505 to 23,005
prfH
MCAODC_00230: ykfJ - Putative uncharacterized protein YkfJ, at 22,974 to 23,240
ykfJ
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 20.058 kb on - strand at 20.058 kb on - strand at 20.058 kb on - strand at 20.099 kb on + strand at 20.136 kb on - strand at 20.198 kb on + strand at 20.198 kb on + strand at 20.198 kb on + strand at 20.202 kb on + strand at 20.202 kb on + strand at 20.212 kb on + strand at 20.217 kb on + strand at 20.217 kb on + strand at 20.217 kb on + strand at 20.217 kb on + strand at 20.357 kb on + strand, within gpt at 20.358 kb on - strand, within gpt at 20.473 kb on - strand, within gpt at 20.517 kb on + strand, within gpt at 20.518 kb on - strand, within gpt at 20.518 kb on - strand, within gpt at 20.543 kb on + strand, within gpt at 20.544 kb on - strand, within gpt at 20.553 kb on + strand, within gpt at 20.554 kb on - strand, within gpt at 20.635 kb on - strand, within gpt at 20.680 kb on - strand, within gpt at 20.717 kb on + strand at 20.717 kb on + strand at 20.718 kb on - strand at 20.755 kb on + strand at 20.763 kb on - strand at 20.785 kb on - strand at 20.800 kb on - strand at 20.802 kb on - strand at 20.827 kb on + strand at 20.899 kb on + strand at 20.899 kb on + strand at 20.900 kb on - strand at 20.900 kb on - strand at 20.987 kb on + strand at 20.987 kb on + strand at 20.988 kb on - strand at 20.988 kb on - strand at 20.988 kb on - strand at 21.010 kb on + strand at 21.053 kb on + strand at 21.054 kb on - strand at 21.054 kb on - strand at 21.054 kb on - strand at 21.054 kb on - strand at 21.054 kb on - strand at 21.069 kb on + strand at 21.093 kb on + strand at 21.093 kb on + strand at 21.093 kb on + strand at 21.093 kb on + strand at 21.093 kb on + strand at 21.149 kb on + strand, within pepD at 21.149 kb on + strand, within pepD at 21.149 kb on + strand, within pepD at 21.149 kb on + strand, within pepD at 21.150 kb on - strand, within pepD at 21.150 kb on - strand, within pepD at 21.152 kb on + strand, within pepD at 21.152 kb on + strand, within pepD at 21.153 kb on - strand, within pepD at 21.182 kb on + strand, within pepD at 21.183 kb on - strand, within pepD at 21.194 kb on + strand, within pepD at 21.242 kb on + strand, within pepD at 21.276 kb on - strand, within pepD at 21.285 kb on + strand, within pepD at 21.286 kb on - strand, within pepD at 21.286 kb on - strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.287 kb on + strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.288 kb on - strand, within pepD at 21.347 kb on + strand, within pepD at 21.488 kb on + strand, within pepD at 21.488 kb on + strand, within pepD at 21.519 kb on + strand, within pepD at 21.519 kb on + strand, within pepD at 21.519 kb on + strand, within pepD at 21.566 kb on - strand, within pepD at 21.613 kb on + strand, within pepD at 21.613 kb on + strand, within pepD at 21.614 kb on - strand, within pepD at 21.614 kb on - strand, within pepD at 21.614 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.834 kb on - strand, within pepD at 21.837 kb on + strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.838 kb on - strand, within pepD at 21.914 kb on + strand, within pepD at 21.914 kb on + strand, within pepD at 21.951 kb on - strand, within pepD at 22.063 kb on + strand, within pepD at 22.110 kb on + strand, within pepD at 22.111 kb on - strand, within pepD at 22.111 kb on - strand, within pepD at 22.111 kb on - strand, within pepD at 22.176 kb on + strand, within pepD at 22.222 kb on + strand, within pepD at 22.334 kb on + strand at 22.393 kb on + strand at 22.393 kb on + strand at 22.393 kb on + strand at 22.394 kb on - strand at 22.447 kb on - strand at 22.451 kb on - strand at 22.517 kb on - strand at 22.603 kb on + strand, within prfH at 22.604 kb on - strand, within prfH at 22.604 kb on - strand, within prfH at 22.664 kb on - strand, within prfH at 22.728 kb on - strand, within prfH at 22.825 kb on + strand, within prfH at 22.825 kb on + strand, within prfH at 22.981 kb on - strand at 23.085 kb on + strand, within ykfJ at 23.103 kb on + strand, within ykfJ at 23.140 kb on - strand, within ykfJ at 23.189 kb on + strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.194 kb on - strand, within ykfJ at 23.216 kb on + strand at 23.217 kb on - strand at 23.218 kb on + strand at 23.219 kb on - strand at 23.231 kb on - strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.243 kb on - strand at 23.254 kb on - strand at 23.254 kb on - strand at 23.306 kb on - strand at 23.310 kb on - strand at 23.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 20,058 - -1.5 20,058 - +0.0 20,058 - +0.9 20,099 + -1.6 20,136 - -1.2 20,198 + -0.3 20,198 + -0.8 20,198 + +0.4 20,202 + -0.6 20,202 + +0.7 20,212 + +0.3 20,217 + +0.5 20,217 + -0.3 20,217 + -0.0 20,217 + +0.4 20,357 + gpt MCAODC_00215 0.19 -0.2 20,358 - gpt MCAODC_00215 0.19 -1.0 20,473 - gpt MCAODC_00215 0.44 -2.0 20,517 + gpt MCAODC_00215 0.53 +0.2 20,518 - gpt MCAODC_00215 0.54 -1.0 20,518 - gpt MCAODC_00215 0.54 -1.1 20,543 + gpt MCAODC_00215 0.59 -1.0 20,544 - gpt MCAODC_00215 0.59 +0.6 20,553 + gpt MCAODC_00215 0.61 +0.4 20,554 - gpt MCAODC_00215 0.61 +0.0 20,635 - gpt MCAODC_00215 0.79 -1.1 20,680 - gpt MCAODC_00215 0.89 -0.5 20,717 + -3.3 20,717 + -0.5 20,718 - -0.0 20,755 + -2.0 20,763 - +0.6 20,785 - +0.7 20,800 - -1.1 20,802 - +0.2 20,827 + -0.8 20,899 + +0.5 20,899 + +0.2 20,900 - +0.8 20,900 - -2.2 20,987 + -0.9 20,987 + +0.0 20,988 - +0.6 20,988 - -0.2 20,988 - -1.2 21,010 + -0.5 21,053 + -0.4 21,054 - -2.7 21,054 - -0.3 21,054 - +0.9 21,054 - +0.2 21,054 - +0.5 21,069 + +0.9 21,093 + -1.4 21,093 + +1.8 21,093 + -0.3 21,093 + -1.4 21,093 + -1.4 21,149 + pepD MCAODC_00220 0.11 -0.5 21,149 + pepD MCAODC_00220 0.11 -0.8 21,149 + pepD MCAODC_00220 0.11 -1.9 21,149 + pepD MCAODC_00220 0.11 +0.2 21,150 - pepD MCAODC_00220 0.11 +1.5 21,150 - pepD MCAODC_00220 0.11 +0.5 21,152 + pepD MCAODC_00220 0.11 -1.8 21,152 + pepD MCAODC_00220 0.11 +1.5 21,153 - pepD MCAODC_00220 0.11 -0.1 21,182 + pepD MCAODC_00220 0.13 +0.2 21,183 - pepD MCAODC_00220 0.13 -0.6 21,194 + pepD MCAODC_00220 0.14 -1.0 21,242 + pepD MCAODC_00220 0.17 +0.3 21,276 - pepD MCAODC_00220 0.20 -0.7 21,285 + pepD MCAODC_00220 0.20 -2.3 21,286 - pepD MCAODC_00220 0.20 +0.4 21,286 - pepD MCAODC_00220 0.20 -0.4 21,287 + pepD MCAODC_00220 0.20 -2.4 21,287 + pepD MCAODC_00220 0.20 -0.2 21,287 + pepD MCAODC_00220 0.20 +0.1 21,287 + pepD MCAODC_00220 0.20 +0.2 21,287 + pepD MCAODC_00220 0.20 -0.6 21,287 + pepD MCAODC_00220 0.20 +0.7 21,287 + pepD MCAODC_00220 0.20 +0.6 21,287 + pepD MCAODC_00220 0.20 +0.7 21,288 - pepD MCAODC_00220 0.20 +0.8 21,288 - pepD MCAODC_00220 0.20 +0.5 21,288 - pepD MCAODC_00220 0.20 -1.1 21,288 - pepD MCAODC_00220 0.20 +0.2 21,288 - pepD MCAODC_00220 0.20 +0.8 21,288 - pepD MCAODC_00220 0.20 +0.6 21,288 - pepD MCAODC_00220 0.20 -1.4 21,347 + pepD MCAODC_00220 0.24 +0.4 21,488 + pepD MCAODC_00220 0.34 +1.0 21,488 + pepD MCAODC_00220 0.34 -1.2 21,519 + pepD MCAODC_00220 0.36 -2.1 21,519 + pepD MCAODC_00220 0.36 -0.3 21,519 + pepD MCAODC_00220 0.36 -0.8 21,566 - pepD MCAODC_00220 0.39 +0.3 21,613 + pepD MCAODC_00220 0.43 +0.3 21,613 + pepD MCAODC_00220 0.43 +0.3 21,614 - pepD MCAODC_00220 0.43 -0.0 21,614 - pepD MCAODC_00220 0.43 -0.5 21,614 - pepD MCAODC_00220 0.43 +0.8 21,834 - pepD MCAODC_00220 0.58 +0.8 21,834 - pepD MCAODC_00220 0.58 -0.9 21,834 - pepD MCAODC_00220 0.58 +0.6 21,834 - pepD MCAODC_00220 0.58 +0.8 21,837 + pepD MCAODC_00220 0.58 -1.2 21,838 - pepD MCAODC_00220 0.58 -0.3 21,838 - pepD MCAODC_00220 0.58 +0.8 21,838 - pepD MCAODC_00220 0.58 +0.5 21,838 - pepD MCAODC_00220 0.58 -1.1 21,914 + pepD MCAODC_00220 0.63 -0.5 21,914 + pepD MCAODC_00220 0.63 +0.2 21,951 - pepD MCAODC_00220 0.66 -0.3 22,063 + pepD MCAODC_00220 0.74 +1.1 22,110 + pepD MCAODC_00220 0.77 +0.3 22,111 - pepD MCAODC_00220 0.77 +0.7 22,111 - pepD MCAODC_00220 0.77 -1.2 22,111 - pepD MCAODC_00220 0.77 +0.2 22,176 + pepD MCAODC_00220 0.81 -0.2 22,222 + pepD MCAODC_00220 0.84 -0.7 22,334 + +0.7 22,393 + +0.7 22,393 + +1.6 22,393 + +1.4 22,394 - -0.1 22,447 - -0.5 22,451 - -1.5 22,517 - -0.5 22,603 + prfH MCAODC_00225 0.20 +0.7 22,604 - prfH MCAODC_00225 0.20 -2.2 22,604 - prfH MCAODC_00225 0.20 +0.8 22,664 - prfH MCAODC_00225 0.32 +0.5 22,728 - prfH MCAODC_00225 0.45 -0.8 22,825 + prfH MCAODC_00225 0.64 -0.4 22,825 + prfH MCAODC_00225 0.64 +0.6 22,981 - +0.2 23,085 + ykfJ MCAODC_00230 0.42 +0.8 23,103 + ykfJ MCAODC_00230 0.48 +0.3 23,140 - ykfJ MCAODC_00230 0.62 -0.7 23,189 + ykfJ MCAODC_00230 0.81 +1.4 23,190 - ykfJ MCAODC_00230 0.81 +0.6 23,190 - ykfJ MCAODC_00230 0.81 -3.4 23,194 - ykfJ MCAODC_00230 0.82 -0.5 23,216 + +0.5 23,217 - -2.0 23,218 + +1.2 23,219 - +1.3 23,231 - +0.1 23,242 + -1.1 23,242 + -0.8 23,242 + -1.8 23,243 - -0.9 23,254 - -0.3 23,254 - -0.3 23,306 - -0.4 23,310 - +0.5 23,369 + -1.9
Or see this region's nucleotide sequence