Experiment: Biofilm; Day2; SGW; pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18215 and LRK53_RS18220 are separated by 73 nucleotides LRK53_RS18220 and LRK53_RS18225 are separated by 6 nucleotides LRK53_RS18225 and LRK53_RS18230 are separated by 451 nucleotides
LRK53_RS18215: LRK53_RS18215 - single-stranded DNA-binding protein, at 19,257 to 19,847
_RS18215
LRK53_RS18220: LRK53_RS18220 - hypothetical protein, at 19,921 to 20,070
_RS18220
LRK53_RS18225: LRK53_RS18225 - DEAD/DEAH box helicase, at 20,077 to 22,062
_RS18225
LRK53_RS18230: LRK53_RS18230 - sel1 repeat family protein, at 22,514 to 23,089
_RS18230
Position (kb)
20
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 19.231 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.232 kb on - strand at 19.232 kb on - strand at 19.238 kb on + strand at 19.239 kb on - strand at 19.239 kb on - strand at 19.256 kb on + strand at 19.256 kb on + strand at 19.256 kb on + strand at 19.257 kb on - strand at 19.286 kb on + strand at 19.286 kb on + strand at 19.286 kb on + strand at 19.287 kb on - strand at 19.287 kb on - strand at 19.467 kb on + strand, within LRK53_RS18215 at 19.467 kb on + strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.468 kb on - strand, within LRK53_RS18215 at 19.491 kb on + strand, within LRK53_RS18215 at 19.492 kb on - strand, within LRK53_RS18215 at 19.492 kb on - strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.521 kb on + strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.522 kb on - strand, within LRK53_RS18215 at 19.723 kb on - strand, within LRK53_RS18215 at 19.835 kb on + strand at 19.836 kb on - strand at 19.836 kb on - strand at 19.838 kb on - strand at 19.845 kb on + strand at 19.869 kb on - strand at 19.869 kb on - strand at 19.870 kb on + strand at 19.893 kb on - strand at 20.068 kb on + strand at 20.069 kb on - strand at 20.179 kb on + strand at 20.179 kb on + strand at 20.180 kb on - strand at 20.494 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.545 kb on + strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.546 kb on - strand, within LRK53_RS18225 at 20.579 kb on - strand, within LRK53_RS18225 at 20.699 kb on - strand, within LRK53_RS18225 at 20.699 kb on - strand, within LRK53_RS18225 at 20.722 kb on + strand, within LRK53_RS18225 at 21.016 kb on + strand, within LRK53_RS18225 at 21.017 kb on - strand, within LRK53_RS18225 at 21.146 kb on + strand, within LRK53_RS18225 at 21.146 kb on + strand, within LRK53_RS18225 at 21.226 kb on + strand, within LRK53_RS18225 at 21.226 kb on + strand, within LRK53_RS18225 at 21.226 kb on + strand, within LRK53_RS18225 at 21.226 kb on + strand, within LRK53_RS18225 at 21.227 kb on - strand, within LRK53_RS18225 at 21.227 kb on - strand, within LRK53_RS18225 at 21.227 kb on - strand, within LRK53_RS18225 at 21.227 kb on - strand, within LRK53_RS18225 at 21.227 kb on - strand, within LRK53_RS18225 at 21.248 kb on + strand, within LRK53_RS18225 at 21.248 kb on + strand, within LRK53_RS18225 at 21.248 kb on + strand, within LRK53_RS18225 at 21.248 kb on + strand, within LRK53_RS18225 at 21.248 kb on + strand, within LRK53_RS18225 at 21.249 kb on - strand, within LRK53_RS18225 at 21.249 kb on - strand, within LRK53_RS18225 at 21.249 kb on - strand, within LRK53_RS18225 at 21.251 kb on + strand, within LRK53_RS18225 at 21.251 kb on + strand, within LRK53_RS18225 at 21.251 kb on + strand, within LRK53_RS18225 at 21.251 kb on + strand, within LRK53_RS18225 at 21.251 kb on + strand, within LRK53_RS18225 at 21.252 kb on - strand, within LRK53_RS18225 at 21.252 kb on - strand, within LRK53_RS18225 at 21.252 kb on - strand, within LRK53_RS18225 at 21.252 kb on - strand, within LRK53_RS18225 at 21.252 kb on - strand, within LRK53_RS18225 at 21.354 kb on + strand, within LRK53_RS18225 at 21.354 kb on + strand, within LRK53_RS18225 at 21.354 kb on + strand, within LRK53_RS18225 at 21.354 kb on + strand, within LRK53_RS18225 at 21.354 kb on + strand, within LRK53_RS18225 at 21.355 kb on - strand, within LRK53_RS18225 at 21.355 kb on - strand, within LRK53_RS18225 at 21.355 kb on - strand, within LRK53_RS18225 at 21.355 kb on - strand, within LRK53_RS18225 at 21.440 kb on - strand, within LRK53_RS18225 at 21.440 kb on - strand, within LRK53_RS18225 at 21.562 kb on + strand, within LRK53_RS18225 at 21.563 kb on - strand, within LRK53_RS18225 at 21.563 kb on - strand, within LRK53_RS18225 at 21.563 kb on - strand, within LRK53_RS18225 at 21.667 kb on - strand, within LRK53_RS18225 at 21.667 kb on - strand, within LRK53_RS18225 at 21.667 kb on - strand, within LRK53_RS18225 at 21.704 kb on - strand, within LRK53_RS18225 at 21.965 kb on + strand at 21.966 kb on - strand at 21.966 kb on - strand at 22.091 kb on + strand at 22.091 kb on + strand at 22.117 kb on + strand at 22.261 kb on + strand at 22.290 kb on + strand at 22.290 kb on + strand at 22.290 kb on + strand at 22.290 kb on + strand at 22.291 kb on - strand at 22.291 kb on - strand at 22.291 kb on - strand at 22.291 kb on - strand at 22.291 kb on - strand at 22.324 kb on - strand at 22.324 kb on - strand at 22.453 kb on + strand at 22.453 kb on + strand at 22.453 kb on + strand at 22.453 kb on + strand at 22.453 kb on + strand at 22.454 kb on - strand at 22.454 kb on - strand at 22.454 kb on - strand at 22.455 kb on + strand at 22.455 kb on + strand at 22.456 kb on - strand at 22.456 kb on - strand at 22.594 kb on + strand, within LRK53_RS18230 at 22.617 kb on + strand, within LRK53_RS18230 at 22.617 kb on + strand, within LRK53_RS18230 at 22.618 kb on - strand, within LRK53_RS18230 at 22.618 kb on - strand, within LRK53_RS18230 at 22.756 kb on - strand, within LRK53_RS18230 at 22.856 kb on + strand, within LRK53_RS18230 at 22.856 kb on + strand, within LRK53_RS18230 at 22.856 kb on + strand, within LRK53_RS18230 at 22.856 kb on + strand, within LRK53_RS18230 at 22.856 kb on + strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.857 kb on - strand, within LRK53_RS18230 at 22.962 kb on + strand, within LRK53_RS18230 at 22.962 kb on + strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.963 kb on - strand, within LRK53_RS18230 at 22.970 kb on + strand, within LRK53_RS18230 at 23.018 kb on + strand, within LRK53_RS18230 at 23.018 kb on + strand, within LRK53_RS18230 at 23.019 kb on - strand, within LRK53_RS18230 at 23.019 kb on - strand, within LRK53_RS18230
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm; Day2; SGW; pH7 remove 19,231 + -2.1 19,231 + +0.6 19,231 + -1.7 19,232 - -1.2 19,232 - -0.4 19,238 + -0.1 19,239 - -0.7 19,239 - -0.4 19,256 + -0.9 19,256 + -0.6 19,256 + +1.2 19,257 - +0.7 19,286 + -1.1 19,286 + -0.6 19,286 + -2.0 19,287 - -1.1 19,287 - -1.7 19,467 + LRK53_RS18215 0.36 +0.6 19,467 + LRK53_RS18215 0.36 -0.7 19,468 - LRK53_RS18215 0.36 -1.9 19,468 - LRK53_RS18215 0.36 -1.6 19,468 - LRK53_RS18215 0.36 +0.5 19,491 + LRK53_RS18215 0.40 -2.8 19,492 - LRK53_RS18215 0.40 -0.5 19,492 - LRK53_RS18215 0.40 +1.1 19,521 + LRK53_RS18215 0.45 -1.5 19,521 + LRK53_RS18215 0.45 -1.3 19,521 + LRK53_RS18215 0.45 +0.4 19,521 + LRK53_RS18215 0.45 -0.3 19,521 + LRK53_RS18215 0.45 -0.3 19,522 - LRK53_RS18215 0.45 -0.1 19,522 - LRK53_RS18215 0.45 -0.0 19,522 - LRK53_RS18215 0.45 -0.1 19,522 - LRK53_RS18215 0.45 -0.6 19,522 - LRK53_RS18215 0.45 -0.4 19,522 - LRK53_RS18215 0.45 +0.1 19,522 - LRK53_RS18215 0.45 -0.4 19,723 - LRK53_RS18215 0.79 -1.3 19,835 + -0.8 19,836 - -0.7 19,836 - +0.5 19,838 - -1.5 19,845 + +0.6 19,869 - -1.9 19,869 - -1.1 19,870 + -0.7 19,893 - -0.8 20,068 + -1.5 20,069 - -0.5 20,179 + -0.8 20,179 + +0.7 20,180 - +0.7 20,494 + LRK53_RS18225 0.21 -0.2 20,545 + LRK53_RS18225 0.24 -1.3 20,545 + LRK53_RS18225 0.24 -0.0 20,545 + LRK53_RS18225 0.24 +0.2 20,545 + LRK53_RS18225 0.24 -1.4 20,545 + LRK53_RS18225 0.24 -0.7 20,545 + LRK53_RS18225 0.24 -0.5 20,545 + LRK53_RS18225 0.24 +0.3 20,546 - LRK53_RS18225 0.24 -0.1 20,546 - LRK53_RS18225 0.24 +0.7 20,546 - LRK53_RS18225 0.24 +1.1 20,546 - LRK53_RS18225 0.24 -1.8 20,579 - LRK53_RS18225 0.25 -0.6 20,699 - LRK53_RS18225 0.31 -2.4 20,699 - LRK53_RS18225 0.31 -0.2 20,722 + LRK53_RS18225 0.32 -1.2 21,016 + LRK53_RS18225 0.47 -0.6 21,017 - LRK53_RS18225 0.47 -0.2 21,146 + LRK53_RS18225 0.54 +0.5 21,146 + LRK53_RS18225 0.54 -1.1 21,226 + LRK53_RS18225 0.58 -1.6 21,226 + LRK53_RS18225 0.58 +1.1 21,226 + LRK53_RS18225 0.58 -1.9 21,226 + LRK53_RS18225 0.58 +0.1 21,227 - LRK53_RS18225 0.58 -0.1 21,227 - LRK53_RS18225 0.58 -2.5 21,227 - LRK53_RS18225 0.58 +0.2 21,227 - LRK53_RS18225 0.58 -0.8 21,227 - LRK53_RS18225 0.58 +0.5 21,248 + LRK53_RS18225 0.59 +0.5 21,248 + LRK53_RS18225 0.59 -1.3 21,248 + LRK53_RS18225 0.59 -0.0 21,248 + LRK53_RS18225 0.59 -0.5 21,248 + LRK53_RS18225 0.59 -0.8 21,249 - LRK53_RS18225 0.59 -1.6 21,249 - LRK53_RS18225 0.59 -1.4 21,249 - LRK53_RS18225 0.59 -2.9 21,251 + LRK53_RS18225 0.59 -0.7 21,251 + LRK53_RS18225 0.59 -1.2 21,251 + LRK53_RS18225 0.59 -0.7 21,251 + LRK53_RS18225 0.59 +0.1 21,251 + LRK53_RS18225 0.59 +0.5 21,252 - LRK53_RS18225 0.59 -1.9 21,252 - LRK53_RS18225 0.59 -1.7 21,252 - LRK53_RS18225 0.59 -2.1 21,252 - LRK53_RS18225 0.59 +0.2 21,252 - LRK53_RS18225 0.59 +2.7 21,354 + LRK53_RS18225 0.64 -1.5 21,354 + LRK53_RS18225 0.64 -0.5 21,354 + LRK53_RS18225 0.64 -2.6 21,354 + LRK53_RS18225 0.64 -1.0 21,354 + LRK53_RS18225 0.64 -0.5 21,355 - LRK53_RS18225 0.64 -0.0 21,355 - LRK53_RS18225 0.64 -1.6 21,355 - LRK53_RS18225 0.64 -0.3 21,355 - LRK53_RS18225 0.64 -0.1 21,440 - LRK53_RS18225 0.69 -1.3 21,440 - LRK53_RS18225 0.69 +0.8 21,562 + LRK53_RS18225 0.75 -1.5 21,563 - LRK53_RS18225 0.75 -0.4 21,563 - LRK53_RS18225 0.75 -0.1 21,563 - LRK53_RS18225 0.75 -2.5 21,667 - LRK53_RS18225 0.80 -0.7 21,667 - LRK53_RS18225 0.80 -1.1 21,667 - LRK53_RS18225 0.80 +0.1 21,704 - LRK53_RS18225 0.82 -1.7 21,965 + +0.2 21,966 - -1.3 21,966 - +0.9 22,091 + -1.3 22,091 + -1.4 22,117 + -1.5 22,261 + -1.5 22,290 + -0.2 22,290 + -1.6 22,290 + -1.4 22,290 + -1.6 22,291 - -0.4 22,291 - -2.4 22,291 - -2.6 22,291 - +0.2 22,291 - -1.4 22,324 - -0.7 22,324 - +0.4 22,453 + -0.4 22,453 + -1.5 22,453 + -0.2 22,453 + -0.9 22,453 + -1.3 22,454 - -0.8 22,454 - -2.4 22,454 - -1.6 22,455 + -0.3 22,455 + -0.1 22,456 - -1.5 22,456 - -1.3 22,594 + LRK53_RS18230 0.14 -1.3 22,617 + LRK53_RS18230 0.18 -0.7 22,617 + LRK53_RS18230 0.18 -1.6 22,618 - LRK53_RS18230 0.18 -1.1 22,618 - LRK53_RS18230 0.18 +0.1 22,756 - LRK53_RS18230 0.42 -2.3 22,856 + LRK53_RS18230 0.59 -2.6 22,856 + LRK53_RS18230 0.59 -0.2 22,856 + LRK53_RS18230 0.59 +0.3 22,856 + LRK53_RS18230 0.59 -1.2 22,856 + LRK53_RS18230 0.59 -1.8 22,857 - LRK53_RS18230 0.60 -0.1 22,857 - LRK53_RS18230 0.60 -1.1 22,857 - LRK53_RS18230 0.60 +0.2 22,857 - LRK53_RS18230 0.60 -1.2 22,857 - LRK53_RS18230 0.60 -1.8 22,857 - LRK53_RS18230 0.60 -0.8 22,857 - LRK53_RS18230 0.60 -0.2 22,857 - LRK53_RS18230 0.60 -1.7 22,857 - LRK53_RS18230 0.60 +0.6 22,962 + LRK53_RS18230 0.78 -0.8 22,962 + LRK53_RS18230 0.78 -0.1 22,963 - LRK53_RS18230 0.78 +0.9 22,963 - LRK53_RS18230 0.78 -1.5 22,963 - LRK53_RS18230 0.78 -0.5 22,963 - LRK53_RS18230 0.78 +0.2 22,963 - LRK53_RS18230 0.78 -0.3 22,963 - LRK53_RS18230 0.78 -0.4 22,970 + LRK53_RS18230 0.79 -1.2 23,018 + LRK53_RS18230 0.88 -0.3 23,018 + LRK53_RS18230 0.88 -0.8 23,019 - LRK53_RS18230 0.88 -2.3 23,019 - LRK53_RS18230 0.88 +0.5
Or see this region's nucleotide sequence