Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pbp4b and murP are separated by 4 nucleotides murP and murQ are separated by 3 nucleotides
HEPCGN_21300: pbp4b - penicillin binding protein PBP4B, at 3,012,337 to 3,013,638
pbp4b
HEPCGN_21305: murP - PTS N-acetylmuramic acid transporter subunit IIBC, at 3,013,643 to 3,015,067
murP
HEPCGN_21310: murQ - N-acetylmuramic acid 6-phosphate etherase, at 3,015,071 to 3,015,967
murQ
Position (kb)
3013
3014
3015
3016 Strain fitness (log2 ratio)
-2
-1
0
1 at 3012.681 kb on - strand, within pbp4b at 3012.891 kb on + strand, within pbp4b at 3013.014 kb on + strand, within pbp4b at 3013.015 kb on - strand, within pbp4b at 3013.016 kb on + strand, within pbp4b at 3013.016 kb on + strand, within pbp4b at 3013.017 kb on - strand, within pbp4b at 3013.035 kb on + strand, within pbp4b at 3013.035 kb on + strand, within pbp4b at 3013.036 kb on - strand, within pbp4b at 3013.036 kb on - strand, within pbp4b at 3013.311 kb on + strand, within pbp4b at 3013.311 kb on + strand, within pbp4b at 3013.311 kb on + strand, within pbp4b at 3013.311 kb on + strand, within pbp4b at 3013.347 kb on - strand, within pbp4b at 3013.347 kb on - strand, within pbp4b at 3013.385 kb on - strand, within pbp4b at 3013.400 kb on + strand, within pbp4b at 3013.452 kb on + strand, within pbp4b at 3013.452 kb on + strand, within pbp4b at 3013.453 kb on - strand, within pbp4b at 3013.465 kb on + strand, within pbp4b at 3013.593 kb on + strand at 3013.808 kb on + strand, within murP at 3014.063 kb on + strand, within murP at 3014.063 kb on + strand, within murP at 3014.064 kb on - strand, within murP at 3014.064 kb on - strand, within murP at 3014.101 kb on - strand, within murP at 3014.133 kb on - strand, within murP at 3014.182 kb on + strand, within murP at 3014.198 kb on - strand, within murP at 3014.212 kb on + strand, within murP at 3014.213 kb on - strand, within murP at 3014.242 kb on + strand, within murP at 3014.242 kb on + strand, within murP at 3014.242 kb on + strand, within murP at 3014.436 kb on + strand, within murP at 3014.437 kb on - strand, within murP at 3014.458 kb on + strand, within murP at 3014.460 kb on + strand, within murP at 3014.460 kb on + strand, within murP at 3014.533 kb on - strand, within murP at 3014.533 kb on - strand, within murP at 3014.773 kb on + strand, within murP at 3014.774 kb on - strand, within murP at 3014.805 kb on - strand, within murP at 3014.857 kb on + strand, within murP at 3014.874 kb on - strand, within murP at 3014.874 kb on - strand, within murP at 3014.957 kb on + strand at 3014.958 kb on - strand at 3014.980 kb on + strand at 3014.981 kb on - strand at 3014.986 kb on + strand at 3015.197 kb on + strand, within murQ at 3015.198 kb on - strand, within murQ at 3015.224 kb on - strand, within murQ at 3015.248 kb on - strand, within murQ at 3015.325 kb on + strand, within murQ at 3015.349 kb on + strand, within murQ at 3015.416 kb on + strand, within murQ at 3015.417 kb on - strand, within murQ at 3015.417 kb on - strand, within murQ at 3015.531 kb on + strand, within murQ at 3015.531 kb on + strand, within murQ at 3015.531 kb on + strand, within murQ at 3015.768 kb on + strand, within murQ at 3015.947 kb on - strand at 3015.981 kb on - strand at 3015.981 kb on - strand at 3015.984 kb on - strand at 3015.998 kb on - strand at 3016.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 3,012,681 - pbp4b HEPCGN_21300 0.26 +0.3 3,012,891 + pbp4b HEPCGN_21300 0.43 +0.1 3,013,014 + pbp4b HEPCGN_21300 0.52 -0.3 3,013,015 - pbp4b HEPCGN_21300 0.52 -0.0 3,013,016 + pbp4b HEPCGN_21300 0.52 +0.6 3,013,016 + pbp4b HEPCGN_21300 0.52 +0.5 3,013,017 - pbp4b HEPCGN_21300 0.52 +0.6 3,013,035 + pbp4b HEPCGN_21300 0.54 +1.0 3,013,035 + pbp4b HEPCGN_21300 0.54 +0.1 3,013,036 - pbp4b HEPCGN_21300 0.54 +0.3 3,013,036 - pbp4b HEPCGN_21300 0.54 +0.2 3,013,311 + pbp4b HEPCGN_21300 0.75 +0.3 3,013,311 + pbp4b HEPCGN_21300 0.75 +0.1 3,013,311 + pbp4b HEPCGN_21300 0.75 +0.3 3,013,311 + pbp4b HEPCGN_21300 0.75 +0.8 3,013,347 - pbp4b HEPCGN_21300 0.78 +0.2 3,013,347 - pbp4b HEPCGN_21300 0.78 +1.0 3,013,385 - pbp4b HEPCGN_21300 0.80 -0.6 3,013,400 + pbp4b HEPCGN_21300 0.82 +1.1 3,013,452 + pbp4b HEPCGN_21300 0.86 +0.8 3,013,452 + pbp4b HEPCGN_21300 0.86 +1.2 3,013,453 - pbp4b HEPCGN_21300 0.86 +0.2 3,013,465 + pbp4b HEPCGN_21300 0.87 +0.0 3,013,593 + +0.2 3,013,808 + murP HEPCGN_21305 0.12 +0.1 3,014,063 + murP HEPCGN_21305 0.29 +0.5 3,014,063 + murP HEPCGN_21305 0.29 +0.1 3,014,064 - murP HEPCGN_21305 0.30 +1.3 3,014,064 - murP HEPCGN_21305 0.30 -0.4 3,014,101 - murP HEPCGN_21305 0.32 -2.1 3,014,133 - murP HEPCGN_21305 0.34 +0.3 3,014,182 + murP HEPCGN_21305 0.38 +0.4 3,014,198 - murP HEPCGN_21305 0.39 -0.0 3,014,212 + murP HEPCGN_21305 0.40 +0.5 3,014,213 - murP HEPCGN_21305 0.40 +0.4 3,014,242 + murP HEPCGN_21305 0.42 +0.0 3,014,242 + murP HEPCGN_21305 0.42 +0.2 3,014,242 + murP HEPCGN_21305 0.42 +0.5 3,014,436 + murP HEPCGN_21305 0.56 +0.6 3,014,437 - murP HEPCGN_21305 0.56 -0.6 3,014,458 + murP HEPCGN_21305 0.57 +0.0 3,014,460 + murP HEPCGN_21305 0.57 -0.3 3,014,460 + murP HEPCGN_21305 0.57 -0.8 3,014,533 - murP HEPCGN_21305 0.62 +0.1 3,014,533 - murP HEPCGN_21305 0.62 +0.7 3,014,773 + murP HEPCGN_21305 0.79 +0.1 3,014,774 - murP HEPCGN_21305 0.79 +0.7 3,014,805 - murP HEPCGN_21305 0.82 -0.1 3,014,857 + murP HEPCGN_21305 0.85 +0.0 3,014,874 - murP HEPCGN_21305 0.86 +0.3 3,014,874 - murP HEPCGN_21305 0.86 +0.4 3,014,957 + +1.2 3,014,958 - +0.1 3,014,980 + +0.2 3,014,981 - +0.4 3,014,986 + +0.1 3,015,197 + murQ HEPCGN_21310 0.14 -0.1 3,015,198 - murQ HEPCGN_21310 0.14 +0.4 3,015,224 - murQ HEPCGN_21310 0.17 +0.0 3,015,248 - murQ HEPCGN_21310 0.20 +0.1 3,015,325 + murQ HEPCGN_21310 0.28 +0.2 3,015,349 + murQ HEPCGN_21310 0.31 +0.5 3,015,416 + murQ HEPCGN_21310 0.38 +0.5 3,015,417 - murQ HEPCGN_21310 0.39 -0.1 3,015,417 - murQ HEPCGN_21310 0.39 -0.2 3,015,531 + murQ HEPCGN_21310 0.51 -0.9 3,015,531 + murQ HEPCGN_21310 0.51 +0.2 3,015,531 + murQ HEPCGN_21310 0.51 -0.1 3,015,768 + murQ HEPCGN_21310 0.78 -0.5 3,015,947 - +1.6 3,015,981 - -0.1 3,015,981 - -0.2 3,015,984 - -0.2 3,015,998 - -0.3 3,016,040 + -1.0
Or see this region's nucleotide sequence