Strain Fitness in Escherichia coli ECOR38 around HEPCGN_21220

Experiment: Bas39

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutE and eutJ are separated by 10 nucleotideseutJ and eutG overlap by 11 nucleotides HEPCGN_21215: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 2,997,064 to 2,998,467 eutE HEPCGN_21220: eutJ - ethanolamine utilization protein EutJ, at 2,998,478 to 2,999,314 eutJ HEPCGN_21225: eutG - ethanolamine utilization ethanol dehydrogenase EutG, at 2,999,304 to 3,000,491 eutG Position (kb) 2998 2999 3000Strain fitness (log2 ratio) -1 0 1 2at 2997.493 kb on - strand, within eutEat 2997.493 kb on - strand, within eutEat 2997.725 kb on + strand, within eutEat 2997.726 kb on - strand, within eutEat 2997.751 kb on - strand, within eutEat 2997.884 kb on + strand, within eutEat 2998.113 kb on - strand, within eutEat 2998.440 kb on - strandat 2998.442 kb on + strandat 2998.798 kb on + strand, within eutJat 2999.020 kb on + strand, within eutJat 2999.174 kb on - strand, within eutJat 2999.174 kb on - strand, within eutJat 2999.302 kb on - strandat 2999.302 kb on - strandat 2999.304 kb on - strandat 2999.375 kb on + strandat 2999.375 kb on + strandat 2999.376 kb on - strandat 2999.376 kb on - strandat 2999.376 kb on - strandat 2999.516 kb on + strand, within eutGat 2999.703 kb on - strand, within eutGat 2999.814 kb on - strand, within eutGat 2999.845 kb on + strand, within eutGat 2999.964 kb on + strand, within eutGat 2999.964 kb on + strand, within eutGat 3000.054 kb on + strand, within eutGat 3000.101 kb on + strand, within eutGat 3000.102 kb on - strand, within eutGat 3000.102 kb on - strand, within eutGat 3000.179 kb on + strand, within eutGat 3000.203 kb on + strand, within eutGat 3000.203 kb on + strand, within eutGat 3000.204 kb on - strand, within eutGat 3000.204 kb on - strand, within eutGat 3000.231 kb on - strand, within eutGat 3000.308 kb on + strand, within eutGat 3000.309 kb on - strand, within eutG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas39
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2,997,493 - eutE HEPCGN_21215 0.31 +0.2
2,997,493 - eutE HEPCGN_21215 0.31 +1.8
2,997,725 + eutE HEPCGN_21215 0.47 +0.8
2,997,726 - eutE HEPCGN_21215 0.47 +0.1
2,997,751 - eutE HEPCGN_21215 0.49 -0.5
2,997,884 + eutE HEPCGN_21215 0.58 -0.8
2,998,113 - eutE HEPCGN_21215 0.75 -0.3
2,998,440 - +0.3
2,998,442 + +0.5
2,998,798 + eutJ HEPCGN_21220 0.38 +0.3
2,999,020 + eutJ HEPCGN_21220 0.65 +0.6
2,999,174 - eutJ HEPCGN_21220 0.83 +1.7
2,999,174 - eutJ HEPCGN_21220 0.83 +0.4
2,999,302 - -0.7
2,999,302 - -0.8
2,999,304 - -0.1
2,999,375 + -0.1
2,999,375 + +0.6
2,999,376 - -0.4
2,999,376 - +0.0
2,999,376 - -0.1
2,999,516 + eutG HEPCGN_21225 0.18 +0.3
2,999,703 - eutG HEPCGN_21225 0.34 -1.6
2,999,814 - eutG HEPCGN_21225 0.43 -0.2
2,999,845 + eutG HEPCGN_21225 0.46 +0.7
2,999,964 + eutG HEPCGN_21225 0.56 +0.0
2,999,964 + eutG HEPCGN_21225 0.56 +0.0
3,000,054 + eutG HEPCGN_21225 0.63 +0.4
3,000,101 + eutG HEPCGN_21225 0.67 -0.1
3,000,102 - eutG HEPCGN_21225 0.67 +0.1
3,000,102 - eutG HEPCGN_21225 0.67 +0.3
3,000,179 + eutG HEPCGN_21225 0.74 +0.1
3,000,203 + eutG HEPCGN_21225 0.76 -0.5
3,000,203 + eutG HEPCGN_21225 0.76 +0.2
3,000,204 - eutG HEPCGN_21225 0.76 -0.3
3,000,204 - eutG HEPCGN_21225 0.76 +0.9
3,000,231 - eutG HEPCGN_21225 0.78 +0.5
3,000,308 + eutG HEPCGN_21225 0.85 +0.3
3,000,309 - eutG HEPCGN_21225 0.85 +0.4

Or see this region's nucleotide sequence