Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speB and loiP are separated by 47 nucleotides loiP and tkt are separated by 277 nucleotides
HEPCGN_18620: speB - agmatinase, at 2,468,620 to 2,469,540
speB
HEPCGN_18625: loiP - metalloprotease LoiP, at 2,469,588 to 2,470,346
loiP
HEPCGN_18630: tkt - transketolase, at 2,470,624 to 2,472,615
tkt
Position (kb)
2469
2470
2471 Strain fitness (log2 ratio)
-2
-1
0
1 at 2468.607 kb on + strand at 2468.607 kb on + strand at 2468.607 kb on + strand at 2468.630 kb on + strand at 2468.641 kb on + strand at 2468.896 kb on + strand, within speB at 2468.897 kb on - strand, within speB at 2468.897 kb on - strand, within speB at 2468.973 kb on + strand, within speB at 2468.974 kb on - strand, within speB at 2469.004 kb on - strand, within speB at 2469.079 kb on - strand, within speB at 2469.079 kb on - strand, within speB at 2469.121 kb on + strand, within speB at 2469.121 kb on + strand, within speB at 2469.285 kb on + strand, within speB at 2469.285 kb on + strand, within speB at 2469.356 kb on + strand, within speB at 2469.384 kb on + strand, within speB at 2469.511 kb on + strand at 2469.511 kb on + strand at 2469.511 kb on + strand at 2469.512 kb on - strand at 2469.514 kb on - strand at 2469.538 kb on + strand at 2469.539 kb on - strand at 2469.549 kb on + strand at 2469.550 kb on - strand at 2469.580 kb on + strand at 2469.594 kb on - strand at 2469.594 kb on - strand at 2469.594 kb on - strand at 2469.594 kb on - strand at 2469.611 kb on + strand at 2469.624 kb on - strand at 2469.806 kb on + strand, within loiP at 2469.811 kb on - strand, within loiP at 2469.811 kb on - strand, within loiP at 2469.900 kb on - strand, within loiP at 2469.984 kb on - strand, within loiP at 2469.995 kb on + strand, within loiP at 2470.065 kb on - strand, within loiP at 2470.067 kb on - strand, within loiP at 2470.067 kb on - strand, within loiP at 2470.067 kb on - strand, within loiP at 2470.067 kb on - strand, within loiP at 2470.075 kb on + strand, within loiP at 2470.097 kb on + strand, within loiP at 2470.098 kb on - strand, within loiP at 2470.098 kb on - strand, within loiP at 2470.121 kb on + strand, within loiP at 2470.121 kb on + strand, within loiP at 2470.122 kb on - strand, within loiP at 2470.122 kb on - strand, within loiP at 2470.141 kb on + strand, within loiP at 2470.421 kb on + strand at 2470.421 kb on + strand at 2470.423 kb on + strand at 2470.424 kb on - strand at 2470.512 kb on + strand at 2470.636 kb on - strand at 2470.720 kb on - strand at 2470.938 kb on + strand, within tkt at 2471.072 kb on - strand, within tkt at 2471.181 kb on + strand, within tkt at 2471.181 kb on + strand, within tkt
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 2,468,607 + -0.2 2,468,607 + -0.1 2,468,607 + -0.2 2,468,630 + +0.4 2,468,641 + -1.4 2,468,896 + speB HEPCGN_18620 0.30 -0.5 2,468,897 - speB HEPCGN_18620 0.30 -0.1 2,468,897 - speB HEPCGN_18620 0.30 -1.5 2,468,973 + speB HEPCGN_18620 0.38 -0.3 2,468,974 - speB HEPCGN_18620 0.38 +0.1 2,469,004 - speB HEPCGN_18620 0.42 +0.6 2,469,079 - speB HEPCGN_18620 0.50 -0.7 2,469,079 - speB HEPCGN_18620 0.50 -0.9 2,469,121 + speB HEPCGN_18620 0.54 -0.7 2,469,121 + speB HEPCGN_18620 0.54 -0.8 2,469,285 + speB HEPCGN_18620 0.72 -2.7 2,469,285 + speB HEPCGN_18620 0.72 -1.2 2,469,356 + speB HEPCGN_18620 0.80 -1.2 2,469,384 + speB HEPCGN_18620 0.83 -0.3 2,469,511 + -0.7 2,469,511 + +0.9 2,469,511 + -0.8 2,469,512 - -0.9 2,469,514 - +1.2 2,469,538 + -2.1 2,469,539 - +0.1 2,469,549 + +0.3 2,469,550 - +0.1 2,469,580 + -0.2 2,469,594 - +0.6 2,469,594 - +0.1 2,469,594 - -0.0 2,469,594 - +0.4 2,469,611 + +0.0 2,469,624 - -0.7 2,469,806 + loiP HEPCGN_18625 0.29 -0.2 2,469,811 - loiP HEPCGN_18625 0.29 -0.7 2,469,811 - loiP HEPCGN_18625 0.29 -0.6 2,469,900 - loiP HEPCGN_18625 0.41 -1.1 2,469,984 - loiP HEPCGN_18625 0.52 +1.4 2,469,995 + loiP HEPCGN_18625 0.54 +0.2 2,470,065 - loiP HEPCGN_18625 0.63 +0.5 2,470,067 - loiP HEPCGN_18625 0.63 -0.5 2,470,067 - loiP HEPCGN_18625 0.63 +0.5 2,470,067 - loiP HEPCGN_18625 0.63 -0.7 2,470,067 - loiP HEPCGN_18625 0.63 +0.3 2,470,075 + loiP HEPCGN_18625 0.64 +0.1 2,470,097 + loiP HEPCGN_18625 0.67 +0.1 2,470,098 - loiP HEPCGN_18625 0.67 -0.4 2,470,098 - loiP HEPCGN_18625 0.67 +0.5 2,470,121 + loiP HEPCGN_18625 0.70 +0.1 2,470,121 + loiP HEPCGN_18625 0.70 +0.7 2,470,122 - loiP HEPCGN_18625 0.70 +1.0 2,470,122 - loiP HEPCGN_18625 0.70 +0.7 2,470,141 + loiP HEPCGN_18625 0.73 +0.3 2,470,421 + +0.1 2,470,421 + +0.8 2,470,423 + -0.4 2,470,424 - -0.2 2,470,512 + -1.2 2,470,636 - -0.3 2,470,720 - -0.1 2,470,938 + tkt HEPCGN_18630 0.16 +1.0 2,471,072 - tkt HEPCGN_18630 0.22 -0.3 2,471,181 + tkt HEPCGN_18630 0.28 -0.2 2,471,181 + tkt HEPCGN_18630 0.28 -0.8
Or see this region's nucleotide sequence