Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceA and aceB are separated by 29 nucleotides aceB and metA are separated by 268 nucleotides
HEPCGN_11905: aceA - isocitrate lyase, at 1,119,653 to 1,120,957
aceA
HEPCGN_11910: aceB - malate synthase A, at 1,120,987 to 1,122,588
aceB
HEPCGN_11915: metA - homoserine O-succinyltransferase, at 1,122,857 to 1,123,786
metA
Position (kb)
1120
1121
1122
1123 Strain fitness (log2 ratio)
-2
-1
0
1 at 1120.010 kb on - strand, within aceA at 1120.011 kb on + strand, within aceA at 1120.011 kb on + strand, within aceA at 1120.011 kb on + strand, within aceA at 1120.100 kb on + strand, within aceA at 1120.113 kb on + strand, within aceA at 1120.114 kb on - strand, within aceA at 1120.114 kb on - strand, within aceA at 1120.201 kb on + strand, within aceA at 1120.215 kb on + strand, within aceA at 1120.331 kb on - strand, within aceA at 1120.331 kb on - strand, within aceA at 1120.510 kb on - strand, within aceA at 1120.518 kb on + strand, within aceA at 1120.579 kb on + strand, within aceA at 1120.693 kb on - strand, within aceA at 1120.764 kb on + strand, within aceA at 1120.853 kb on + strand at 1120.854 kb on - strand at 1120.879 kb on - strand at 1120.889 kb on - strand at 1120.926 kb on - strand at 1120.946 kb on - strand at 1120.972 kb on + strand at 1120.972 kb on + strand at 1120.972 kb on + strand at 1120.976 kb on + strand at 1120.976 kb on + strand at 1120.976 kb on + strand at 1120.976 kb on + strand at 1120.977 kb on - strand at 1120.977 kb on - strand at 1120.977 kb on - strand at 1120.988 kb on + strand at 1121.306 kb on + strand, within aceB at 1121.338 kb on - strand, within aceB at 1121.338 kb on - strand, within aceB at 1121.640 kb on + strand, within aceB at 1121.658 kb on - strand, within aceB at 1121.785 kb on - strand, within aceB at 1121.955 kb on - strand, within aceB at 1122.151 kb on - strand, within aceB at 1122.539 kb on + strand at 1122.540 kb on - strand at 1122.548 kb on - strand at 1122.668 kb on + strand at 1122.818 kb on - strand at 1122.884 kb on + strand at 1122.892 kb on - strand at 1122.907 kb on - strand at 1122.912 kb on + strand at 1122.912 kb on + strand at 1122.913 kb on - strand at 1122.913 kb on - strand at 1122.915 kb on + strand at 1122.916 kb on - strand at 1122.932 kb on - strand at 1122.999 kb on + strand, within metA at 1123.003 kb on + strand, within metA at 1123.003 kb on + strand, within metA at 1123.005 kb on + strand, within metA at 1123.006 kb on - strand, within metA at 1123.006 kb on - strand, within metA at 1123.129 kb on - strand, within metA at 1123.174 kb on - strand, within metA at 1123.274 kb on - strand, within metA at 1123.336 kb on + strand, within metA at 1123.336 kb on + strand, within metA at 1123.429 kb on - strand, within metA at 1123.475 kb on + strand, within metA at 1123.517 kb on - strand, within metA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 1,120,010 - aceA HEPCGN_11905 0.27 +0.0 1,120,011 + aceA HEPCGN_11905 0.27 +1.0 1,120,011 + aceA HEPCGN_11905 0.27 +0.5 1,120,011 + aceA HEPCGN_11905 0.27 -0.0 1,120,100 + aceA HEPCGN_11905 0.34 +0.3 1,120,113 + aceA HEPCGN_11905 0.35 -1.8 1,120,114 - aceA HEPCGN_11905 0.35 +1.0 1,120,114 - aceA HEPCGN_11905 0.35 +0.3 1,120,201 + aceA HEPCGN_11905 0.42 +1.3 1,120,215 + aceA HEPCGN_11905 0.43 +1.5 1,120,331 - aceA HEPCGN_11905 0.52 +0.2 1,120,331 - aceA HEPCGN_11905 0.52 +0.1 1,120,510 - aceA HEPCGN_11905 0.66 +0.3 1,120,518 + aceA HEPCGN_11905 0.66 +0.4 1,120,579 + aceA HEPCGN_11905 0.71 -0.0 1,120,693 - aceA HEPCGN_11905 0.80 -0.4 1,120,764 + aceA HEPCGN_11905 0.85 +0.7 1,120,853 + -0.0 1,120,854 - -0.0 1,120,879 - -0.2 1,120,889 - +0.0 1,120,926 - -2.3 1,120,946 - +0.4 1,120,972 + +0.4 1,120,972 + -0.3 1,120,972 + +0.4 1,120,976 + +0.4 1,120,976 + -0.2 1,120,976 + +0.6 1,120,976 + -0.4 1,120,977 - -0.1 1,120,977 - +0.2 1,120,977 - -0.6 1,120,988 + -0.0 1,121,306 + aceB HEPCGN_11910 0.20 +0.1 1,121,338 - aceB HEPCGN_11910 0.22 +0.8 1,121,338 - aceB HEPCGN_11910 0.22 +0.4 1,121,640 + aceB HEPCGN_11910 0.41 -0.6 1,121,658 - aceB HEPCGN_11910 0.42 -0.1 1,121,785 - aceB HEPCGN_11910 0.50 -0.5 1,121,955 - aceB HEPCGN_11910 0.60 -0.3 1,122,151 - aceB HEPCGN_11910 0.73 -0.3 1,122,539 + +1.1 1,122,540 - +0.9 1,122,548 - -0.1 1,122,668 + -0.0 1,122,818 - -0.4 1,122,884 + +0.1 1,122,892 - -0.8 1,122,907 - -0.2 1,122,912 + -0.3 1,122,912 + -0.7 1,122,913 - +0.4 1,122,913 - +0.1 1,122,915 + -0.2 1,122,916 - +0.2 1,122,932 - -1.1 1,122,999 + metA HEPCGN_11915 0.15 +0.2 1,123,003 + metA HEPCGN_11915 0.16 +1.7 1,123,003 + metA HEPCGN_11915 0.16 -0.2 1,123,005 + metA HEPCGN_11915 0.16 +0.3 1,123,006 - metA HEPCGN_11915 0.16 -0.1 1,123,006 - metA HEPCGN_11915 0.16 -0.5 1,123,129 - metA HEPCGN_11915 0.29 +0.5 1,123,174 - metA HEPCGN_11915 0.34 -0.6 1,123,274 - metA HEPCGN_11915 0.45 -0.5 1,123,336 + metA HEPCGN_11915 0.52 +0.1 1,123,336 + metA HEPCGN_11915 0.52 -0.5 1,123,429 - metA HEPCGN_11915 0.62 -0.8 1,123,475 + metA HEPCGN_11915 0.66 -0.3 1,123,517 - metA HEPCGN_11915 0.71 +0.8
Or see this region's nucleotide sequence