Experiment: Bas39
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and prfH are separated by 56 nucleotides prfH and rtcB overlap by 4 nucleotides rtcB and yafP are separated by 190 nucleotides yafP and dinB overlap by 4 nucleotides
HEPCGN_07805: pepD - cytosol nonspecific dipeptidase, at 303,409 to 304,866
pepD
HEPCGN_07810: prfH - peptide chain release factor H, at 304,923 to 305,537
prfH
HEPCGN_07815: rtcB - RNA ligase RtcB family protein, at 305,534 to 306,673
rtcB
HEPCGN_07820: yafP - GNAT family N-acetyltransferase, at 306,864 to 307,316
yafP
HEPCGN_07825: dinB - DNA polymerase IV, at 307,313 to 308,368
dinB
Position (kb) 305
306
307 Strain fitness (log2 ratio) -2
-1
0
1
2 at 304.617 kb on + strand, within pepD at 304.651 kb on + strand, within pepD at 304.652 kb on - strand, within pepD at 304.950 kb on - strand at 305.160 kb on - strand, within prfH at 305.162 kb on + strand, within prfH at 305.319 kb on - strand, within prfH at 305.526 kb on + strand at 305.646 kb on + strand at 305.646 kb on + strand at 305.736 kb on + strand, within rtcB at 305.758 kb on + strand, within rtcB at 305.833 kb on + strand, within rtcB at 305.848 kb on - strand, within rtcB at 305.848 kb on - strand, within rtcB at 306.005 kb on + strand, within rtcB at 306.031 kb on + strand, within rtcB at 306.070 kb on + strand, within rtcB at 306.072 kb on + strand, within rtcB at 306.073 kb on - strand, within rtcB at 306.073 kb on - strand, within rtcB at 306.078 kb on - strand, within rtcB at 306.402 kb on + strand, within rtcB at 306.548 kb on + strand, within rtcB at 306.549 kb on - strand, within rtcB at 306.584 kb on + strand at 306.585 kb on - strand at 306.585 kb on - strand at 306.634 kb on + strand at 306.634 kb on + strand at 306.635 kb on - strand at 306.635 kb on - strand at 306.635 kb on - strand at 306.661 kb on + strand at 306.663 kb on + strand at 306.664 kb on - strand at 306.664 kb on - strand at 306.687 kb on + strand at 306.792 kb on - strand at 306.830 kb on - strand at 306.888 kb on - strand at 306.935 kb on + strand, within yafP at 306.935 kb on + strand, within yafP at 306.996 kb on - strand, within yafP at 307.027 kb on - strand, within yafP at 307.075 kb on - strand, within yafP at 307.075 kb on - strand, within yafP at 307.085 kb on + strand, within yafP at 307.088 kb on - strand, within yafP at 307.088 kb on - strand, within yafP at 307.103 kb on + strand, within yafP at 307.106 kb on + strand, within yafP at 307.128 kb on - strand, within yafP at 307.219 kb on + strand, within yafP at 307.266 kb on + strand, within yafP at 307.266 kb on + strand, within yafP at 307.266 kb on + strand, within yafP at 307.266 kb on + strand, within yafP at 307.300 kb on - strand at 307.305 kb on + strand at 307.305 kb on + strand at 307.306 kb on - strand at 307.306 kb on - strand at 307.310 kb on + strand at 307.311 kb on - strand at 307.317 kb on - strand at 307.433 kb on + strand, within dinB at 307.434 kb on - strand, within dinB at 307.499 kb on + strand, within dinB at 307.526 kb on + strand, within dinB at 307.527 kb on - strand, within dinB at 307.538 kb on + strand, within dinB at 307.539 kb on - strand, within dinB at 307.569 kb on + strand, within dinB at 307.613 kb on - strand, within dinB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas39 remove 304,617 + pepD HEPCGN_07805 0.83 +0.1 304,651 + pepD HEPCGN_07805 0.85 -0.0 304,652 - pepD HEPCGN_07805 0.85 +0.3 304,950 - +0.2 305,160 - prfH HEPCGN_07810 0.39 -0.1 305,162 + prfH HEPCGN_07810 0.39 -0.3 305,319 - prfH HEPCGN_07810 0.64 -0.4 305,526 + -0.9 305,646 + +0.5 305,646 + -0.4 305,736 + rtcB HEPCGN_07815 0.18 -0.3 305,758 + rtcB HEPCGN_07815 0.20 -0.2 305,833 + rtcB HEPCGN_07815 0.26 +0.5 305,848 - rtcB HEPCGN_07815 0.28 -0.2 305,848 - rtcB HEPCGN_07815 0.28 -0.1 306,005 + rtcB HEPCGN_07815 0.41 +0.4 306,031 + rtcB HEPCGN_07815 0.44 +1.1 306,070 + rtcB HEPCGN_07815 0.47 -0.2 306,072 + rtcB HEPCGN_07815 0.47 +0.4 306,073 - rtcB HEPCGN_07815 0.47 +0.6 306,073 - rtcB HEPCGN_07815 0.47 -0.6 306,078 - rtcB HEPCGN_07815 0.48 +0.4 306,402 + rtcB HEPCGN_07815 0.76 -0.1 306,548 + rtcB HEPCGN_07815 0.89 +0.2 306,549 - rtcB HEPCGN_07815 0.89 -0.4 306,584 + +0.1 306,585 - +0.1 306,585 - +0.1 306,634 + -1.2 306,634 + -1.8 306,635 - -0.6 306,635 - -0.0 306,635 - +0.2 306,661 + +0.1 306,663 + +0.6 306,664 - +0.0 306,664 - -2.1 306,687 + -0.1 306,792 - +0.6 306,830 - +1.8 306,888 - +0.2 306,935 + yafP HEPCGN_07820 0.16 +0.6 306,935 + yafP HEPCGN_07820 0.16 +0.0 306,996 - yafP HEPCGN_07820 0.29 +0.1 307,027 - yafP HEPCGN_07820 0.36 +0.2 307,075 - yafP HEPCGN_07820 0.47 +0.6 307,075 - yafP HEPCGN_07820 0.47 +0.2 307,085 + yafP HEPCGN_07820 0.49 -0.1 307,088 - yafP HEPCGN_07820 0.49 -0.0 307,088 - yafP HEPCGN_07820 0.49 -0.4 307,103 + yafP HEPCGN_07820 0.53 +0.7 307,106 + yafP HEPCGN_07820 0.53 +0.4 307,128 - yafP HEPCGN_07820 0.58 +0.7 307,219 + yafP HEPCGN_07820 0.78 +0.2 307,266 + yafP HEPCGN_07820 0.89 -0.2 307,266 + yafP HEPCGN_07820 0.89 +0.1 307,266 + yafP HEPCGN_07820 0.89 +0.6 307,266 + yafP HEPCGN_07820 0.89 -0.2 307,300 - -0.7 307,305 + -0.6 307,305 + -0.4 307,306 - -0.5 307,306 - +0.3 307,310 + +0.1 307,311 - +0.2 307,317 - -2.1 307,433 + dinB HEPCGN_07825 0.11 -0.2 307,434 - dinB HEPCGN_07825 0.11 -0.2 307,499 + dinB HEPCGN_07825 0.18 -0.5 307,526 + dinB HEPCGN_07825 0.20 -0.7 307,527 - dinB HEPCGN_07825 0.20 +0.6 307,538 + dinB HEPCGN_07825 0.21 +0.7 307,539 - dinB HEPCGN_07825 0.21 +0.4 307,569 + dinB HEPCGN_07825 0.24 +0.6 307,613 - dinB HEPCGN_07825 0.28 -1.0
Or see this region's nucleotide sequence