Strain Fitness in Escherichia coli ECOR38 around HEPCGN_00825

Experiment: Bas39

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcitF and citE are separated by 10 nucleotidescitE and HEPCGN_00825 overlap by 4 nucleotidesHEPCGN_00825 and citC are separated by 14 nucleotides HEPCGN_00815: citF - citrate lyase subunit alpha, at 91,640 to 93,172 citF HEPCGN_00820: citE - citrate (pro-3S)-lyase subunit beta, at 93,183 to 94,091 citE HEPCGN_00825: HEPCGN_00825 - citrate lyase acyl carrier protein 1, at 94,088 to 94,384 _00825 HEPCGN_00830: citC - [citrate (pro-3S)-lyase] ligase, at 94,399 to 95,457 citC Position (kb) 94 95Strain fitness (log2 ratio) -2 -1 0 1at 93.184 kb on + strandat 93.592 kb on + strand, within citEat 93.593 kb on - strand, within citEat 93.593 kb on - strand, within citEat 93.991 kb on + strand, within citEat 94.064 kb on + strandat 94.064 kb on + strandat 94.203 kb on - strand, within HEPCGN_00825at 94.609 kb on - strand, within citCat 94.647 kb on - strand, within citCat 94.697 kb on - strand, within citCat 94.751 kb on + strand, within citCat 94.788 kb on - strand, within citCat 94.817 kb on + strand, within citCat 94.818 kb on - strand, within citCat 94.818 kb on - strand, within citCat 94.867 kb on + strand, within citCat 95.172 kb on - strand, within citCat 95.232 kb on + strand, within citC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas39
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93,184 + -0.0
93,592 + citE HEPCGN_00820 0.45 +0.4
93,593 - citE HEPCGN_00820 0.45 -0.6
93,593 - citE HEPCGN_00820 0.45 +0.3
93,991 + citE HEPCGN_00820 0.89 +0.4
94,064 + -0.3
94,064 + +0.6
94,203 - HEPCGN_00825 0.39 -0.2
94,609 - citC HEPCGN_00830 0.20 -1.2
94,647 - citC HEPCGN_00830 0.23 +0.5
94,697 - citC HEPCGN_00830 0.28 -2.3
94,751 + citC HEPCGN_00830 0.33 -0.0
94,788 - citC HEPCGN_00830 0.37 -0.3
94,817 + citC HEPCGN_00830 0.39 +0.4
94,818 - citC HEPCGN_00830 0.40 +0.1
94,818 - citC HEPCGN_00830 0.40 +0.4
94,867 + citC HEPCGN_00830 0.44 +0.4
95,172 - citC HEPCGN_00830 0.73 +0.9
95,232 + citC HEPCGN_00830 0.79 +0.4

Or see this region's nucleotide sequence