Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS05660 and A4249_RS05665 overlap by 4 nucleotides A4249_RS05665 and A4249_RS05670 are separated by 99 nucleotides A4249_RS05670 and A4249_RS05675 are separated by 80 nucleotides A4249_RS05675 and A4249_RS05680 are separated by 114 nucleotides
A4249_RS05660: A4249_RS05660 - Sec-independent protein translocase protein TatB, at 1,158,148 to 1,158,726
_RS05660
A4249_RS05665: A4249_RS05665 - twin-arginine translocase subunit TatC, at 1,158,723 to 1,159,613
_RS05665
A4249_RS05670: A4249_RS05670 - type I glutamate--ammonia ligase, at 1,159,713 to 1,161,119
_RS05670
A4249_RS05675: A4249_RS05675 - P-II family nitrogen regulator, at 1,161,200 to 1,161,538
_RS05675
A4249_RS05680: A4249_RS05680 - NAD(P)H-hydrate dehydratase, at 1,161,653 to 1,162,483
_RS05680
Position (kb)
1159
1160
1161
1162 Strain fitness (log2 ratio)
-1
0
1
2 at 1159.642 kb on + strand at 1159.642 kb on + strand at 1159.642 kb on + strand at 1159.643 kb on - strand at 1159.643 kb on - strand at 1159.643 kb on - strand at 1159.643 kb on - strand at 1159.698 kb on - strand at 1159.698 kb on - strand at 1161.192 kb on + strand at 1161.193 kb on - strand at 1161.657 kb on + strand at 1161.657 kb on + strand at 1161.657 kb on + strand at 1161.657 kb on + strand at 1161.658 kb on - strand at 1161.658 kb on - strand at 1161.658 kb on - strand at 1161.658 kb on - strand at 1161.912 kb on + strand, within A4249_RS05680 at 1161.912 kb on + strand, within A4249_RS05680 at 1161.912 kb on + strand, within A4249_RS05680 at 1161.913 kb on - strand, within A4249_RS05680 at 1161.913 kb on - strand, within A4249_RS05680 at 1161.913 kb on - strand, within A4249_RS05680 at 1161.913 kb on - strand, within A4249_RS05680 at 1161.966 kb on + strand, within A4249_RS05680 at 1162.071 kb on + strand, within A4249_RS05680 at 1162.071 kb on + strand, within A4249_RS05680 at 1162.071 kb on + strand, within A4249_RS05680 at 1162.071 kb on + strand, within A4249_RS05680 at 1162.071 kb on + strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680 at 1162.072 kb on - strand, within A4249_RS05680
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 1,159,642 + +0.5 1,159,642 + -0.3 1,159,642 + -0.3 1,159,643 - +0.7 1,159,643 - -0.2 1,159,643 - +0.5 1,159,643 - +0.2 1,159,698 - -1.0 1,159,698 - -0.5 1,161,192 + +0.0 1,161,193 - +0.9 1,161,657 + +0.3 1,161,657 + +0.1 1,161,657 + -0.0 1,161,657 + -0.3 1,161,658 - +0.5 1,161,658 - -0.0 1,161,658 - -0.2 1,161,658 - -0.7 1,161,912 + A4249_RS05680 0.31 +0.6 1,161,912 + A4249_RS05680 0.31 +0.3 1,161,912 + A4249_RS05680 0.31 +0.5 1,161,913 - A4249_RS05680 0.31 -0.7 1,161,913 - A4249_RS05680 0.31 -0.0 1,161,913 - A4249_RS05680 0.31 +2.0 1,161,913 - A4249_RS05680 0.31 +0.0 1,161,966 + A4249_RS05680 0.38 +0.2 1,162,071 + A4249_RS05680 0.50 -0.0 1,162,071 + A4249_RS05680 0.50 +0.3 1,162,071 + A4249_RS05680 0.50 -0.4 1,162,071 + A4249_RS05680 0.50 -0.7 1,162,071 + A4249_RS05680 0.50 +0.6 1,162,072 - A4249_RS05680 0.50 +0.7 1,162,072 - A4249_RS05680 0.50 -0.7 1,162,072 - A4249_RS05680 0.50 +0.0 1,162,072 - A4249_RS05680 0.50 +0.5 1,162,072 - A4249_RS05680 0.50 -0.8 1,162,072 - A4249_RS05680 0.50 +1.2 1,162,072 - A4249_RS05680 0.50 -0.8
Or see this region's nucleotide sequence