Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt narG and narK are separated by 392 nucleotides narK and NIAGMN_27705 are separated by 94 nucleotides NIAGMN_27705 and narX are separated by 25 nucleotides
NIAGMN_27695: narG - nitrate reductase subunit alpha, at 5,364,960 to 5,368,703
narG
NIAGMN_27700: narK - nitrate transporter NarK, at 5,369,096 to 5,370,487
narK
NIAGMN_27705: NIAGMN_27705 - hypothetical protein, at 5,370,582 to 5,370,800
_27705
NIAGMN_27710: narX - nitrate/nitrite two-component system sensor histidine kinase NarX, at 5,370,826 to 5,372,622
narX
Position (kb) 5369
5370
5371 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5368.178 kb on - strand, within narG at 5368.290 kb on - strand, within narG at 5368.471 kb on + strand at 5368.923 kb on + strand at 5369.152 kb on - strand at 5370.100 kb on - strand, within narK at 5370.262 kb on + strand, within narK at 5370.263 kb on - strand, within narK at 5370.487 kb on + strand at 5370.488 kb on - strand at 5370.678 kb on - strand, within NIAGMN_27705 at 5370.731 kb on - strand, within NIAGMN_27705 at 5370.731 kb on - strand, within NIAGMN_27705 at 5370.752 kb on + strand, within NIAGMN_27705 at 5370.752 kb on + strand, within NIAGMN_27705 at 5370.823 kb on + strand at 5370.823 kb on + strand at 5370.862 kb on - strand at 5371.480 kb on - strand, within narX
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,368,178 - narG NIAGMN_27695 0.86 +1.4 5,368,290 - narG NIAGMN_27695 0.89 +1.7 5,368,471 + -1.4 5,368,923 + +0.5 5,369,152 - +5.2 5,370,100 - narK NIAGMN_27700 0.72 +0.3 5,370,262 + narK NIAGMN_27700 0.84 +1.1 5,370,263 - narK NIAGMN_27700 0.84 +0.2 5,370,487 + +0.0 5,370,488 - +0.9 5,370,678 - NIAGMN_27705 0.44 +0.0 5,370,731 - NIAGMN_27705 0.68 -2.1 5,370,731 - NIAGMN_27705 0.68 +0.2 5,370,752 + NIAGMN_27705 0.78 -3.1 5,370,752 + NIAGMN_27705 0.78 +0.1 5,370,823 + +0.5 5,370,823 + -0.1 5,370,862 - -2.6 5,371,480 - narX NIAGMN_27710 0.36 +1.1
Or see this region's nucleotide sequence