Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppB and oppA are separated by 85 nucleotides oppA and NIAGMN_27610 are separated by 373 nucleotides NIAGMN_27610 and NIAGMN_27615 overlap by 25 nucleotides NIAGMN_27615 and ychE are separated by 26 nucleotides
NIAGMN_27600: oppB - oligopeptide ABC transporter permease OppB, at 5,347,127 to 5,348,047
oppB
NIAGMN_27605: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 5,348,133 to 5,349,809
oppA
NIAGMN_27610: NIAGMN_27610 - hypothetical protein, at 5,350,183 to 5,350,344
_27610
NIAGMN_27615: NIAGMN_27615 - hypothetical protein, at 5,350,320 to 5,350,475
_27615
NIAGMN_27620: ychE - NAAT family transporter YchE, at 5,350,502 to 5,351,149
ychE
Position (kb) 5348
5349
5350 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5347.160 kb on + strand at 5347.161 kb on - strand at 5347.287 kb on + strand, within oppB at 5347.433 kb on - strand, within oppB at 5347.602 kb on - strand, within oppB at 5347.806 kb on + strand, within oppB at 5348.076 kb on + strand at 5348.125 kb on + strand at 5348.150 kb on - strand at 5348.153 kb on + strand at 5348.164 kb on + strand at 5348.168 kb on + strand at 5348.193 kb on - strand at 5348.234 kb on - strand at 5348.236 kb on + strand at 5348.524 kb on - strand, within oppA at 5348.524 kb on - strand, within oppA at 5348.594 kb on - strand, within oppA at 5348.866 kb on + strand, within oppA at 5348.870 kb on - strand, within oppA at 5348.953 kb on + strand, within oppA at 5349.575 kb on + strand, within oppA at 5349.635 kb on + strand, within oppA at 5349.967 kb on - strand at 5350.288 kb on - strand, within NIAGMN_27610 at 5350.704 kb on + strand, within ychE at 5350.709 kb on - strand, within ychE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,347,160 + -0.4 5,347,161 - +1.9 5,347,287 + oppB NIAGMN_27600 0.17 -0.9 5,347,433 - oppB NIAGMN_27600 0.33 -2.3 5,347,602 - oppB NIAGMN_27600 0.52 +0.9 5,347,806 + oppB NIAGMN_27600 0.74 +0.9 5,348,076 + +0.5 5,348,125 + +1.9 5,348,150 - +0.5 5,348,153 + -0.4 5,348,164 + +0.9 5,348,168 + +0.5 5,348,193 - -1.6 5,348,234 - +1.0 5,348,236 + +0.4 5,348,524 - oppA NIAGMN_27605 0.23 -1.0 5,348,524 - oppA NIAGMN_27605 0.23 -0.5 5,348,594 - oppA NIAGMN_27605 0.27 +0.2 5,348,866 + oppA NIAGMN_27605 0.44 -0.9 5,348,870 - oppA NIAGMN_27605 0.44 +0.4 5,348,953 + oppA NIAGMN_27605 0.49 -1.5 5,349,575 + oppA NIAGMN_27605 0.86 +0.1 5,349,635 + oppA NIAGMN_27605 0.90 +0.3 5,349,967 - +1.5 5,350,288 - NIAGMN_27610 0.65 +0.1 5,350,704 + ychE NIAGMN_27620 0.31 +0.8 5,350,709 - ychE NIAGMN_27620 0.32 -0.3
Or see this region's nucleotide sequence