Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26835

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpCF are separated by 3 nucleotidestrpCF and trpB are separated by 11 nucleotides NIAGMN_26830: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 5,224,107 to 5,225,702 trpD NIAGMN_26835: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 5,225,706 to 5,227,064 trpCF NIAGMN_26840: trpB - tryptophan synthase subunit beta, at 5,227,076 to 5,228,269 trpB Position (kb) 5225 5226 5227 5228Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 5224.970 kb on + strand, within trpDat 5225.272 kb on + strand, within trpDat 5225.590 kb on + strandat 5225.700 kb on + strandat 5225.936 kb on + strand, within trpCFat 5226.477 kb on - strand, within trpCFat 5226.481 kb on + strand, within trpCFat 5226.481 kb on + strand, within trpCFat 5226.624 kb on + strand, within trpCFat 5226.625 kb on - strand, within trpCFat 5226.726 kb on + strand, within trpCFat 5226.831 kb on + strand, within trpCFat 5226.939 kb on - strandat 5226.994 kb on + strandat 5227.087 kb on - strandat 5227.087 kb on - strandat 5227.122 kb on - strandat 5227.472 kb on + strand, within trpBat 5227.911 kb on - strand, within trpBat 5228.045 kb on + strand, within trpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,224,970 + trpD NIAGMN_26830 0.54 -5.3
5,225,272 + trpD NIAGMN_26830 0.73 -0.8
5,225,590 + -1.1
5,225,700 + -3.0
5,225,936 + trpCF NIAGMN_26835 0.17 -4.1
5,226,477 - trpCF NIAGMN_26835 0.57 -2.5
5,226,481 + trpCF NIAGMN_26835 0.57 -1.4
5,226,481 + trpCF NIAGMN_26835 0.57 -1.9
5,226,624 + trpCF NIAGMN_26835 0.68 -4.9
5,226,625 - trpCF NIAGMN_26835 0.68 -5.6
5,226,726 + trpCF NIAGMN_26835 0.75 -3.0
5,226,831 + trpCF NIAGMN_26835 0.83 -4.9
5,226,939 - -0.9
5,226,994 + -1.4
5,227,087 - -2.0
5,227,087 - -2.8
5,227,122 - -4.0
5,227,472 + trpB NIAGMN_26840 0.33 +2.3
5,227,911 - trpB NIAGMN_26840 0.70 -0.4
5,228,045 + trpB NIAGMN_26840 0.81 -0.4

Or see this region's nucleotide sequence