Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26595

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuE and puuB are separated by 37 nucleotidespuuB and puuC are separated by 1 nucleotides NIAGMN_26590: puuE - 4-aminobutyrate transaminase, at 5,171,888 to 5,173,153 puuE NIAGMN_26595: puuB - gamma-glutamylputrescine oxidase, at 5,173,191 to 5,174,471 puuB NIAGMN_26600: puuC - aldehyde dehydrogenase PuuC, at 5,174,473 to 5,175,960 puuC Position (kb) 5173 5174 5175Strain fitness (log2 ratio) -2 -1 0 1at 5172.369 kb on - strand, within puuEat 5172.632 kb on + strand, within puuEat 5172.721 kb on + strand, within puuEat 5173.220 kb on - strandat 5173.868 kb on - strand, within puuB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,172,369 - puuE NIAGMN_26590 0.38 -2.1
5,172,632 + puuE NIAGMN_26590 0.59 -0.1
5,172,721 + puuE NIAGMN_26590 0.66 +1.0
5,173,220 - +0.9
5,173,868 - puuB NIAGMN_26595 0.53 -1.8

Or see this region's nucleotide sequence