Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26450

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjY and NIAGMN_26445 overlap by 1 nucleotidesNIAGMN_26445 and NIAGMN_26450 overlap by 62 nucleotidesNIAGMN_26450 and wcaG are separated by 20 nucleotideswcaG and NIAGMN_26460 are separated by 290 nucleotides NIAGMN_26440: ycjY - Uncharacterized protein YcjY, at 5,145,299 to 5,146,219 ycjY NIAGMN_26445: NIAGMN_26445 - Peptidase-S15 domain-containing protein, at 5,146,219 to 5,146,587 _26445 NIAGMN_26450: NIAGMN_26450 - Hydrolase, at 5,146,526 to 5,147,149 _26450 NIAGMN_26455: wcaG - NAD-dependent dehydratase, at 5,147,170 to 5,147,808 wcaG NIAGMN_26460: NIAGMN_26460 - Murein peptide amidase A, at 5,148,099 to 5,148,827 _26460 Position (kb) 5146 5147 5148Strain fitness (log2 ratio) -2 -1 0 1 2at 5145.848 kb on - strand, within ycjYat 5145.959 kb on - strand, within ycjYat 5145.983 kb on - strand, within ycjYat 5145.986 kb on - strand, within ycjYat 5146.166 kb on + strandat 5146.192 kb on - strandat 5146.228 kb on - strandat 5146.248 kb on + strandat 5146.613 kb on - strand, within NIAGMN_26450at 5146.677 kb on - strand, within NIAGMN_26450at 5146.835 kb on - strand, within NIAGMN_26450at 5146.960 kb on - strand, within NIAGMN_26450at 5147.840 kb on - strandat 5148.020 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,145,848 - ycjY NIAGMN_26440 0.60 +1.6
5,145,959 - ycjY NIAGMN_26440 0.72 -0.4
5,145,983 - ycjY NIAGMN_26440 0.74 -0.9
5,145,986 - ycjY NIAGMN_26440 0.75 -0.2
5,146,166 + +0.3
5,146,192 - -0.8
5,146,228 - +2.7
5,146,248 + +1.5
5,146,613 - NIAGMN_26450 0.14 -2.0
5,146,677 - NIAGMN_26450 0.24 +0.1
5,146,835 - NIAGMN_26450 0.50 -2.5
5,146,960 - NIAGMN_26450 0.70 -2.7
5,147,840 - -1.3
5,148,020 + +1.0

Or see this region's nucleotide sequence