Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26300

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_26295 and recT overlap by 8 nucleotidesrecT and rcbA are separated by 242 nucleotidesrcbA and xisR are separated by 99 nucleotidesxisR and fimB are separated by 1 nucleotides NIAGMN_26295: NIAGMN_26295 - exonuclease VIII, at 5,119,654 to 5,122,254 _26295 NIAGMN_26300: recT - recombination protein RecT, at 5,122,247 to 5,123,056 recT NIAGMN_26305: rcbA - Double-strand break reduction protein, at 5,123,299 to 5,123,487 rcbA NIAGMN_26315: xisR - excisionase XisR, at 5,123,587 to 5,123,802 xisR NIAGMN_26320: fimB - site-specific integrase, at 5,123,804 to 5,125,039 fimB Position (kb) 5122 5123 5124Strain fitness (log2 ratio) -3 -2 -1 0 1at 5121.498 kb on + strand, within NIAGMN_26295at 5121.538 kb on + strand, within NIAGMN_26295at 5121.970 kb on + strand, within NIAGMN_26295at 5122.074 kb on + strandat 5123.804 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,121,498 + NIAGMN_26295 0.71 +0.8
5,121,538 + NIAGMN_26295 0.72 -3.1
5,121,970 + NIAGMN_26295 0.89 +0.3
5,122,074 + +1.0
5,123,804 - -3.0

Or see this region's nucleotide sequence