Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcK and rimL are separated by 234 nucleotides rimL and ydcH are separated by 62 nucleotides ydcH and opgD are separated by 139 nucleotides opgD and ydcJ are separated by 260 nucleotides
NIAGMN_25765: ydcK - YdcK family protein, at 5,027,943 to 5,028,923
ydcK
NIAGMN_25770: rimL - 50S ribosomal protein L7/L12-serine acetyltransferase, at 5,029,158 to 5,029,454
rimL
NIAGMN_25775: ydcH - Uncharacterized protein YdcH, at 5,029,517 to 5,029,741
ydcH
NIAGMN_25780: opgD - glucan biosynthesis protein OpgD, at 5,029,881 to 5,031,500
opgD
NIAGMN_25785: ydcJ - Uncharacterized protein YdcJ, at 5,031,761 to 5,033,104
ydcJ
Position (kb) 5029
5030
5031
5032 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5028.920 kb on - strand at 5029.519 kb on - strand at 5029.519 kb on - strand at 5029.782 kb on - strand at 5029.782 kb on - strand at 5029.907 kb on - strand at 5030.069 kb on - strand, within opgD at 5030.471 kb on - strand, within opgD at 5030.492 kb on - strand, within opgD at 5030.746 kb on + strand, within opgD at 5030.769 kb on - strand, within opgD at 5030.769 kb on - strand, within opgD at 5031.013 kb on - strand, within opgD at 5031.119 kb on - strand, within opgD at 5031.294 kb on - strand, within opgD at 5031.952 kb on - strand, within ydcJ at 5032.189 kb on - strand, within ydcJ at 5032.421 kb on - strand, within ydcJ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,028,920 - +1.1 5,029,519 - +0.2 5,029,519 - -1.5 5,029,782 - -2.2 5,029,782 - +0.3 5,029,907 - +1.9 5,030,069 - opgD NIAGMN_25780 0.12 +1.1 5,030,471 - opgD NIAGMN_25780 0.36 -1.4 5,030,492 - opgD NIAGMN_25780 0.38 -0.4 5,030,746 + opgD NIAGMN_25780 0.53 -0.3 5,030,769 - opgD NIAGMN_25780 0.55 -0.2 5,030,769 - opgD NIAGMN_25780 0.55 +1.0 5,031,013 - opgD NIAGMN_25780 0.70 -2.6 5,031,119 - opgD NIAGMN_25780 0.76 -1.6 5,031,294 - opgD NIAGMN_25780 0.87 +1.3 5,031,952 - ydcJ NIAGMN_25785 0.14 -0.7 5,032,189 - ydcJ NIAGMN_25785 0.32 +0.5 5,032,421 - ydcJ NIAGMN_25785 0.49 +0.6
Or see this region's nucleotide sequence