Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hicB and hicA are separated by 24 nucleotides hicA and yncJ are separated by 281 nucleotides yncJ and rlhA are separated by 91 nucleotides rlhA and sutR are separated by 30 nucleotides sutR and ydcO are separated by 91 nucleotides
NIAGMN_25720: hicB - type II toxin-antitoxin system antitoxin HicB, at 5,020,214 to 5,020,651
hicB
NIAGMN_25725: hicA - type II toxin-antitoxin system mRNA interferase toxin HicA, at 5,020,676 to 5,020,852
hicA
NIAGMN_25730: yncJ - Uncharacterized protein YncJ, at 5,021,134 to 5,021,304
yncJ
NIAGMN_25735: rlhA - 23S rRNA 5-hydroxycytidine C2501 synthase, at 5,021,396 to 5,023,399
rlhA
NIAGMN_25740: sutR - DNA-binding transcriptional regulator SutR, at 5,023,430 to 5,023,966
sutR
NIAGMN_25745: ydcO - BenE family transporter YdcO, at 5,024,058 to 5,025,230
ydcO
Position (kb)
5021
5022
5023
5024 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5020.416 kb on - strand, within hicB at 5020.438 kb on + strand, within hicB at 5020.875 kb on + strand at 5020.876 kb on - strand at 5020.934 kb on - strand at 5021.016 kb on - strand at 5021.043 kb on + strand at 5021.351 kb on - strand at 5021.547 kb on - strand at 5021.694 kb on + strand, within rlhA at 5021.835 kb on + strand, within rlhA at 5021.930 kb on - strand, within rlhA at 5021.930 kb on - strand, within rlhA at 5022.598 kb on + strand, within rlhA at 5023.868 kb on - strand, within sutR at 5023.923 kb on - strand at 5023.923 kb on - strand at 5024.058 kb on - strand at 5024.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,020,416 - hicB NIAGMN_25720 0.46 -0.5 5,020,438 + hicB NIAGMN_25720 0.51 -1.5 5,020,875 + -1.6 5,020,876 - -1.2 5,020,934 - +1.9 5,021,016 - -2.8 5,021,043 + +0.0 5,021,351 - -1.2 5,021,547 - -3.4 5,021,694 + rlhA NIAGMN_25735 0.15 -2.1 5,021,835 + rlhA NIAGMN_25735 0.22 -1.1 5,021,930 - rlhA NIAGMN_25735 0.27 +1.5 5,021,930 - rlhA NIAGMN_25735 0.27 -0.5 5,022,598 + rlhA NIAGMN_25735 0.60 -1.5 5,023,868 - sutR NIAGMN_25740 0.82 -1.7 5,023,923 - +1.3 5,023,923 - -1.1 5,024,058 - -0.5 5,024,114 - +0.1
Or see this region's nucleotide sequence