Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25450

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdosP and ddpX are separated by 257 nucleotidesddpX and ddpA are separated by 13 nucleotidesddpA and ddpB are separated by 42 nucleotides NIAGMN_25440: dosP - oxygen-sensing cyclic-di-GMP phosphodiesterase, at 4,960,174 to 4,961,325 dosP NIAGMN_25445: ddpX - D-alanyl-D-alanine dipeptidase, at 4,961,583 to 4,962,164 ddpX NIAGMN_25450: ddpA - putative D,D-dipeptide-binding periplasmic protein DdpA, at 4,962,178 to 4,963,728 ddpA NIAGMN_25455: ddpB - putative D,D-dipeptide transport system permease protein DdpB, at 4,963,771 to 4,964,751 ddpB Position (kb) 4962 4963 4964Strain fitness (log2 ratio) -3 -2 -1 0 1at 4961.493 kb on + strandat 4961.664 kb on - strand, within ddpXat 4962.616 kb on - strand, within ddpAat 4962.991 kb on + strand, within ddpAat 4963.044 kb on + strand, within ddpAat 4963.441 kb on - strand, within ddpAat 4963.714 kb on - strandat 4963.726 kb on + strandat 4963.750 kb on - strandat 4963.807 kb on - strandat 4963.919 kb on - strand, within ddpBat 4964.278 kb on + strand, within ddpBat 4964.472 kb on + strand, within ddpBat 4964.480 kb on + strand, within ddpBat 4964.552 kb on - strand, within ddpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,961,493 + -3.0
4,961,664 - ddpX NIAGMN_25445 0.14 -0.3
4,962,616 - ddpA NIAGMN_25450 0.28 -0.5
4,962,991 + ddpA NIAGMN_25450 0.52 +0.6
4,963,044 + ddpA NIAGMN_25450 0.56 +0.3
4,963,441 - ddpA NIAGMN_25450 0.81 +0.3
4,963,714 - +1.3
4,963,726 + -0.3
4,963,750 - +0.4
4,963,807 - +0.5
4,963,919 - ddpB NIAGMN_25455 0.15 -1.3
4,964,278 + ddpB NIAGMN_25455 0.52 +1.2
4,964,472 + ddpB NIAGMN_25455 0.71 -2.1
4,964,480 + ddpB NIAGMN_25455 0.72 -1.0
4,964,552 - ddpB NIAGMN_25455 0.80 -0.2

Or see this region's nucleotide sequence