Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yneJ and sad are separated by 100 nucleotides sad and glsB are separated by 63 nucleotides glsB and yneG overlap by 1 nucleotides
NIAGMN_25250: yneJ - Uncharacterized HTH-type transcriptional regulator YneJ, at 4,905,806 to 4,906,687
yneJ
NIAGMN_25255: sad - succinate-semialdehyde dehydrogenase, at 4,906,788 to 4,908,176
sad
NIAGMN_25260: glsB - glutaminase B, at 4,908,240 to 4,909,166
glsB
NIAGMN_25265: yneG - Uncharacterized protein YneG, at 4,909,166 to 4,909,525
yneG
Position (kb)
4906
4907
4908
4909 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4905.977 kb on - strand, within yneJ at 4906.237 kb on + strand, within yneJ at 4906.489 kb on - strand, within yneJ at 4906.518 kb on - strand, within yneJ at 4906.553 kb on + strand, within yneJ at 4906.708 kb on - strand at 4906.796 kb on + strand at 4906.936 kb on + strand, within sad at 4907.085 kb on + strand, within sad at 4907.086 kb on - strand, within sad at 4907.123 kb on + strand, within sad at 4907.123 kb on + strand, within sad at 4907.186 kb on + strand, within sad at 4907.257 kb on - strand, within sad at 4907.260 kb on + strand, within sad at 4907.264 kb on + strand, within sad at 4907.341 kb on + strand, within sad at 4907.342 kb on - strand, within sad at 4907.342 kb on - strand, within sad at 4907.823 kb on + strand, within sad at 4907.951 kb on + strand, within sad at 4908.197 kb on + strand at 4908.587 kb on + strand, within glsB at 4908.965 kb on + strand, within glsB at 4908.965 kb on + strand, within glsB at 4908.969 kb on + strand, within glsB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,905,977 - yneJ NIAGMN_25250 0.19 +1.1 4,906,237 + yneJ NIAGMN_25250 0.49 +1.7 4,906,489 - yneJ NIAGMN_25250 0.77 -0.2 4,906,518 - yneJ NIAGMN_25250 0.81 -0.0 4,906,553 + yneJ NIAGMN_25250 0.85 +0.2 4,906,708 - -0.8 4,906,796 + +0.6 4,906,936 + sad NIAGMN_25255 0.11 -0.5 4,907,085 + sad NIAGMN_25255 0.21 -0.5 4,907,086 - sad NIAGMN_25255 0.21 +0.1 4,907,123 + sad NIAGMN_25255 0.24 -0.1 4,907,123 + sad NIAGMN_25255 0.24 +0.1 4,907,186 + sad NIAGMN_25255 0.29 +1.5 4,907,257 - sad NIAGMN_25255 0.34 -0.1 4,907,260 + sad NIAGMN_25255 0.34 -2.8 4,907,264 + sad NIAGMN_25255 0.34 -0.1 4,907,341 + sad NIAGMN_25255 0.40 +1.1 4,907,342 - sad NIAGMN_25255 0.40 -0.8 4,907,342 - sad NIAGMN_25255 0.40 +0.2 4,907,823 + sad NIAGMN_25255 0.75 -0.8 4,907,951 + sad NIAGMN_25255 0.84 -1.1 4,908,197 + -1.3 4,908,587 + glsB NIAGMN_25260 0.37 -0.9 4,908,965 + glsB NIAGMN_25260 0.78 +0.8 4,908,965 + glsB NIAGMN_25260 0.78 -0.7 4,908,969 + glsB NIAGMN_25260 0.79 +0.7
Or see this region's nucleotide sequence