Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24315

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthinT and NIAGMN_24310 are separated by 2 nucleotidesNIAGMN_24310 and lpoB are separated by 13 nucleotideslpoB and thiK overlap by 20 nucleotidesthiK and nagZ are separated by 10 nucleotides NIAGMN_24305: hinT - purine nucleoside phosphoramidase, at 4,754,740 to 4,755,099 hinT NIAGMN_24310: NIAGMN_24310 - DUF1425 domain-containing protein, at 4,755,102 to 4,755,479 _24310 NIAGMN_24315: lpoB - penicillin-binding protein activator LpoB, at 4,755,493 to 4,756,134 lpoB NIAGMN_24320: thiK - thiamine kinase, at 4,756,115 to 4,756,939 thiK NIAGMN_24325: nagZ - beta-N-acetylhexosaminidase, at 4,756,950 to 4,757,975 nagZ Position (kb) 4755 4756 4757Strain fitness (log2 ratio) -2 -1 0 1at 4754.596 kb on - strandat 4754.600 kb on - strandat 4754.640 kb on - strandat 4754.670 kb on + strandat 4754.675 kb on + strandat 4755.760 kb on - strand, within lpoBat 4756.199 kb on - strand, within thiKat 4756.318 kb on - strand, within thiKat 4756.356 kb on + strand, within thiKat 4756.356 kb on + strand, within thiKat 4756.356 kb on + strand, within thiKat 4756.385 kb on + strand, within thiKat 4756.508 kb on + strand, within thiKat 4756.797 kb on + strand, within thiKat 4756.929 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,754,596 - +1.7
4,754,600 - -2.0
4,754,640 - -0.2
4,754,670 + +0.1
4,754,675 + -0.3
4,755,760 - lpoB NIAGMN_24315 0.42 +0.2
4,756,199 - thiK NIAGMN_24320 0.10 +0.7
4,756,318 - thiK NIAGMN_24320 0.25 -1.0
4,756,356 + thiK NIAGMN_24320 0.29 +0.7
4,756,356 + thiK NIAGMN_24320 0.29 +1.6
4,756,356 + thiK NIAGMN_24320 0.29 +0.1
4,756,385 + thiK NIAGMN_24320 0.33 +1.1
4,756,508 + thiK NIAGMN_24320 0.48 -2.3
4,756,797 + thiK NIAGMN_24320 0.83 +0.4
4,756,929 + -1.3

Or see this region's nucleotide sequence