Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaB and hyaC are separated by 18 nucleotides hyaC and hyaD overlap by 4 nucleotides hyaD and NIAGMN_23060 overlap by 4 nucleotides NIAGMN_23060 and NIAGMN_23065 overlap by 4 nucleotides
NIAGMN_23045: hyaB - Ni/Fe-hydrogenase large subunit, at 4,533,679 to 4,535,472
hyaB
NIAGMN_23050: hyaC - Ni/Fe-hydrogenase b-type cytochrome subunit, at 4,535,491 to 4,536,198
hyaC
NIAGMN_23055: hyaD - hydrogenase 1 maturation protease, at 4,536,195 to 4,536,782
hyaD
NIAGMN_23060: NIAGMN_23060 - hydrogenase 1 maturation protease, at 4,536,779 to 4,537,177
_23060
NIAGMN_23065: NIAGMN_23065 - hydrogenase expression/formation protein, at 4,537,174 to 4,538,031
_23065
Position (kb) 4535
4536
4537 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4534.798 kb on + strand, within hyaB at 4534.861 kb on + strand, within hyaB at 4534.862 kb on - strand, within hyaB at 4535.070 kb on + strand, within hyaB at 4535.547 kb on + strand at 4535.548 kb on - strand at 4535.570 kb on + strand, within hyaC at 4535.570 kb on + strand, within hyaC at 4535.570 kb on + strand, within hyaC at 4535.645 kb on - strand, within hyaC at 4535.653 kb on + strand, within hyaC at 4535.654 kb on - strand, within hyaC at 4535.654 kb on - strand, within hyaC at 4535.654 kb on - strand, within hyaC at 4535.824 kb on + strand, within hyaC at 4535.858 kb on + strand, within hyaC at 4535.916 kb on + strand, within hyaC at 4535.941 kb on + strand, within hyaC at 4535.959 kb on + strand, within hyaC at 4536.088 kb on + strand, within hyaC at 4536.088 kb on + strand, within hyaC at 4536.088 kb on + strand, within hyaC at 4536.088 kb on + strand, within hyaC at 4536.090 kb on + strand, within hyaC at 4536.176 kb on + strand at 4536.217 kb on + strand at 4536.217 kb on + strand at 4536.282 kb on + strand, within hyaD at 4536.430 kb on - strand, within hyaD at 4536.454 kb on - strand, within hyaD at 4536.684 kb on - strand, within hyaD at 4536.705 kb on + strand, within hyaD at 4536.705 kb on + strand, within hyaD at 4536.980 kb on + strand, within NIAGMN_23060
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,534,798 + hyaB NIAGMN_23045 0.62 +1.5 4,534,861 + hyaB NIAGMN_23045 0.66 +0.9 4,534,862 - hyaB NIAGMN_23045 0.66 -1.4 4,535,070 + hyaB NIAGMN_23045 0.78 +1.6 4,535,547 + -0.8 4,535,548 - +0.2 4,535,570 + hyaC NIAGMN_23050 0.11 +3.2 4,535,570 + hyaC NIAGMN_23050 0.11 -1.0 4,535,570 + hyaC NIAGMN_23050 0.11 -0.2 4,535,645 - hyaC NIAGMN_23050 0.22 -2.1 4,535,653 + hyaC NIAGMN_23050 0.23 -0.2 4,535,654 - hyaC NIAGMN_23050 0.23 +1.0 4,535,654 - hyaC NIAGMN_23050 0.23 +0.7 4,535,654 - hyaC NIAGMN_23050 0.23 -0.5 4,535,824 + hyaC NIAGMN_23050 0.47 -1.9 4,535,858 + hyaC NIAGMN_23050 0.52 -0.4 4,535,916 + hyaC NIAGMN_23050 0.60 -1.0 4,535,941 + hyaC NIAGMN_23050 0.64 -0.9 4,535,959 + hyaC NIAGMN_23050 0.66 -1.7 4,536,088 + hyaC NIAGMN_23050 0.84 -0.1 4,536,088 + hyaC NIAGMN_23050 0.84 -0.6 4,536,088 + hyaC NIAGMN_23050 0.84 -3.4 4,536,088 + hyaC NIAGMN_23050 0.84 +2.0 4,536,090 + hyaC NIAGMN_23050 0.85 -2.1 4,536,176 + -0.0 4,536,217 + -1.9 4,536,217 + -1.1 4,536,282 + hyaD NIAGMN_23055 0.15 -0.1 4,536,430 - hyaD NIAGMN_23055 0.40 -1.6 4,536,454 - hyaD NIAGMN_23055 0.44 -1.2 4,536,684 - hyaD NIAGMN_23055 0.83 -1.5 4,536,705 + hyaD NIAGMN_23055 0.87 -0.3 4,536,705 + hyaD NIAGMN_23055 0.87 -1.1 4,536,980 + NIAGMN_23060 0.50 -0.8
Or see this region's nucleotide sequence