Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22370

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotideswzx and rfbE are separated by 2 nucleotides NIAGMN_22360: wzy - O157 family O-antigen polymerase, at 4,413,877 to 4,415,061 wzy NIAGMN_22365: wcaA - glycosyl transferase, at 4,415,030 to 4,415,776 wcaA NIAGMN_22370: wzx - O157 family O-antigen flippase, at 4,415,763 to 4,417,154 wzx NIAGMN_22375: rfbE - GDP-perosamine synthase RfbE/PerA, at 4,417,157 to 4,418,251 rfbE Position (kb) 4415 4416 4417 4418Strain fitness (log2 ratio) -1 0 1at 4414.825 kb on + strand, within wzyat 4418.040 kb on + strand, within rfbE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,414,825 + wzy NIAGMN_22360 0.80 +1.2
4,418,040 + rfbE NIAGMN_22375 0.81 -1.0

Or see this region's nucleotide sequence