Strain Fitness in Escherichia coli ECRC102 around NIAGMN_21300

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmglB and mglA are separated by 60 nucleotidesmglA and mglC are separated by 15 nucleotides NIAGMN_21295: mglB - galactose/glucose ABC transporter substrate-binding protein MglB, at 4,225,016 to 4,226,014 mglB NIAGMN_21300: mglA - galactose/methyl galactoside ABC transporter ATP-binding protein MglA, at 4,226,075 to 4,227,595 mglA NIAGMN_21305: mglC - galactose/methyl galactoside ABC transporter permease MglC, at 4,227,611 to 4,228,621 mglC Position (kb) 4226 4227 4228Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4225.112 kb on + strandat 4225.245 kb on + strand, within mglBat 4225.245 kb on + strand, within mglBat 4225.354 kb on + strand, within mglBat 4225.388 kb on + strand, within mglBat 4225.403 kb on + strand, within mglBat 4225.515 kb on + strand, within mglBat 4225.565 kb on + strand, within mglBat 4225.565 kb on + strand, within mglBat 4225.565 kb on + strand, within mglBat 4225.654 kb on + strand, within mglBat 4225.858 kb on + strand, within mglBat 4225.859 kb on - strand, within mglBat 4225.967 kb on + strandat 4225.977 kb on + strandat 4225.978 kb on - strandat 4226.066 kb on + strandat 4226.686 kb on + strand, within mglAat 4226.686 kb on + strand, within mglAat 4226.718 kb on + strand, within mglAat 4226.881 kb on + strand, within mglAat 4226.881 kb on + strand, within mglAat 4226.881 kb on + strand, within mglAat 4228.163 kb on + strand, within mglCat 4228.163 kb on + strand, within mglCat 4228.164 kb on - strand, within mglCat 4228.175 kb on + strand, within mglCat 4228.175 kb on + strand, within mglCat 4228.313 kb on + strand, within mglCat 4228.550 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,225,112 + -0.9
4,225,245 + mglB NIAGMN_21295 0.23 -2.0
4,225,245 + mglB NIAGMN_21295 0.23 -0.7
4,225,354 + mglB NIAGMN_21295 0.34 -2.4
4,225,388 + mglB NIAGMN_21295 0.37 +1.4
4,225,403 + mglB NIAGMN_21295 0.39 +1.6
4,225,515 + mglB NIAGMN_21295 0.50 +1.3
4,225,565 + mglB NIAGMN_21295 0.55 +1.1
4,225,565 + mglB NIAGMN_21295 0.55 -1.1
4,225,565 + mglB NIAGMN_21295 0.55 +0.7
4,225,654 + mglB NIAGMN_21295 0.64 -2.1
4,225,858 + mglB NIAGMN_21295 0.84 +1.8
4,225,859 - mglB NIAGMN_21295 0.84 -1.1
4,225,967 + +0.5
4,225,977 + -0.4
4,225,978 - +1.6
4,226,066 + -1.0
4,226,686 + mglA NIAGMN_21300 0.40 +1.6
4,226,686 + mglA NIAGMN_21300 0.40 -0.1
4,226,718 + mglA NIAGMN_21300 0.42 -1.8
4,226,881 + mglA NIAGMN_21300 0.53 +1.1
4,226,881 + mglA NIAGMN_21300 0.53 +1.3
4,226,881 + mglA NIAGMN_21300 0.53 -2.5
4,228,163 + mglC NIAGMN_21305 0.55 -0.6
4,228,163 + mglC NIAGMN_21305 0.55 -1.6
4,228,164 - mglC NIAGMN_21305 0.55 +1.5
4,228,175 + mglC NIAGMN_21305 0.56 +1.3
4,228,175 + mglC NIAGMN_21305 0.56 -2.6
4,228,313 + mglC NIAGMN_21305 0.69 -0.7
4,228,550 + +2.8

Or see this region's nucleotide sequence