Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_20375 and smrB are separated by 170 nucleotides smrB and prmB are separated by 165 nucleotides prmB and aroC are separated by 34 nucleotides
NIAGMN_20375: NIAGMN_20375 - DUF2544 domain-containing protein, at 4,025,139 to 4,025,981
_20375
NIAGMN_20380: smrB - endonuclease SmrB, at 4,026,152 to 4,026,703
smrB
NIAGMN_20385: prmB - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,026,869 to 4,027,801
prmB
NIAGMN_20390: aroC - chorismate synthase, at 4,027,836 to 4,028,921
aroC
Position (kb)
4026
4027
4028 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4025.893 kb on + strand, within NIAGMN_20375 at 4025.918 kb on + strand at 4025.961 kb on + strand at 4025.961 kb on + strand at 4025.961 kb on + strand at 4025.962 kb on - strand at 4025.962 kb on - strand at 4025.962 kb on - strand at 4025.962 kb on - strand at 4025.962 kb on - strand at 4025.966 kb on - strand at 4025.979 kb on + strand at 4025.980 kb on - strand at 4025.980 kb on - strand at 4026.019 kb on - strand at 4026.027 kb on + strand at 4026.027 kb on + strand at 4026.147 kb on - strand at 4026.150 kb on - strand at 4026.150 kb on - strand at 4026.220 kb on - strand, within smrB at 4026.220 kb on - strand, within smrB at 4026.293 kb on + strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.294 kb on - strand, within smrB at 4026.295 kb on + strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.296 kb on - strand, within smrB at 4026.480 kb on - strand, within smrB at 4026.480 kb on - strand, within smrB at 4026.483 kb on + strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.484 kb on - strand, within smrB at 4026.506 kb on + strand, within smrB at 4026.629 kb on - strand, within smrB at 4026.683 kb on - strand at 4026.706 kb on - strand at 4026.706 kb on - strand at 4026.706 kb on - strand at 4026.716 kb on - strand at 4026.716 kb on - strand at 4026.807 kb on + strand at 4026.829 kb on + strand at 4026.867 kb on - strand at 4026.867 kb on - strand at 4026.897 kb on + strand at 4026.898 kb on - strand at 4026.898 kb on - strand at 4026.962 kb on - strand at 4027.054 kb on + strand, within prmB at 4027.142 kb on + strand, within prmB at 4027.143 kb on - strand, within prmB at 4027.181 kb on + strand, within prmB at 4027.278 kb on + strand, within prmB at 4027.278 kb on + strand, within prmB at 4027.278 kb on + strand, within prmB at 4027.278 kb on + strand, within prmB at 4027.279 kb on - strand, within prmB at 4027.282 kb on + strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.283 kb on - strand, within prmB at 4027.322 kb on + strand, within prmB at 4027.323 kb on - strand, within prmB at 4027.353 kb on + strand, within prmB at 4027.357 kb on + strand, within prmB at 4027.358 kb on - strand, within prmB at 4027.513 kb on + strand, within prmB at 4027.638 kb on + strand, within prmB at 4027.668 kb on + strand, within prmB at 4027.668 kb on + strand, within prmB at 4027.668 kb on + strand, within prmB at 4027.668 kb on + strand, within prmB at 4027.675 kb on + strand, within prmB at 4027.675 kb on + strand, within prmB at 4027.741 kb on + strand at 4027.790 kb on + strand at 4027.799 kb on + strand at 4027.799 kb on + strand at 4027.799 kb on + strand at 4027.803 kb on + strand at 4028.000 kb on + strand, within aroC at 4028.001 kb on - strand, within aroC at 4028.118 kb on + strand, within aroC at 4028.118 kb on + strand, within aroC at 4028.118 kb on + strand, within aroC at 4028.119 kb on - strand, within aroC at 4028.119 kb on - strand, within aroC at 4028.160 kb on + strand, within aroC at 4028.166 kb on + strand, within aroC at 4028.401 kb on + strand, within aroC at 4028.645 kb on + strand, within aroC at 4028.675 kb on + strand, within aroC at 4028.675 kb on + strand, within aroC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,025,893 + NIAGMN_20375 0.89 +1.2 4,025,918 + -2.5 4,025,961 + -1.3 4,025,961 + +0.2 4,025,961 + -1.3 4,025,962 - -1.9 4,025,962 - -1.1 4,025,962 - +1.4 4,025,962 - +1.5 4,025,962 - +0.5 4,025,966 - +0.2 4,025,979 + -0.3 4,025,980 - +1.7 4,025,980 - -0.3 4,026,019 - +1.1 4,026,027 + +0.1 4,026,027 + -0.3 4,026,147 - -0.7 4,026,150 - +0.9 4,026,150 - -1.0 4,026,220 - smrB NIAGMN_20380 0.12 -1.3 4,026,220 - smrB NIAGMN_20380 0.12 +0.8 4,026,293 + smrB NIAGMN_20380 0.26 +0.4 4,026,294 - smrB NIAGMN_20380 0.26 +0.9 4,026,294 - smrB NIAGMN_20380 0.26 -1.1 4,026,294 - smrB NIAGMN_20380 0.26 -0.2 4,026,294 - smrB NIAGMN_20380 0.26 +1.2 4,026,294 - smrB NIAGMN_20380 0.26 +2.1 4,026,294 - smrB NIAGMN_20380 0.26 +1.8 4,026,295 + smrB NIAGMN_20380 0.26 +2.2 4,026,296 - smrB NIAGMN_20380 0.26 -1.5 4,026,296 - smrB NIAGMN_20380 0.26 -3.8 4,026,296 - smrB NIAGMN_20380 0.26 -0.2 4,026,296 - smrB NIAGMN_20380 0.26 +0.3 4,026,296 - smrB NIAGMN_20380 0.26 -0.3 4,026,296 - smrB NIAGMN_20380 0.26 +2.4 4,026,480 - smrB NIAGMN_20380 0.59 -1.1 4,026,480 - smrB NIAGMN_20380 0.59 +0.7 4,026,483 + smrB NIAGMN_20380 0.60 +0.6 4,026,484 - smrB NIAGMN_20380 0.60 -1.1 4,026,484 - smrB NIAGMN_20380 0.60 -2.2 4,026,484 - smrB NIAGMN_20380 0.60 +0.1 4,026,484 - smrB NIAGMN_20380 0.60 -1.7 4,026,484 - smrB NIAGMN_20380 0.60 -0.2 4,026,484 - smrB NIAGMN_20380 0.60 +0.2 4,026,484 - smrB NIAGMN_20380 0.60 +0.2 4,026,484 - smrB NIAGMN_20380 0.60 +2.1 4,026,484 - smrB NIAGMN_20380 0.60 +1.4 4,026,484 - smrB NIAGMN_20380 0.60 +0.6 4,026,506 + smrB NIAGMN_20380 0.64 -1.9 4,026,629 - smrB NIAGMN_20380 0.86 -0.4 4,026,683 - +1.7 4,026,706 - -0.9 4,026,706 - +0.5 4,026,706 - -1.0 4,026,716 - +1.4 4,026,716 - +0.1 4,026,807 + +1.7 4,026,829 + +0.0 4,026,867 - +0.1 4,026,867 - +0.5 4,026,897 + -1.5 4,026,898 - -0.3 4,026,898 - +1.0 4,026,962 - -0.4 4,027,054 + prmB NIAGMN_20385 0.20 +0.3 4,027,142 + prmB NIAGMN_20385 0.29 +0.3 4,027,143 - prmB NIAGMN_20385 0.29 -0.3 4,027,181 + prmB NIAGMN_20385 0.33 +0.7 4,027,278 + prmB NIAGMN_20385 0.44 -1.6 4,027,278 + prmB NIAGMN_20385 0.44 +0.7 4,027,278 + prmB NIAGMN_20385 0.44 -1.5 4,027,278 + prmB NIAGMN_20385 0.44 -2.5 4,027,279 - prmB NIAGMN_20385 0.44 +0.9 4,027,282 + prmB NIAGMN_20385 0.44 +0.7 4,027,283 - prmB NIAGMN_20385 0.44 -0.3 4,027,283 - prmB NIAGMN_20385 0.44 +2.0 4,027,283 - prmB NIAGMN_20385 0.44 -1.6 4,027,283 - prmB NIAGMN_20385 0.44 +0.1 4,027,283 - prmB NIAGMN_20385 0.44 +1.0 4,027,283 - prmB NIAGMN_20385 0.44 -1.4 4,027,283 - prmB NIAGMN_20385 0.44 -0.6 4,027,283 - prmB NIAGMN_20385 0.44 -2.1 4,027,322 + prmB NIAGMN_20385 0.49 -0.6 4,027,323 - prmB NIAGMN_20385 0.49 -1.8 4,027,353 + prmB NIAGMN_20385 0.52 -2.8 4,027,357 + prmB NIAGMN_20385 0.52 +0.0 4,027,358 - prmB NIAGMN_20385 0.52 -1.1 4,027,513 + prmB NIAGMN_20385 0.69 -0.1 4,027,638 + prmB NIAGMN_20385 0.82 +1.5 4,027,668 + prmB NIAGMN_20385 0.86 +0.4 4,027,668 + prmB NIAGMN_20385 0.86 -1.0 4,027,668 + prmB NIAGMN_20385 0.86 -1.1 4,027,668 + prmB NIAGMN_20385 0.86 -0.9 4,027,675 + prmB NIAGMN_20385 0.86 +1.0 4,027,675 + prmB NIAGMN_20385 0.86 +1.5 4,027,741 + -2.5 4,027,790 + -0.3 4,027,799 + +0.8 4,027,799 + -1.0 4,027,799 + -1.6 4,027,803 + -1.2 4,028,000 + aroC NIAGMN_20390 0.15 +1.2 4,028,001 - aroC NIAGMN_20390 0.15 -1.1 4,028,118 + aroC NIAGMN_20390 0.26 -2.0 4,028,118 + aroC NIAGMN_20390 0.26 +0.6 4,028,118 + aroC NIAGMN_20390 0.26 -1.5 4,028,119 - aroC NIAGMN_20390 0.26 -2.3 4,028,119 - aroC NIAGMN_20390 0.26 -3.0 4,028,160 + aroC NIAGMN_20390 0.30 -0.3 4,028,166 + aroC NIAGMN_20390 0.30 -2.9 4,028,401 + aroC NIAGMN_20390 0.52 +1.8 4,028,645 + aroC NIAGMN_20390 0.74 -0.2 4,028,675 + aroC NIAGMN_20390 0.77 +0.9 4,028,675 + aroC NIAGMN_20390 0.77 -1.0
Or see this region's nucleotide sequence