Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20220

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntemrK and emrY overlap by 1 nucleotidesemrY and dsdA are separated by 107 nucleotides NIAGMN_20215: emrK - multidrug efflux MFS transporter periplasmic adaptor subunit EmrK, at 3,990,196 to 3,991,359 emrK NIAGMN_20220: emrY - multidrug efflux MFS transporter permease subunit EmrY, at 3,991,359 to 3,992,897 emrY NIAGMN_20225: dsdA - D-serine ammonia-lyase, at 3,993,005 to 3,994,333 dsdA Position (kb) 3991 3992 3993Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3990.547 kb on + strand, within emrKat 3990.602 kb on - strand, within emrKat 3990.677 kb on + strand, within emrKat 3990.828 kb on + strand, within emrKat 3990.828 kb on + strand, within emrKat 3990.847 kb on + strand, within emrKat 3990.848 kb on - strand, within emrKat 3990.971 kb on + strand, within emrKat 3990.971 kb on + strand, within emrKat 3990.996 kb on + strand, within emrKat 3991.079 kb on + strand, within emrKat 3991.207 kb on - strand, within emrKat 3992.189 kb on + strand, within emrYat 3992.251 kb on + strand, within emrYat 3992.273 kb on + strand, within emrYat 3992.416 kb on - strand, within emrYat 3992.435 kb on + strand, within emrYat 3992.549 kb on + strand, within emrYat 3992.650 kb on + strand, within emrYat 3992.727 kb on + strand, within emrYat 3992.901 kb on + strandat 3992.916 kb on - strandat 3992.917 kb on + strandat 3992.939 kb on + strandat 3992.939 kb on + strandat 3992.939 kb on + strandat 3993.004 kb on - strandat 3993.006 kb on + strandat 3993.025 kb on - strandat 3993.025 kb on - strandat 3993.089 kb on - strandat 3993.089 kb on - strandat 3993.126 kb on + strandat 3993.131 kb on + strandat 3993.131 kb on + strandat 3993.131 kb on + strandat 3993.132 kb on - strandat 3993.194 kb on - strand, within dsdAat 3993.228 kb on + strand, within dsdAat 3993.229 kb on - strand, within dsdAat 3993.229 kb on - strand, within dsdAat 3993.253 kb on - strand, within dsdAat 3993.253 kb on - strand, within dsdAat 3993.332 kb on - strand, within dsdAat 3993.332 kb on - strand, within dsdAat 3993.374 kb on + strand, within dsdAat 3993.375 kb on - strand, within dsdAat 3993.400 kb on + strand, within dsdAat 3993.400 kb on + strand, within dsdAat 3993.400 kb on + strand, within dsdAat 3993.400 kb on + strand, within dsdAat 3993.401 kb on - strand, within dsdAat 3993.461 kb on - strand, within dsdAat 3993.461 kb on - strand, within dsdAat 3993.461 kb on - strand, within dsdAat 3993.700 kb on - strand, within dsdAat 3993.766 kb on + strand, within dsdAat 3993.766 kb on + strand, within dsdAat 3993.766 kb on + strand, within dsdAat 3993.767 kb on - strand, within dsdAat 3993.767 kb on - strand, within dsdAat 3993.767 kb on - strand, within dsdAat 3993.782 kb on - strand, within dsdAat 3993.803 kb on - strand, within dsdAat 3993.852 kb on + strand, within dsdAat 3993.871 kb on + strand, within dsdA

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,990,547 + emrK NIAGMN_20215 0.30 +1.1
3,990,602 - emrK NIAGMN_20215 0.35 -1.6
3,990,677 + emrK NIAGMN_20215 0.41 +0.8
3,990,828 + emrK NIAGMN_20215 0.54 -2.4
3,990,828 + emrK NIAGMN_20215 0.54 +1.2
3,990,847 + emrK NIAGMN_20215 0.56 -2.4
3,990,848 - emrK NIAGMN_20215 0.56 +0.9
3,990,971 + emrK NIAGMN_20215 0.67 +0.3
3,990,971 + emrK NIAGMN_20215 0.67 +0.9
3,990,996 + emrK NIAGMN_20215 0.69 +3.0
3,991,079 + emrK NIAGMN_20215 0.76 -1.8
3,991,207 - emrK NIAGMN_20215 0.87 +0.6
3,992,189 + emrY NIAGMN_20220 0.54 +0.0
3,992,251 + emrY NIAGMN_20220 0.58 +0.4
3,992,273 + emrY NIAGMN_20220 0.59 -1.6
3,992,416 - emrY NIAGMN_20220 0.69 -0.7
3,992,435 + emrY NIAGMN_20220 0.70 +1.4
3,992,549 + emrY NIAGMN_20220 0.77 +1.0
3,992,650 + emrY NIAGMN_20220 0.84 -2.5
3,992,727 + emrY NIAGMN_20220 0.89 +0.0
3,992,901 + -1.8
3,992,916 - -2.3
3,992,917 + +1.2
3,992,939 + -0.0
3,992,939 + +1.0
3,992,939 + +0.6
3,993,004 - -1.5
3,993,006 + -0.3
3,993,025 - +2.1
3,993,025 - -1.7
3,993,089 - +0.9
3,993,089 - +1.2
3,993,126 + -2.7
3,993,131 + -1.1
3,993,131 + -1.6
3,993,131 + +0.7
3,993,132 - -0.6
3,993,194 - dsdA NIAGMN_20225 0.14 -0.6
3,993,228 + dsdA NIAGMN_20225 0.17 -1.8
3,993,229 - dsdA NIAGMN_20225 0.17 -1.5
3,993,229 - dsdA NIAGMN_20225 0.17 +0.6
3,993,253 - dsdA NIAGMN_20225 0.19 +0.7
3,993,253 - dsdA NIAGMN_20225 0.19 +1.1
3,993,332 - dsdA NIAGMN_20225 0.25 -3.2
3,993,332 - dsdA NIAGMN_20225 0.25 +0.7
3,993,374 + dsdA NIAGMN_20225 0.28 -0.6
3,993,375 - dsdA NIAGMN_20225 0.28 -1.1
3,993,400 + dsdA NIAGMN_20225 0.30 -0.7
3,993,400 + dsdA NIAGMN_20225 0.30 -1.9
3,993,400 + dsdA NIAGMN_20225 0.30 +1.4
3,993,400 + dsdA NIAGMN_20225 0.30 -0.6
3,993,401 - dsdA NIAGMN_20225 0.30 +0.8
3,993,461 - dsdA NIAGMN_20225 0.34 -0.3
3,993,461 - dsdA NIAGMN_20225 0.34 -2.1
3,993,461 - dsdA NIAGMN_20225 0.34 -1.4
3,993,700 - dsdA NIAGMN_20225 0.52 -1.3
3,993,766 + dsdA NIAGMN_20225 0.57 -2.0
3,993,766 + dsdA NIAGMN_20225 0.57 +0.6
3,993,766 + dsdA NIAGMN_20225 0.57 +0.4
3,993,767 - dsdA NIAGMN_20225 0.57 -2.8
3,993,767 - dsdA NIAGMN_20225 0.57 -2.4
3,993,767 - dsdA NIAGMN_20225 0.57 +1.6
3,993,782 - dsdA NIAGMN_20225 0.58 -0.3
3,993,803 - dsdA NIAGMN_20225 0.60 -1.0
3,993,852 + dsdA NIAGMN_20225 0.64 -0.1
3,993,871 + dsdA NIAGMN_20225 0.65 -1.4

Or see this region's nucleotide sequence