Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutR and hemF are separated by 5 nucleotides hemF and amiA are separated by 3 nucleotides amiA and ypeA are separated by 213 nucleotides ypeA and yfeZ overlap by 14 nucleotides
NIAGMN_19860: eutR - HTH-type transcriptional regulator EutR, at 3,923,386 to 3,924,438
eutR
NIAGMN_19865: hemF - oxygen-dependent coproporphyrinogen oxidase, at 3,924,444 to 3,925,343
hemF
NIAGMN_19870: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 3,925,347 to 3,926,216
amiA
NIAGMN_19875: ypeA - GNAT family acetyltransferase, at 3,926,430 to 3,926,855
ypeA
NIAGMN_19880: yfeZ - Inner membrane protein YfeZ, at 3,926,842 to 3,927,291
yfeZ
Position (kb)
3925
3926
3927 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3924.373 kb on + strand at 3924.446 kb on - strand at 3924.464 kb on + strand at 3924.464 kb on + strand at 3924.465 kb on - strand at 3924.467 kb on + strand at 3924.517 kb on + strand at 3924.518 kb on - strand at 3924.626 kb on - strand, within hemF at 3924.640 kb on + strand, within hemF at 3924.684 kb on - strand, within hemF at 3924.684 kb on - strand, within hemF at 3924.706 kb on + strand, within hemF at 3924.706 kb on + strand, within hemF at 3924.707 kb on - strand, within hemF at 3924.707 kb on - strand, within hemF at 3924.707 kb on - strand, within hemF at 3924.718 kb on + strand, within hemF at 3924.740 kb on + strand, within hemF at 3924.741 kb on - strand, within hemF at 3924.741 kb on - strand, within hemF at 3924.774 kb on + strand, within hemF at 3924.774 kb on + strand, within hemF at 3924.775 kb on - strand, within hemF at 3924.775 kb on - strand, within hemF at 3924.830 kb on - strand, within hemF at 3924.830 kb on - strand, within hemF at 3924.836 kb on - strand, within hemF at 3924.875 kb on + strand, within hemF at 3924.876 kb on - strand, within hemF at 3924.940 kb on + strand, within hemF at 3924.941 kb on - strand, within hemF at 3925.042 kb on + strand, within hemF at 3925.043 kb on - strand, within hemF at 3925.043 kb on - strand, within hemF at 3925.043 kb on - strand, within hemF at 3925.063 kb on - strand, within hemF at 3925.208 kb on + strand, within hemF at 3925.209 kb on - strand, within hemF at 3925.241 kb on + strand, within hemF at 3925.241 kb on + strand, within hemF at 3925.242 kb on - strand, within hemF at 3925.242 kb on - strand, within hemF at 3925.242 kb on - strand, within hemF at 3925.343 kb on + strand at 3925.393 kb on + strand at 3925.393 kb on + strand at 3925.568 kb on + strand, within amiA at 3925.568 kb on + strand, within amiA at 3925.596 kb on - strand, within amiA at 3925.628 kb on + strand, within amiA at 3925.665 kb on + strand, within amiA at 3925.666 kb on - strand, within amiA at 3925.732 kb on + strand, within amiA at 3925.733 kb on - strand, within amiA at 3925.749 kb on - strand, within amiA at 3925.829 kb on + strand, within amiA at 3925.850 kb on + strand, within amiA at 3925.906 kb on - strand, within amiA at 3925.929 kb on - strand, within amiA at 3926.197 kb on - strand at 3926.205 kb on - strand at 3926.252 kb on - strand at 3926.252 kb on - strand at 3926.301 kb on + strand at 3926.365 kb on + strand at 3926.366 kb on - strand at 3926.426 kb on + strand at 3926.426 kb on + strand at 3926.537 kb on + strand, within ypeA at 3926.537 kb on + strand, within ypeA at 3926.781 kb on + strand, within ypeA at 3926.781 kb on + strand, within ypeA at 3926.850 kb on + strand at 3926.873 kb on - strand at 3926.921 kb on + strand, within yfeZ at 3926.982 kb on + strand, within yfeZ at 3927.115 kb on + strand, within yfeZ at 3927.146 kb on + strand, within yfeZ at 3927.146 kb on + strand, within yfeZ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,924,373 + +1.1 3,924,446 - +0.6 3,924,464 + +0.1 3,924,464 + -1.2 3,924,465 - +1.6 3,924,467 + +0.6 3,924,517 + +0.7 3,924,518 - -3.0 3,924,626 - hemF NIAGMN_19865 0.20 -0.5 3,924,640 + hemF NIAGMN_19865 0.22 -1.4 3,924,684 - hemF NIAGMN_19865 0.27 -2.6 3,924,684 - hemF NIAGMN_19865 0.27 +1.5 3,924,706 + hemF NIAGMN_19865 0.29 +0.4 3,924,706 + hemF NIAGMN_19865 0.29 +1.0 3,924,707 - hemF NIAGMN_19865 0.29 -0.3 3,924,707 - hemF NIAGMN_19865 0.29 +0.4 3,924,707 - hemF NIAGMN_19865 0.29 +0.7 3,924,718 + hemF NIAGMN_19865 0.30 +0.6 3,924,740 + hemF NIAGMN_19865 0.33 +0.7 3,924,741 - hemF NIAGMN_19865 0.33 +1.2 3,924,741 - hemF NIAGMN_19865 0.33 +1.1 3,924,774 + hemF NIAGMN_19865 0.37 -2.2 3,924,774 + hemF NIAGMN_19865 0.37 -0.8 3,924,775 - hemF NIAGMN_19865 0.37 +0.3 3,924,775 - hemF NIAGMN_19865 0.37 +0.7 3,924,830 - hemF NIAGMN_19865 0.43 -1.2 3,924,830 - hemF NIAGMN_19865 0.43 -0.4 3,924,836 - hemF NIAGMN_19865 0.44 +0.4 3,924,875 + hemF NIAGMN_19865 0.48 -3.2 3,924,876 - hemF NIAGMN_19865 0.48 -0.0 3,924,940 + hemF NIAGMN_19865 0.55 -0.7 3,924,941 - hemF NIAGMN_19865 0.55 +2.3 3,925,042 + hemF NIAGMN_19865 0.66 -1.8 3,925,043 - hemF NIAGMN_19865 0.67 +0.1 3,925,043 - hemF NIAGMN_19865 0.67 -0.1 3,925,043 - hemF NIAGMN_19865 0.67 +0.5 3,925,063 - hemF NIAGMN_19865 0.69 -1.0 3,925,208 + hemF NIAGMN_19865 0.85 +2.1 3,925,209 - hemF NIAGMN_19865 0.85 +0.0 3,925,241 + hemF NIAGMN_19865 0.89 -0.6 3,925,241 + hemF NIAGMN_19865 0.89 +0.0 3,925,242 - hemF NIAGMN_19865 0.89 +0.0 3,925,242 - hemF NIAGMN_19865 0.89 +1.2 3,925,242 - hemF NIAGMN_19865 0.89 +1.5 3,925,343 + -0.4 3,925,393 + -0.8 3,925,393 + -0.7 3,925,568 + amiA NIAGMN_19870 0.25 +0.4 3,925,568 + amiA NIAGMN_19870 0.25 +0.8 3,925,596 - amiA NIAGMN_19870 0.29 -1.0 3,925,628 + amiA NIAGMN_19870 0.32 +1.5 3,925,665 + amiA NIAGMN_19870 0.37 -2.1 3,925,666 - amiA NIAGMN_19870 0.37 +2.5 3,925,732 + amiA NIAGMN_19870 0.44 +0.6 3,925,733 - amiA NIAGMN_19870 0.44 +0.3 3,925,749 - amiA NIAGMN_19870 0.46 +0.9 3,925,829 + amiA NIAGMN_19870 0.55 +0.2 3,925,850 + amiA NIAGMN_19870 0.58 +1.9 3,925,906 - amiA NIAGMN_19870 0.64 +0.7 3,925,929 - amiA NIAGMN_19870 0.67 +2.4 3,926,197 - +0.7 3,926,205 - +1.0 3,926,252 - -0.2 3,926,252 - +0.7 3,926,301 + -2.0 3,926,365 + +1.3 3,926,366 - -0.0 3,926,426 + +0.8 3,926,426 + -0.1 3,926,537 + ypeA NIAGMN_19875 0.25 -0.1 3,926,537 + ypeA NIAGMN_19875 0.25 +1.9 3,926,781 + ypeA NIAGMN_19875 0.82 -1.1 3,926,781 + ypeA NIAGMN_19875 0.82 -2.6 3,926,850 + -2.6 3,926,873 - -1.4 3,926,921 + yfeZ NIAGMN_19880 0.18 +1.4 3,926,982 + yfeZ NIAGMN_19880 0.31 +0.0 3,927,115 + yfeZ NIAGMN_19880 0.61 +0.2 3,927,146 + yfeZ NIAGMN_19880 0.68 -1.4 3,927,146 + yfeZ NIAGMN_19880 0.68 +0.8
Or see this region's nucleotide sequence