Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt srlD and srlB are separated by 3 nucleotides srlB and NIAGMN_18450 are separated by 10 nucleotides NIAGMN_18450 and NIAGMN_18455 are separated by 104 nucleotides NIAGMN_18455 and NIAGMN_18460 are separated by 163 nucleotides
NIAGMN_18440: srlD - sorbitol-6-phosphate dehydrogenase, at 3,649,230 to 3,650,009
srlD
NIAGMN_18445: srlB - PTS glucitol/sorbitol transporter subunit IIA, at 3,650,013 to 3,650,384
srlB
NIAGMN_18450: NIAGMN_18450 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components frag, at 3,650,395 to 3,651,045
_18450
NIAGMN_18455: NIAGMN_18455 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components frag, at 3,651,150 to 3,651,356
_18455
NIAGMN_18460: NIAGMN_18460 - Glucitol/sorbitol permease IIC component, at 3,651,520 to 3,651,915
_18460
Position (kb)
3650
3651
3652 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3649.412 kb on - strand, within srlD at 3649.479 kb on - strand, within srlD at 3649.548 kb on - strand, within srlD at 3649.664 kb on - strand, within srlD at 3649.750 kb on + strand, within srlD at 3649.751 kb on - strand, within srlD at 3649.751 kb on - strand, within srlD at 3649.759 kb on - strand, within srlD at 3649.786 kb on - strand, within srlD at 3649.843 kb on + strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.968 kb on + strand at 3650.211 kb on - strand, within srlB at 3650.211 kb on - strand, within srlB at 3650.270 kb on - strand, within srlB at 3650.376 kb on - strand at 3650.376 kb on - strand at 3650.397 kb on - strand at 3650.406 kb on + strand at 3650.435 kb on - strand at 3650.599 kb on - strand, within NIAGMN_18450 at 3650.599 kb on - strand, within NIAGMN_18450 at 3650.629 kb on + strand, within NIAGMN_18450 at 3650.815 kb on - strand, within NIAGMN_18450 at 3650.834 kb on - strand, within NIAGMN_18450 at 3650.837 kb on - strand, within NIAGMN_18450 at 3650.837 kb on - strand, within NIAGMN_18450 at 3650.954 kb on + strand, within NIAGMN_18450 at 3651.032 kb on + strand at 3651.032 kb on + strand at 3651.033 kb on - strand at 3651.033 kb on - strand at 3651.033 kb on - strand at 3651.033 kb on - strand at 3651.033 kb on - strand at 3651.033 kb on - strand at 3651.036 kb on + strand at 3651.037 kb on - strand at 3651.037 kb on - strand at 3651.051 kb on - strand at 3651.051 kb on - strand at 3651.051 kb on - strand at 3651.088 kb on - strand at 3651.088 kb on - strand at 3651.090 kb on + strand at 3651.131 kb on - strand at 3651.193 kb on - strand, within NIAGMN_18455 at 3651.204 kb on + strand, within NIAGMN_18455 at 3651.270 kb on - strand, within NIAGMN_18455 at 3651.271 kb on + strand, within NIAGMN_18455 at 3651.272 kb on - strand, within NIAGMN_18455 at 3651.272 kb on - strand, within NIAGMN_18455 at 3651.272 kb on - strand, within NIAGMN_18455 at 3651.272 kb on - strand, within NIAGMN_18455 at 3651.347 kb on + strand at 3651.480 kb on + strand at 3651.600 kb on + strand, within NIAGMN_18460 at 3651.600 kb on + strand, within NIAGMN_18460 at 3651.600 kb on + strand, within NIAGMN_18460 at 3651.601 kb on - strand, within NIAGMN_18460 at 3651.601 kb on - strand, within NIAGMN_18460 at 3651.601 kb on - strand, within NIAGMN_18460 at 3651.601 kb on - strand, within NIAGMN_18460 at 3651.602 kb on + strand, within NIAGMN_18460 at 3651.602 kb on + strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.603 kb on - strand, within NIAGMN_18460 at 3651.620 kb on + strand, within NIAGMN_18460 at 3651.621 kb on - strand, within NIAGMN_18460 at 3651.632 kb on + strand, within NIAGMN_18460 at 3651.632 kb on + strand, within NIAGMN_18460 at 3651.633 kb on - strand, within NIAGMN_18460 at 3651.662 kb on - strand, within NIAGMN_18460 at 3651.662 kb on - strand, within NIAGMN_18460 at 3651.704 kb on - strand, within NIAGMN_18460 at 3651.709 kb on + strand, within NIAGMN_18460 at 3651.710 kb on - strand, within NIAGMN_18460 at 3651.710 kb on - strand, within NIAGMN_18460 at 3651.759 kb on + strand, within NIAGMN_18460 at 3651.759 kb on + strand, within NIAGMN_18460 at 3651.900 kb on + strand at 3651.910 kb on + strand at 3651.910 kb on + strand at 3651.910 kb on + strand at 3651.911 kb on - strand at 3651.911 kb on - strand at 3651.911 kb on - strand at 3652.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,649,412 - srlD NIAGMN_18440 0.23 +0.0 3,649,479 - srlD NIAGMN_18440 0.32 +0.5 3,649,548 - srlD NIAGMN_18440 0.41 +2.1 3,649,664 - srlD NIAGMN_18440 0.56 -0.8 3,649,750 + srlD NIAGMN_18440 0.67 +0.8 3,649,751 - srlD NIAGMN_18440 0.67 -1.9 3,649,751 - srlD NIAGMN_18440 0.67 +1.1 3,649,759 - srlD NIAGMN_18440 0.68 +0.8 3,649,786 - srlD NIAGMN_18440 0.71 +0.2 3,649,843 + srlD NIAGMN_18440 0.79 -1.3 3,649,844 - srlD NIAGMN_18440 0.79 +1.7 3,649,844 - srlD NIAGMN_18440 0.79 -1.6 3,649,844 - srlD NIAGMN_18440 0.79 -0.2 3,649,844 - srlD NIAGMN_18440 0.79 +1.0 3,649,968 + -1.4 3,650,211 - srlB NIAGMN_18445 0.53 -1.6 3,650,211 - srlB NIAGMN_18445 0.53 -0.1 3,650,270 - srlB NIAGMN_18445 0.69 -0.4 3,650,376 - +0.1 3,650,376 - -3.1 3,650,397 - +0.1 3,650,406 + +0.5 3,650,435 - +0.3 3,650,599 - NIAGMN_18450 0.31 +1.5 3,650,599 - NIAGMN_18450 0.31 +0.2 3,650,629 + NIAGMN_18450 0.36 -1.4 3,650,815 - NIAGMN_18450 0.65 -1.1 3,650,834 - NIAGMN_18450 0.67 +1.0 3,650,837 - NIAGMN_18450 0.68 -0.7 3,650,837 - NIAGMN_18450 0.68 -3.8 3,650,954 + NIAGMN_18450 0.86 +1.0 3,651,032 + +0.2 3,651,032 + -2.3 3,651,033 - -0.5 3,651,033 - +2.3 3,651,033 - -0.9 3,651,033 - -3.6 3,651,033 - -0.6 3,651,033 - -1.1 3,651,036 + +1.6 3,651,037 - +1.5 3,651,037 - +1.6 3,651,051 - +0.9 3,651,051 - -0.1 3,651,051 - +0.1 3,651,088 - -1.1 3,651,088 - +0.2 3,651,090 + -0.8 3,651,131 - +0.7 3,651,193 - NIAGMN_18455 0.21 +1.1 3,651,204 + NIAGMN_18455 0.26 -4.2 3,651,270 - NIAGMN_18455 0.58 +0.5 3,651,271 + NIAGMN_18455 0.58 +1.2 3,651,272 - NIAGMN_18455 0.59 -0.8 3,651,272 - NIAGMN_18455 0.59 +1.3 3,651,272 - NIAGMN_18455 0.59 -0.8 3,651,272 - NIAGMN_18455 0.59 -0.3 3,651,347 + -0.6 3,651,480 + +2.2 3,651,600 + NIAGMN_18460 0.20 +0.6 3,651,600 + NIAGMN_18460 0.20 +0.8 3,651,600 + NIAGMN_18460 0.20 -1.1 3,651,601 - NIAGMN_18460 0.20 +2.6 3,651,601 - NIAGMN_18460 0.20 +1.1 3,651,601 - NIAGMN_18460 0.20 +2.5 3,651,601 - NIAGMN_18460 0.20 +0.2 3,651,602 + NIAGMN_18460 0.21 +1.2 3,651,602 + NIAGMN_18460 0.21 +1.0 3,651,603 - NIAGMN_18460 0.21 +0.9 3,651,603 - NIAGMN_18460 0.21 +0.8 3,651,603 - NIAGMN_18460 0.21 -2.0 3,651,603 - NIAGMN_18460 0.21 -3.6 3,651,603 - NIAGMN_18460 0.21 +1.8 3,651,603 - NIAGMN_18460 0.21 +0.8 3,651,603 - NIAGMN_18460 0.21 +0.2 3,651,620 + NIAGMN_18460 0.25 -0.2 3,651,621 - NIAGMN_18460 0.26 -0.1 3,651,632 + NIAGMN_18460 0.28 -2.1 3,651,632 + NIAGMN_18460 0.28 -2.8 3,651,633 - NIAGMN_18460 0.29 -2.5 3,651,662 - NIAGMN_18460 0.36 -1.2 3,651,662 - NIAGMN_18460 0.36 +2.1 3,651,704 - NIAGMN_18460 0.46 +0.0 3,651,709 + NIAGMN_18460 0.48 -2.0 3,651,710 - NIAGMN_18460 0.48 +0.0 3,651,710 - NIAGMN_18460 0.48 +0.1 3,651,759 + NIAGMN_18460 0.60 -1.0 3,651,759 + NIAGMN_18460 0.60 +0.1 3,651,900 + +0.9 3,651,910 + -1.7 3,651,910 + -0.2 3,651,910 + -1.4 3,651,911 - +0.3 3,651,911 - +0.3 3,651,911 - +1.2 3,652,042 - +0.8
Or see this region's nucleotide sequence