Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysH and hokG are separated by 263 nucleotides hokG and cas3 are separated by 191 nucleotides
NIAGMN_18150: cysH - phosphoadenosine phosphosulfate reductase, at 3,593,237 to 3,593,971
cysH
NIAGMN_18160: hokG - Putative protein HokG, at 3,594,235 to 3,594,387
hokG
NIAGMN_18165: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,594,579 to 3,597,278
cas3
Position (kb)
3594
3595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3593.236 kb on + strand at 3593.237 kb on - strand at 3593.237 kb on - strand at 3593.237 kb on - strand at 3593.237 kb on - strand at 3593.237 kb on - strand at 3593.237 kb on - strand at 3593.281 kb on - strand at 3593.379 kb on - strand, within cysH at 3593.379 kb on - strand, within cysH at 3593.379 kb on - strand, within cysH at 3593.382 kb on + strand, within cysH at 3593.383 kb on - strand, within cysH at 3593.477 kb on + strand, within cysH at 3593.496 kb on - strand, within cysH at 3593.504 kb on - strand, within cysH at 3593.546 kb on + strand, within cysH at 3593.627 kb on + strand, within cysH at 3593.677 kb on + strand, within cysH at 3593.768 kb on - strand, within cysH at 3593.805 kb on + strand, within cysH at 3593.807 kb on + strand, within cysH at 3593.807 kb on + strand, within cysH at 3593.813 kb on + strand, within cysH at 3593.861 kb on + strand, within cysH at 3593.973 kb on + strand at 3593.988 kb on + strand at 3594.074 kb on - strand at 3594.082 kb on + strand at 3594.082 kb on + strand at 3594.082 kb on + strand at 3594.083 kb on - strand at 3594.165 kb on + strand at 3594.166 kb on - strand at 3594.193 kb on - strand at 3594.193 kb on - strand at 3594.208 kb on - strand at 3594.239 kb on + strand at 3594.240 kb on - strand at 3594.248 kb on - strand at 3594.266 kb on + strand, within hokG at 3594.266 kb on + strand, within hokG at 3594.266 kb on + strand, within hokG at 3594.266 kb on + strand, within hokG at 3594.266 kb on + strand, within hokG at 3594.267 kb on - strand, within hokG at 3594.269 kb on + strand, within hokG at 3594.269 kb on + strand, within hokG at 3594.269 kb on + strand, within hokG at 3594.270 kb on - strand, within hokG at 3594.279 kb on + strand, within hokG at 3594.279 kb on + strand, within hokG at 3594.279 kb on + strand, within hokG at 3594.279 kb on + strand, within hokG at 3594.280 kb on - strand, within hokG at 3594.280 kb on - strand, within hokG at 3594.317 kb on + strand, within hokG at 3594.317 kb on + strand, within hokG at 3594.317 kb on + strand, within hokG at 3594.318 kb on - strand, within hokG at 3594.318 kb on - strand, within hokG at 3594.346 kb on - strand, within hokG at 3594.385 kb on + strand at 3594.385 kb on + strand at 3594.385 kb on + strand at 3594.385 kb on + strand at 3594.475 kb on + strand at 3594.478 kb on - strand at 3594.497 kb on - strand at 3594.556 kb on - strand at 3594.584 kb on + strand at 3594.730 kb on + strand at 3595.033 kb on + strand, within cas3 at 3595.033 kb on + strand, within cas3 at 3595.235 kb on + strand, within cas3 at 3595.236 kb on - strand, within cas3 at 3595.316 kb on + strand, within cas3 at 3595.317 kb on - strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,593,236 + +0.7 3,593,237 - -0.4 3,593,237 - +0.3 3,593,237 - -1.9 3,593,237 - +0.3 3,593,237 - -0.2 3,593,237 - -0.6 3,593,281 - -0.1 3,593,379 - cysH NIAGMN_18150 0.19 +2.0 3,593,379 - cysH NIAGMN_18150 0.19 -1.3 3,593,379 - cysH NIAGMN_18150 0.19 +0.5 3,593,382 + cysH NIAGMN_18150 0.20 -0.4 3,593,383 - cysH NIAGMN_18150 0.20 -2.9 3,593,477 + cysH NIAGMN_18150 0.33 +0.3 3,593,496 - cysH NIAGMN_18150 0.35 -2.1 3,593,504 - cysH NIAGMN_18150 0.36 -0.6 3,593,546 + cysH NIAGMN_18150 0.42 +0.6 3,593,627 + cysH NIAGMN_18150 0.53 -0.7 3,593,677 + cysH NIAGMN_18150 0.60 +1.2 3,593,768 - cysH NIAGMN_18150 0.72 +0.7 3,593,805 + cysH NIAGMN_18150 0.77 +0.5 3,593,807 + cysH NIAGMN_18150 0.78 +0.2 3,593,807 + cysH NIAGMN_18150 0.78 -0.1 3,593,813 + cysH NIAGMN_18150 0.78 +0.4 3,593,861 + cysH NIAGMN_18150 0.85 +1.0 3,593,973 + -0.2 3,593,988 + +1.2 3,594,074 - +0.9 3,594,082 + -2.0 3,594,082 + +0.3 3,594,082 + -0.5 3,594,083 - +1.2 3,594,165 + -1.4 3,594,166 - -0.4 3,594,193 - +0.7 3,594,193 - +0.8 3,594,208 - +0.4 3,594,239 + +1.0 3,594,240 - +0.6 3,594,248 - -1.2 3,594,266 + hokG NIAGMN_18160 0.20 -0.7 3,594,266 + hokG NIAGMN_18160 0.20 +0.7 3,594,266 + hokG NIAGMN_18160 0.20 +0.6 3,594,266 + hokG NIAGMN_18160 0.20 +0.4 3,594,266 + hokG NIAGMN_18160 0.20 +1.1 3,594,267 - hokG NIAGMN_18160 0.21 +0.9 3,594,269 + hokG NIAGMN_18160 0.22 +1.1 3,594,269 + hokG NIAGMN_18160 0.22 +0.8 3,594,269 + hokG NIAGMN_18160 0.22 +0.3 3,594,270 - hokG NIAGMN_18160 0.23 +0.4 3,594,279 + hokG NIAGMN_18160 0.29 +1.2 3,594,279 + hokG NIAGMN_18160 0.29 -1.3 3,594,279 + hokG NIAGMN_18160 0.29 -0.8 3,594,279 + hokG NIAGMN_18160 0.29 -0.6 3,594,280 - hokG NIAGMN_18160 0.29 +0.4 3,594,280 - hokG NIAGMN_18160 0.29 -0.1 3,594,317 + hokG NIAGMN_18160 0.54 +0.4 3,594,317 + hokG NIAGMN_18160 0.54 -1.4 3,594,317 + hokG NIAGMN_18160 0.54 +0.8 3,594,318 - hokG NIAGMN_18160 0.54 +1.5 3,594,318 - hokG NIAGMN_18160 0.54 +1.7 3,594,346 - hokG NIAGMN_18160 0.73 -2.0 3,594,385 + +0.5 3,594,385 + +0.4 3,594,385 + -0.7 3,594,385 + -0.6 3,594,475 + +1.0 3,594,478 - +0.5 3,594,497 - +0.5 3,594,556 - +0.3 3,594,584 + +1.6 3,594,730 + +0.9 3,595,033 + cas3 NIAGMN_18165 0.17 +0.8 3,595,033 + cas3 NIAGMN_18165 0.17 -0.3 3,595,235 + cas3 NIAGMN_18165 0.24 +0.4 3,595,236 - cas3 NIAGMN_18165 0.24 +1.9 3,595,316 + cas3 NIAGMN_18165 0.27 +1.0 3,595,317 - cas3 NIAGMN_18165 0.27 -2.1
Or see this region's nucleotide sequence