Strain Fitness in Escherichia coli ECRC102 around NIAGMN_17280

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiH and ubiI are separated by 23 nucleotidesubiI and gcvT are separated by 447 nucleotides NIAGMN_17275: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 3,411,083 to 3,412,261 ubiH NIAGMN_17280: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 3,412,285 to 3,413,487 ubiI NIAGMN_17285: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 3,413,935 to 3,415,029 gcvT Position (kb) 3412 3413 3414Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3412.203 kb on + strandat 3412.203 kb on + strandat 3412.204 kb on - strandat 3412.270 kb on - strandat 3412.270 kb on - strandat 3412.273 kb on - strandat 3412.279 kb on + strandat 3412.279 kb on + strandat 3412.301 kb on + strandat 3412.301 kb on + strandat 3412.662 kb on + strand, within ubiIat 3412.743 kb on + strand, within ubiIat 3412.749 kb on - strand, within ubiIat 3412.859 kb on + strand, within ubiIat 3413.202 kb on + strand, within ubiIat 3413.428 kb on - strandat 3413.507 kb on + strandat 3413.508 kb on - strandat 3413.515 kb on - strandat 3413.764 kb on - strandat 3413.768 kb on + strandat 3413.885 kb on + strandat 3413.886 kb on - strandat 3413.956 kb on + strandat 3414.053 kb on + strand, within gcvTat 3414.054 kb on - strand, within gcvTat 3414.054 kb on - strand, within gcvTat 3414.058 kb on - strand, within gcvTat 3414.087 kb on + strand, within gcvTat 3414.088 kb on - strand, within gcvTat 3414.088 kb on - strand, within gcvTat 3414.088 kb on - strand, within gcvTat 3414.088 kb on - strand, within gcvTat 3414.137 kb on - strand, within gcvTat 3414.222 kb on - strand, within gcvTat 3414.222 kb on - strand, within gcvTat 3414.241 kb on + strand, within gcvTat 3414.241 kb on + strand, within gcvTat 3414.242 kb on - strand, within gcvTat 3414.374 kb on + strand, within gcvTat 3414.414 kb on + strand, within gcvT

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,412,203 + -1.7
3,412,203 + -0.7
3,412,204 - +0.6
3,412,270 - +1.2
3,412,270 - -0.5
3,412,273 - -0.9
3,412,279 + +1.7
3,412,279 + -1.2
3,412,301 + +0.4
3,412,301 + -1.4
3,412,662 + ubiI NIAGMN_17280 0.31 -0.2
3,412,743 + ubiI NIAGMN_17280 0.38 +0.1
3,412,749 - ubiI NIAGMN_17280 0.39 -0.9
3,412,859 + ubiI NIAGMN_17280 0.48 -0.2
3,413,202 + ubiI NIAGMN_17280 0.76 -0.4
3,413,428 - -2.3
3,413,507 + -0.2
3,413,508 - -0.7
3,413,515 - +0.4
3,413,764 - -2.2
3,413,768 + -3.1
3,413,885 + +1.9
3,413,886 - +3.1
3,413,956 + -0.3
3,414,053 + gcvT NIAGMN_17285 0.11 -1.8
3,414,054 - gcvT NIAGMN_17285 0.11 -0.5
3,414,054 - gcvT NIAGMN_17285 0.11 -2.0
3,414,058 - gcvT NIAGMN_17285 0.11 +0.6
3,414,087 + gcvT NIAGMN_17285 0.14 +0.8
3,414,088 - gcvT NIAGMN_17285 0.14 -1.2
3,414,088 - gcvT NIAGMN_17285 0.14 -0.7
3,414,088 - gcvT NIAGMN_17285 0.14 +2.3
3,414,088 - gcvT NIAGMN_17285 0.14 +1.0
3,414,137 - gcvT NIAGMN_17285 0.18 -3.3
3,414,222 - gcvT NIAGMN_17285 0.26 -1.8
3,414,222 - gcvT NIAGMN_17285 0.26 -3.3
3,414,241 + gcvT NIAGMN_17285 0.28 -1.8
3,414,241 + gcvT NIAGMN_17285 0.28 -3.4
3,414,242 - gcvT NIAGMN_17285 0.28 -0.6
3,414,374 + gcvT NIAGMN_17285 0.40 +0.2
3,414,414 + gcvT NIAGMN_17285 0.44 +0.5

Or see this region's nucleotide sequence